ERLEC1 (NM_015701) Human Tagged ORF Clone

CAT#: RC201486

ERLEC1 (Myc-DDK-tagged)-Human endoplasmic reticulum lectin 1 (ERLEC1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414394) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201486 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ERLEC1 protein (Cat# TP301486). The protein was produced from HEK293T cells transfected with ERLEC1 cDNA clone (Cat# RC201486) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ERLEC1
• Synonyms: C2orf30; CIM; CL24936; CL25084; HEL117; XTP3-B; XTP3TPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201486 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAAGGAGGCGGCGGCGTACGGAGTCTGGTCCCGGGCGGGCCGGTGTTACTGGTCCTCTGCGGCC
  TCCTGGAGGCGTCCGGCGGCGGCCGAGCCCTTCCTCAACTCAGCGATGACATCCCTTTCCGAGTCAACTG
  GCCCGGCACCGAGTTCTCTCTGCCCACAACTGGAGTTTTATATAAAGAAGATAATTATGTCATCATGACA
  ACTGCACATAAAGAAAAATATAAATGCATACTTCCCCTTGTGACAAGTGGGGATGAGGAAGAAGAAAAGG
  ATTATAAAGGCCCTAATCCAAGAGAGCTTTTGGAGCCACTATTTAAACAAAGCAGTTGTTCCTACAGAAT
  TGAGTCTTATTGGACTTACGAAGTATGTCATGGAAAACACATTCGGCAGTACCATGAAGAGAAAGAAACT
  GGTCAGAAAATAAATATTCACGAGTACTACCTTGGGAATATGTTGGCCAAGAACCTTCTATTTGAAAAAG
  AACGAGAAGCAGAAGAAAAGGAAAAATCAAATGAGATTCCCACTAAAAATATCGAAGGTCAGATGACACC
  ATACTATCCTGTGGGAATGGGAAATGGTACACCTTGTAGTTTGAAACAGAACCGGCCCAGATCAAGTACT
  GTGATGTACATATGTCATCCTGAATCTAAGCATGAAATTCTTTCAGTAGCTGAAGTTACAACTTGTGAAT
  ATGAAGTTGTCATTTTGACACCACTCTTGTGCAGTCATCCTAAATATAGGTTCAGAGCATCTCCTGTGAA
  TGACATATTTTGTCAATCACTGCCAGGATCTCCATTTAAGCCCCTCACCCTGAGGCAGCTGGAGCAGCAG
  GAAGAAATACTAAGGGTGCCTTTTAGGAGAAATAAAGAGGAAGATTTGCAATCAACTAAAGAAGAGAGAT
  TTCCAGCGATCCACAAGTCGATTGCTATTGGCTCTCAGCCAGTGCTCACTGTTGGGACAACCCACATATC
  CAAATTGACAGATGACCAACTCATAAAAGAGTTTCTTAGTGGTTCTTACTGCTTTCGTGGGGGTGTCGGT
  TGGTGGAAATATGAATTCTGCTATGGCAAACATGTACATCAATACCATGAGGACAAGGATAGTGGGAAAA
  CCTCTGTGGTTGTCGGGACATGGAACCAAGAAGAGCATATTGAATGGGCTAAGAAGAATACTGCTAGAGC
  TTATCATCTTCAAGACGATGGTACCCAGACAGTCAGGATGGTGTCACATTTTTATGGAAATGGAGATATT
  TGTGATATAACTGACAAACCAAGACAGGTGACTGTAAAACTAAAGTGCAAAGAATCAGATTCACCTCATG
  CTGTTACTGTATATATGCTAGAGCCTCACTCCTGTCAATATATTCTTGGGGTTGAATCTCCAGTGATCTG
  TAAAATCTTAGATACAGCAGATGAAAATGGACTTCTTTCTCTCCCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201486 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEGGGGVRSLVPGGPVLLVLCGLLEASGGGRALPQLSDDIPFRVNWPGTEFSLPTTGVLYKEDNYVIMT
  TAHKEKYKCILPLVTSGDEEEEKDYKGPNPRELLEPLFKQSSCSYRIESYWTYEVCHGKHIRQYHEEKET
  GQKINIHEYYLGNMLAKNLLFEKEREAEEKEKSNEIPTKNIEGQMTPYYPVGMGNGTPCSLKQNRPRSST
  VMYICHPESKHEILSVAEVTTCEYEVVILTPLLCSHPKYRFRASPVNDIFCQSLPGSPFKPLTLRQLEQQ
  EEILRVPFRRNKEEDLQSTKEERFPAIHKSIAIGSQPVLTVGTTHISKLTDDQLIKEFLSGSYCFRGGVG
  WWKYEFCYGKHVHQYHEDKDSGKTSVVVGTWNQEEHIEWAKKNTARAYHLQDDGTQTVRMVSHFYGNGDI
  CDITDKPRQVTVKLKCKESDSPHAVTVYMLEPHSCQYILGVESPVICKILDTADENGLLSLPN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015701
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015701.1, NM_015701.2, NM_015701.3, NM_015701.4, NP_056516.2
• RefSeq Size: 2605 bp
• RefSeq ORF: 1452 bp
• Locus ID: 27248
• Protein Families: Secreted Protein
• MW: 54.9 kDa
• Gene Summary: This gene encodes a resident endoplasmic reticulum (ER) protein that functions in N-glycan recognition. This protein is thought to be involved in ER-associated degradation via its interaction with the membrane-associated ubiquitin ligase complex. It also functions as a regulator of multiple cellular stress-response pathways in a manner that promotes metastatic cell survival. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 21. [provided by RefSeq, Aug 2011]