Adrenodoxin (FDX1) (NM_004109) Human Tagged ORF Clone
CAT#: RC201647
FDX1 (Myc-DDK-tagged)-Human ferredoxin 1 (FDX1), nuclear gene encoding mitochondrial protein
"NM_004109" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | FDX1 |
Synonyms | ADX; FDX; LOH11CR1D |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201647 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGCTGGGGGCGCCCGGCTGCTGCGCGCCGCTTCTGCTGTCCTCGGCGGCCCGGCCGGCCGGT GGCTGCACCACGCTGGGTCCCGCGCTGGATCCAGCGGCCTGCTGAGGAACCGGGGGCCGGGCGGGAGCGC GGAGGCGAGCCGGTCGCTGAGCGTGTCGGCGCGGGCCCGGAGCAGCTCAGAAGATAAAATAACAGTCCAC TTTATAAACCGTGATGGTGAAACATTAACAACCAAAGGAAAAGTTGGTGATTCTCTGCTAGATGTTGTGG TTGAAAATAATCTAGATATTGATGGCTTTGGTGCATGTGAGGGAACCCTGGCTTGTTCAACCTGTCACCT CATCTTTGAAGATCACATATATGAGAAGTTAGATGCAATCACTGATGAGGAGAATGACATGCTCGATCTG GCATATGGACTAACAGACAGATCACGGTTGGGCTGCCAAATCTGTTTGACAAAATCTATGGACAATATGA CTGTTCGAGTGCCTGAAACAGTGGCTGATGCCAGACAATCCATTGATGTGGGCAAGACCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201647 protein sequence
Red=Cloning site Green=Tags(s) MAAAGGARLLRAASAVLGGPAGRWLHHAGSRAGSSGLLRNRGPGGSAEASRSLSVSARARSSSEDKITVH FINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDL AYGLTDRSRLGCQICLTKSMDNMTVRVPETVADARQSIDVGKTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004109 |
ORF Size | 552 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004109.1, NM_004109.2, NM_004109.3, NM_004109.4, NP_004100.1 |
RefSeq Size | 3155 bp |
RefSeq ORF | 555 bp |
Locus ID | 2230 |
Cytogenetics | 11q22.3 |
Domains | fer2 |
MW | 19.4 kDa |
Gene Summary | 'This gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism. Pseudogenes of this functional gene are found on chromosomes 20 and 21. [provided by RefSeq, Aug 2011]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108419 | FDX1 (untagged)-Human ferredoxin 1 (FDX1), nuclear gene encoding mitochondrial protein |
USD 310.00 |
|
RG201647 | FDX1 (GFP-tagged) - Human ferredoxin 1 (FDX1), nuclear gene encoding mitochondrial protein |
USD 460.00 |
|
RC201647L1 | Lenti ORF clone of Human ferredoxin 1 (FDX1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
USD 768.00 |
|
RC201647L2 | Lenti ORF clone of Human ferredoxin 1 (FDX1), nuclear gene encoding mitochondrial protein, mGFP tagged |
USD 620.00 |
|
RC201647L3 | Lenti ORF clone of Human ferredoxin 1 (FDX1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
USD 620.00 |
|
RC201647L4 | Lenti ORF clone of Human ferredoxin 1 (FDX1), nuclear gene encoding mitochondrial protein, mGFP tagged |
USD 620.00 |
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