FAM50B (NM_012135) Human Tagged ORF Clone

CAT#: RC201531

FAM50B (Myc-DDK-tagged)-Human family with sequence similarity 50, member B (FAM50B)

Product Images

Western blot validation of overexpression lysate (Cat# LY415961) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201531 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FAM50B protein (Cat# TP301531). The protein was produced from HEK293T cells transfected with FAM50B cDNA clone (Cat# RC201531) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM50B
• Synonyms: D6S2654E; X5L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201531 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGTACAAGGGCACCATGCGCGAGGCAGGCCGTGCCATGCACCTCCTCAAGAAGCGCGAAAGGC
  AGCGGGAGCAGATGGAGGTGCTGAAGCAGCGCATCGCCGAGGAGACCATCCTCAAGTCGCAGGTGGACAA
  GAGGTTCTCGGCGCATTACGACGCCGTGGAGGCCGAGCTGAAGTCCAGCACGGTGGGCCTGGTGACCCTG
  AACGACATGAAGGCCCGGCAGGAGGCCCTGGTCAGGGAGCGCGAGCGGCAGCTGGCCAAGCGCCAGCACC
  TGGAGGAGCAGCGGCTGCAGCAGGAGCGGCAGCGGGAGCAGGAGCAGCGGCGCGAGCGCAAGCGTAAGAT
  CTCCTGCCTGTCCTTTGCACTAGACGACCTCGATGACCAGGCCGACGCGGCCGAGGCCAGGCGCGCCGGA
  AACCTGGGCAAGAACCCCGACGTGGACACCAGCTTCCTGCCAGACCGCGACCGCGAGGAGGAGGAGAACC
  GGCTCCGAGAGGAGCTGCGCCAAGAGTGGGAGGCGCAGCGCGAGAAAGTGAAGGACGAGGAGATGGAGGT
  CACCTTCAGCTACTGGGACGGCTCGGGCCACCGGCGCACGGTGCGGGTGCGCAAGGGCAACACGGTGCAG
  CAGTTCCTGAAGAAGGCGCTGCAGGGGCTGCGCAAGGACTTCCTGGAGCTGCGCTCCGCCGGCGTGGAGC
  AGCTCATGTTCATCAAGGAGGACCTCATCCTGCCGCACTACCACACCTTCTACGACTTCATCATCGCCAG
  GGCGAGGGGCAAGAGCGGGCCGCTCTTCAGCTTCGATGTGCACGATGACGTGCGCCTGCTCAGCGACGCC
  ACCATGGAGAAGGACGAGTCGCACGCGGGCAAGGTGGTGCTGCGCAGCTGGTACGAGAAGAACAAGCACA
  TCTTCCCCGCCAGCCGCTGGGAGGCCTATGACCCCGAGAAGAAGTGGGACAAGTACACCATCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201531 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQYKGTMREAGRAMHLLKKRERQREQMEVLKQRIAEETILKSQVDKRFSAHYDAVEAELKSSTVGLVTL
  NDMKARQEALVRERERQLAKRQHLEEQRLQQERQREQEQRRERKRKISCLSFALDDLDDQADAAEARRAG
  NLGKNPDVDTSFLPDRDREEEENRLREELRQEWEAQREKVKDEEMEVTFSYWDGSGHRRTVRVRKGNTVQ
  QFLKKALQGLRKDFLELRSAGVEQLMFIKEDLILPHYHTFYDFIIARARGKSGPLFSFDVHDDVRLLSDA
  TMEKDESHAGKVVLRSWYEKNKHIFPASRWEAYDPEKKWDKYTIR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012135
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012135.1, NM_012135.2, NP_036267.1
• RefSeq Size: 1618 bp
• RefSeq ORF: 978 bp
• Locus ID: 26240
• Domains: XAP5
• MW: 38.7 kDa
• Gene Summary: This gene contains an intronless ORF that arose from ancestral retroposition. The encoded protein is related to a plant protein that plays a role in the circadian clock. This gene is adjacent to a differentially methylated region (DMR) and is imprinted and paternally expressed in many tissues. [provided by RefSeq, Nov 2015]