RAD54B (NM_012415) Human Tagged ORF Clone

CAT#: RC201513

RAD54B (Myc-DDK-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RAD54B
• Synonyms: RDH54
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201513 representing NM_012415
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACGATCTGCAGCACCAAGTCAGTTGCAGGGGAATTCCTTCAAAAAACCAAAATTTATACCTCCAG
  GAAGAAGTAATCCAGGTCTGAATGAAGAGATTACAAAACTGAATCCAGATATAAAATTATTTGAGGGTGT
  TGCAATTAATAACACCTTTCTCCCGTCACAAAATGATCTTAGAATATGCAGTTTAAATCTGCCTAGTGAA
  GAAAGTACTAGAGAAATCAATAACAGAGATAATTGCAGTGGAAAATATTGTTTTGAAGCACCTACACTGG
  CAACATTAGATCCACCTCATACAGTTCATTCGGCTCCTAAAGAAGTAGCAGTGTCCAAGGAACAAGAAGA
  GAAATCTGATAGCCTAGTTAAATATTTCAGTGTTGTTTGGTGTAAGCCTTCAAAGAAAAAACATAAAAAG
  TGGGAAGGTGATGCTGTTCTTATTGTAAAAGGAAAGTCATTTATATTAAAGAATTTGGAAGGCAAAGACA
  TTGGAAGAGGCATTGGTTATAAATTCAAAGAGCTTGAAAAGATTGAAGAGGGCCAAACACTGATGATTTG
  TGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGATGACTTCAGCAGTGGCAGGTGTTTTCAGCTT
  GGAGGAGGAAGTACTGCTATCTCGCATTCTTCTCAGGTTGCCAGGAAATGTTTCTCTAACCCTTTCAAAA
  GTGTTTGTAAACCAAGTTCAAAGGAAAATAGACAGAATGATTTCCAAAATTGCAAACCACGCCATGACCC
  ATATACGCCAAATTCCCTCGTTATGCCACGACCAGATAAGAATCACCAGTGGGTATTCAATAAGAACTGT
  TTCCCTCTTGTGGATGTAGTGATTGATCCTTACCTTGTATATCATCTTCGACCACATCAGAAAGAAGGAA
  TCATATTCCTTTATGAATGTGTAATGGGAATGAGAATGAATGGCAGATGTGGAGCTATTCTTGCTGATGA
  AATGGGTTTAGGGAAGACATTGCAATGTATTTCGCTCATCTGGACCCTGCAGTGTCAGGGACCCTATGGA
  GGCAAGCCAGTAATAAAGAAGACACTAATTGTCACACCTGGAAGCTTGGTGAATAATTGGAAGAAAGAAT
  TTCAAAAATGGCTAGGAAGTGAAAGGATCAAGATATTTACTGTTGATCAGGACCACAAAGTTGAAGAATT
  CATCAAGTCTATATTTTATTCTGTTCTTATTATCAGTTATGAAATGTTACTTCGTTCCCTGGATCAAATT
  AAGAATATAAAATTTGATCTTCTAATCTGTGACGAGGGGCATCGTTTGAAGAACAGTGCCATTAAGACAA
  CTACAGCCCTCATTAGCCTTTCTTGTGAGAAAAGAATAATTCTAACTGGTACTCCAATTCAGAATGATCT
  GCAAGAATTTTTTGCATTAATTGATTTTGTAAATCCAGGAATATTAGGCTCTTTGTCATCTTATAGGAAA
  ATATATGAAGAACCCATCATTTTATCGAGAGAACCTTCTGCTTCTGAGGAAGAAAAGGAGTTAGGAGAAA
  GAAGAGCAGCTGAACTTACTTGCCTCACTGGACTCTTTATCCTTAGAAGAACCCAAGAAATTATAAATAA
  ATATCTCCCACCTAAAATAGAGAATGTTGTCTTTTGCCGACCAGGAGCACTACAGATTGAGCTTTATCGA
  AAGCTGTTAAATTCTCAGGTTGTCAGGTTCTGCCTTCAAGGGTTGTTGGAAAATAGTCCCCATCTAATAT
  GTATAGGAGCTCTTAAAAAACTGTGCAATCACCCCTGCCTTTTGTTCAACTCTATAAAGGAAAAGGAATG
  TAGCTCAACTTGTGATAAAAATGAAGAAAAGAGTCTATACAAAGGCTTGCTAAGTGTGTTTCCTGCTGAC
  TACAACCCTCTCCTGTTTACTGAAAAGGAGTCAGGAAAACTACAGGTGTTGTCCAAGCTCTTAGCGGTTA
  TCCACGAACTTCGACCTACTGAAAAGGTGGTGTTGGTATCCAACTATACACAAACCTTGAACATTTTACA
  AGAAGTATGTAAGCGTCATGGATATGCTTATACAAGACTTGATGGACAAACACCAATCTCTCAAAGGCAG
  CAGATTGTTGATGGCTTTAACAGTCAACACTCTTCTTTTTTTATTTTTTTGTTAAGTTCAAAAGCTGGTG
  GTGTAGGACTTAACCTCATTGGAGGATCTCACTTAATTCTCTATGACATTGATTGGAATCCAGCCACTGA
  CATTCAGGCAATGTCTAGAGTATGGAGAGATGGTCAGAAATATCCTGTACATATTTACAGACTCCTAACT
  ACAGGTACAATAGAAGAAAAGATCTATCAAAGGCAGATCAGTAAGCAAGGTCTTTGTGGGGCAGTTGTCG
  ACCTCACCAAGACATCTGAACATATTCAGTTTTCAGTAGAAGAACTTAAAAATTTGTTCACATTACATGA
  AAGTTCAGATTGTGTTACTCATGATCTGCTTGACTGTGAGTGTACAGGAGAAGAAGTTCATACAGGTGAT
  TCGTTGGAAAAATTCATTGTCTCTAGAGATTGTCAGCTTGGTCCACATCACCAGAAATCTAACTCCCTGA
  AACCTCTTTCTATGTCCCAGCTGAAGCAATGGAAACATTTTTCTGGAGATCATTTAAATCTTACAGATCC
  TTTTCTTGAAAGAATAACAGAAAATGTGTCATTCATTTTTCAGAATATAACCACTCAAGCTACTGGCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201513 representing NM_012415
  Red=Cloning site Green=Tags(s)
  
