ORC2 (NM_006190) Human Tagged ORF Clone

CAT#: RC201599

ORC2 (Myc-DDK-tagged)-Human origin recognition complex, subunit 2 (ORC2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY416811) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201599 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ORC2 protein (Cat# TP301599). The protein was produced from HEK293T cells transfected with ORC2 cDNA clone (Cat# RC201599) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORC2
• Synonyms: ORC2L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201599 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAACCAGAATTAAAGGAAGACAAGATGCTGGAGGTTCACTTTGTGGGAGATGATGATGTTCTTA
  ATCACATTCTAGATAGAGAAGGAGGAGCTAAATTGAAGAAGGAGCGAGCGCAGCTTTTGGTCAACCCCAA
  AAAAATAATAAAGAAGCCAGAATATGATTTGGAGGAAGATGACCAGGAGGTCTTAAAAGATCAGAACTAT
  GTGGAAATTATGGGAAGAGATGTTCAAGAATCATTGAAAAATGGCTCTGCTACAGGTGGTGGAAATAAAG
  TTTATTCTTTTCAGAATAGAAAACACTCTGAAAAGATGGCTAAATTAGCTTCAGAACTAGCAAAAACACC
  ACAAAAAAGTGTTTCATTCAGTTTGAAGAATGATCCTGAGATTACGATAAACGTTCCTCAAAGTAGCAAG
  GGCCATTCTGCTTCAGACAAGGTTCAACCGAAGAACAATGACAAAAGTGAATTTCTGTCAACAGCACCTC
  GTAGTCTAAGAAAAAGATTAATAGTTCCAAGGTCTCATTCTGACAGTGAAAGCGAATATTCTGCTTCCAA
  CTCAGAGGATGATGAAGGGGTTGCACAGGAACATGAAGAGGACACTAATGCAGTCATATTCAGCCAAAAG
  ATTCAAGCTCAGAATAGAGTAGTTTCAGCTCCTGTTGGCAAAGAAACACCTTCTAAGAGAATGAAAAGAG
  ATAAAACAAGTGACTTAGTAGAAGAATATTTTGAAGCTCACAGCAGTTCAAAAGTTTTAACCTCTGATAG
  AACACTGCAGAAGCTAAAGAGAGCTAAACTGGATCAGCAAACTTTGCGTAACTTATTGAGCAAGGTTTCC
  CCTTCCTTTTCTGCCGAACTTAAACAACTAAATCAACAGTATGAAAAATTATTTCATAAATGGATGCTGC
  AATTACACCTTGGGTTCAACATTGTGCTTTATGGTTTGGGTTCTAAGAGAGATTTACTAGAAAGGTTTCG
  AACCACTATGCTGCAAGATTCCATTCACGTTGTCATCAATGGCTTCTTTCCTGGAATCAGTGTGAAATCA
  GTCCTGAATTCTATAACAGAAGAAGTCCTCGATCATATGGGTACTTTCCGCAGTATACTGGATCAGCTAG
  ACTGGATAGTAAACAAATTTAAAGAAGATTCTTCTTTAGAACTCTTCCTTCTTATCCACAATTTGGATAG
  CCAGATGTTGAGAGGAGAGAAGAGCCAGCAAATCATTGGTCAGTTGTCATCTTTGCATAACATTTACCTT
  ATAGCATCCATTGACCACCTCAATGCTCCTCTCATGTGGGATCATGCAAAGCAGAGTCTTTTTAACTGGC
  TCTGGTATGAAACTACTACATACAGTCCTTATACTGAAGAAACCTCCTATGAGAACTCTCTTCTGGTAAA
  GCAGTCTGGATCCCTGCCACTTAGCTCCCTTACTCATGTCTTACGAAGCCTTACCCCTAATGCAAGGGGA
  ATTTTCAGGCTACTAATAAAATACCAGCTGGACAACCAGGATAACCCTTCTTACATTGGCCTTTCTTTTC
  AAGATTTTTACCAGCAGTGTCGGGAGGCATTCCTCGTCAATAGTGATCTGACACTCCGGGCCCAGTTAAC
  TGAATTTAGGGACCACAAGCTTATAAGAACAAAGAAGGGAACTGATGGAGTAGAGTATTTATTAATTCCT
  GTTGATAATGGAACATTGACTGATTTCTTGGAAAAGGAAGAAGAGGAGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201599 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSKPELKEDKMLEVHFVGDDDVLNHILDREGGAKLKKERAQLLVNPKKIIKKPEYDLEEDDQEVLKDQNY
  VEIMGRDVQESLKNGSATGGGNKVYSFQNRKHSEKMAKLASELAKTPQKSVSFSLKNDPEITINVPQSSK
  GHSASDKVQPKNNDKSEFLSTAPRSLRKRLIVPRSHSDSESEYSASNSEDDEGVAQEHEEDTNAVIFSQK
  IQAQNRVVSAPVGKETPSKRMKRDKTSDLVEEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVS
  PSFSAELKQLNQQYEKLFHKWMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKS
  VLNSITEEVLDHMGTFRSILDQLDWIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYL
  IASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLTHVLRSLTPNARG
  IFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEFRDHKLIRTKKGTDGVEYLLIP
  VDNGTLTDFLEKEEEEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006190
• ORF Size: 1731 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006190.1, NM_006190.2, NM_006190.3, NM_006190.4, NP_006181.1
• RefSeq Size: 3140 bp
• RefSeq ORF: 1734 bp
• Locus ID: 4999
• Cytogenetics: 2q33.1
• Domains: ORC2
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle
• MW: 66 kDa
• Gene Summary: 'The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate. [provided by RefSeq, Oct 2010]'