ORC4L (ORC4) (NM_002552) Human Tagged ORF Clone
CAT#: RC201587
ORC4 (Myc-DDK-tagged)-Human origin recognition complex, subunit 4 (ORC4), transcript variant 2
"NM_002552" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ORC4 |
Synonyms | ORC4L; ORC4P |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201587 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGTCGTAAATCAAAGAGTAACAGCTTAATTCACACAGAGTGCCTTTCACAGGTACAAAGAATTT TACGTGAAAGATTTTGTCGTCAGAGTCCACATAGTAACCTATTTGGAGTGCAAGTACAATACAAACACTT AAGTGAGCTGCTGAAAAGAACTGCTCTCCATGGAGAGAGTAACTCTGTCCTTATTATCGGACCCCGAGGA TCAGGAAAAACTATGTTAATAAATCATGCTTTGAAAGAACTCATGGAAATAGAAGAAGTGAGTGAAAATG TATTACAAGTTCACTTAAATGGACTGCTGCAGATCAATGACAAAATCGCCCTAAAGGAAATCACAAGGCA GTTAAATCTGGAAAATGTAGTTGGAGATAAAGTTTTTGGAAGCTTTGCTGAAAACCTTTCATTTCTTCTG GAAGCTTTAAAAAAAGGTGACCGAACTAGCAGTTGCCCAGTGATCTTCATATTAGATGAATTTGATCTTT TTGCTCATCATAAAAACCAAACACTTCTCTATAATCTTTTTGACATTTCTCAGTCTGCACAGACCCCAAT AGCAGTTATTGGTCTTACATGTAGATTGGATATTTTGGAACTCTTAGAAAAAAGAGTGAAGTCAAGATTT TCTCACCGGCAGATACACTTAATGAATTCATTTGGTTTTCCACAGTATGTTAAAATATTTAAAGAACAGT TATCTCTACCTGCAGAGTTTCCAGACAAGGTTTTTGCTGAGAAGTGGAATGAAAATGTTCAGTATCTCTC AGAAGATAGAAGTGTGCAAGAAGTACTACAGAAGCATTTCAATATCAGCAAAAACCTGCGGTCATTACAC ATGCTATTGATGCTTGCTTTAAATCGAGTAACAGCATCGCACCCATTTATGACTGCCGTAGATCTAATGG AAGCAAGCCAACTGTGTAGCATGGACTCGAAAGCAAATATTGTACATGGTCTATCAGTCTTGGAAATCTG TCTTATAATAGCAATGAAACATTTAAATGACATCTATGAGGAAGAGCCATTTAATTTTCAAATGGTCTAT AATGAGTTTCAGAAGTTTGTTCAAAGGAAAGCACATTCCGTTTATAATTTTGAAAAACCTGTTGTCATGA AGGCTTTTGAACACTTGCAGCAATTAGAATTAATAAAGCCCATGGAAAGAACTTCAGGAAATTCACAGAG AGAGTACCAGCTGATGAAACTGCTTTTGGATAATACTCAAATTATGAATGCTCTGCAGAAATATCCCAAC TGTCCTACAGATGTGAGGCAGTGGGCAACATCCTCACTAAGCTGGTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201587 protein sequence
Red=Cloning site Green=Tags(s) MSSRKSKSNSLIHTECLSQVQRILRERFCRQSPHSNLFGVQVQYKHLSELLKRTALHGESNSVLIIGPRG SGKTMLINHALKELMEIEEVSENVLQVHLNGLLQINDKIALKEITRQLNLENVVGDKVFGSFAENLSFLL EALKKGDRTSSCPVIFILDEFDLFAHHKNQTLLYNLFDISQSAQTPIAVIGLTCRLDILELLEKRVKSRF SHRQIHLMNSFGFPQYVKIFKEQLSLPAEFPDKVFAEKWNENVQYLSEDRSVQEVLQKHFNISKNLRSLH MLLMLALNRVTASHPFMTAVDLMEASQLCSMDSKANIVHGLSVLEICLIIAMKHLNDIYEEEPFNFQMVY NEFQKFVQRKAHSVYNFEKPVVMKAFEHLQQLELIKPMERTSGNSQREYQLMKLLLDNTQIMNALQKYPN CPTDVRQWATSSLSWL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002552 |
ORF Size | 1308 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002552.1, NM_002552.2, NM_002552.3, NM_002552.4, NP_002543.2 |
RefSeq Size | 6594 bp |
RefSeq ORF | 1311 bp |
Locus ID | 5000 |
Cytogenetics | 2q23.1 |
Domains | AAA |
Protein Pathways | Cell cycle |
MW | 50.4 kDa |
Gene Summary | 'The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. [provided by RefSeq, Oct 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC109522 | ORC4 (untagged)-Human origin recognition complex, subunit 4 (ORC4), transcript variant 2 |
USD 310.00 |
|
SC322480 | ORC4 (untagged)-Human origin recognition complex, subunit 4 (ORC4), transcript variant 2 |
USD 310.00 |
|
RG201587 | ORC4 (GFP-tagged) - Human origin recognition complex, subunit 4 (ORC4), transcript variant 2 |
USD 460.00 |
|
RC201587L1 | Lenti ORF clone of Human origin recognition complex, subunit 4 (ORC4), transcript variant 2, Myc-DDK-tagged |
USD 768.00 |
|
RC201587L2 | Lenti ORF clone of Human origin recognition complex, subunit 4 (ORC4), transcript variant 2, mGFP tagged |
USD 620.00 |
|
RC201587L3 | Lenti ORF clone of Human origin recognition complex, subunit 4 (ORC4), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC201587L4 | Lenti ORF clone of Human origin recognition complex, subunit 4 (ORC4), transcript variant 2, mGFP tagged |
USD 620.00 |
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