SOX15 (NM_006942) Human Tagged ORF Clone

CAT#: RC201586

SOX15 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 15 (SOX15)

Product Images

Western blot validation of overexpression lysate (Cat# LY416305) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201586 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SOX15
• Synonyms: SOX20; SOX26; SOX27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201586 representing NM_006942
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTACCAGGCTCCTCACAGGACCAGGCCTGGAGCCTGGAGCCTCCGGCTGCCACGGCTGCTGCCT
  CCTCATCTTCGGGACCCCAGGAGCGGGAGGGCGCTGGGAGCCCCGCGGCCCCCGGGACGCTGCCCCTGGA
  GAAGGTGAAGCGGCCGATGAACGCGTTCATGGTGTGGAGCTCCGCTCAGCGCCGCCAGATGGCGCAGCAG
  AACCCCAAGATGCACAACTCCGAGATCTCCAAGCGCCTGGGCGCGCAGTGGAAGCTGCTGGACGAGGACG
  AGAAGCGGCCCTTCGTGGAGGAGGCCAAGCGGCTCCGCGCCCGACACCTGCGCGACTACCCCGACTACAA
  GTACCGGCCTCGGCGCAAGGCCAAGAGCTCGGGCGCCGGACCTTCCCGCTGCGGACAGGGAAGAGGCAAC
  CTGGCCAGCGGCGGCCCGCTCTGGGGGCCGGGGTACGCGACCACCCAACCGAGCAGAGGCTTTGGGTACA
  GACCCCCCAGCTACTCGACAGCCTACCTGCCTGGCAGCTATGGCTCTTCCCACTGCAAACTGGAAGCCCC
  CTCACCGTGCTCCCTCCCTCAGAGTGACCCTAGGCTCCAGGGGGAACTGCTGCCCACCTATACCCACTAC
  CTGCCCCCTGGCTCTCCCACTCCATACAACCCTCCCCTTGCTGGTGCCCCCATGCCCCTAACCCACCTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201586 representing NM_006942
  Red=Cloning site Green=Tags(s)
  
  MALPGSSQDQAWSLEPPAATAAASSSSGPQEREGAGSPAAPGTLPLEKVKRPMNAFMVWSSAQRRQMAQQ
  NPKMHNSEISKRLGAQWKLLDEDEKRPFVEEAKRLRARHLRDYPDYKYRPRRKAKSSGAGPSRCGQGRGN
  LASGGPLWGPGYATTQPSRGFGYRPPSYSTAYLPGSYGSSHCKLEAPSPCSLPQSDPRLQGELLPTYTHY
  LPPGSPTPYNPPLAGAPMPLTHL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006942
• ORF Size: 699 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006942.1, NP_008873.1
• RefSeq Size: 1394 bp
• RefSeq ORF: 702 bp
• Locus ID: 6665
• Cytogenetics: 17p13.1
• Protein Families: Transcription Factors
• MW: 25.1 kDa
• Gene Summary: 'This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. [provided by RefSeq, Jul 2008]'