  MRRSAAPSQLQGNSFKKPKFIPPGRSNPGLNEEITKLNPDIKLFEGVAINNTFLPSQNDLRICSLNLPSE
  ESTREINNRDNCSGKYCFEAPTLATLDPPHTVHSAPKEVAVSKEQEEKSDSLVKYFSVVWCKPSKKKHKK
  WEGDAVLIVKGKSFILKNLEGKDIGRGIGYKFKELEKIEEGQTLMICGKEIEVMGVISPDDFSSGRCFQL
  GGGSTAISHSSQVARKCFSNPFKSVCKPSSKENRQNDFQNCKPRHDPYTPNSLVMPRPDKNHQWVFNKNC
  FPLVDVVIDPYLVYHLRPHQKEGIIFLYECVMGMRMNGRCGAILADEMGLGKTLQCISLIWTLQCQGPYG
  GKPVIKKTLIVTPGSLVNNWKKEFQKWLGSERIKIFTVDQDHKVEEFIKSIFYSVLIISYEMLLRSLDQI
  KNIKFDLLICDEGHRLKNSAIKTTTALISLSCEKRIILTGTPIQNDLQEFFALIDFVNPGILGSLSSYRK
  IYEEPIILSREPSASEEEKELGERRAAELTCLTGLFILRRTQEIINKYLPPKIENVVFCRPGALQIELYR
  KLLNSQVVRFCLQGLLENSPHLICIGALKKLCNHPCLLFNSIKEKECSSTCDKNEEKSLYKGLLSVFPAD
  YNPLLFTEKESGKLQVLSKLLAVIHELRPTEKVVLVSNYTQTLNILQEVCKRHGYAYTRLDGQTPISQRQ
  QIVDGFNSQHSSFFIFLLSSKAGGVGLNLIGGSHLILYDIDWNPATDIQAMSRVWRDGQKYPVHIYRLLT
  TGTIEEKIYQRQISKQGLCGAVVDLTKTSEHIQFSVEELKNLFTLHESSDCVTHDLLDCECTGEEVHTGD
  SLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIFQNITTQATGT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012415
• ORF Size: 2730 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012415.1, NM_012415.2, NM_012415.3, NP_036547.1
• RefSeq Size: 3074
• RefSeq ORF: 2733
• Locus ID: 25788
• Domains: SNF2_N, DEAD, helicase_C
• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination
• MW: 103 kDa
• Gene Summary: The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008]