EIF3F (NM_003754) Human Tagged ORF Clone

CAT#: RC201740

EIF3F (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit F (EIF3F)

Product Images

Western blot validation of overexpression lysate (Cat# LY418450) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201740 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EIF3F protein (Cat# TP301740). The protein was produced from HEK293T cells transfected with EIF3F cDNA clone (Cat# RC201740) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF3F
• Synonyms: eIF3-p47; EIF3S5; MRT67
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201740 representing NM_003754
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACACCGGCGGTACCAGTAAGTGCTCCTCCGGCCACGCCAACCCCAGTCCCGGCGGCGGCCCCAG
  CCTCAGTTCCAGCGCCAACGCCAGCACCGGCTGCGGCTCCGGTTCCCGCTGCGGCTCCAGCCTCATCCTC
  AGACCCTGCGGCAGCAGCGGCTGCAACTGCGGCTCCTGGCCAGACCCCGGCCTCAGCGCAAGCTCCAGCG
  CAGACCCCAGCGCCCGCTCTGCCTGGTCCTGCTCTTCCAGGGCCCTTCCCCGGCGGCCGCGTGGTCAGGC
  TGCACCCAGTCATTTTGGCCTCCATTGTGGACAGCTACGAGAGACGCAACGAGGGTGCTGCCCGAGTTAT
  CGGGACCCTGTTGGGAACTGTCGACAAACACTCAGTGGAGGTCACCAATTGCTTTTCAGTGCCGCACAAT
  GAGTCAGAAGATGAAGTGGCTGTTGACATGGAATTTGCTAAGAATATGTATGAACTGCATAAAAAAGTTT
  CTCCAAATGAGCTCATCCTGGGCTGGTACGCTACGGGCCATGACATCACAGAGCACTCTGTGCTGATCCA
  TGAGTACTACAGCCGAGAGGCCCCCAACCCCATCCACCTCACTGTGGACACAAGTCTCCAGAACGGCCGC
  ATGAGCATCAAAGCCTACGTCAGCACTTTAATGGGAGTCCCTGGGAGGACCATGGGAGTGATGTTCACGC
  CTCTGACAGTGAAATACGCGTACTACGACACTGAACGCATCGGAGTTGACCTGATCATGAAGACCTGCTT
  TAGCCCCAACAGAGTGATTGGACTCTCAAGTGACTTGCAGCAAGTAGGAGGGGCATCAGCTCGCATCCAG
  GATGCCCTGAGTACAGTGTTGCAATATGCAGAGGATGTACTGTCTGGAAAGGTGTCAGCTGACAATACTG
  TGGGCCGCTTCCTGATGAGCCTGGTTAACCAAGTACCGAAAATAGTTCCCGATGACTTTGAGACCATGCT
  CAACAGCAACATCAATGACCTTTTGATGGTGACCTACCTGGCCAACCTCACACAGTCACAGATTGCACTC
  AATGAAAAACTTGTAAACCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201740 representing NM_003754
  Red=Cloning site Green=Tags(s)
  
  MATPAVPVSAPPATPTPVPAAAPASVPAPTPAPAAAPVPAAAPASSSDPAAAAAATAAPGQTPASAQAPA
  QTPAPALPGPALPGPFPGGRVVRLHPVILASIVDSYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHN
  ESEDEVAVDMEFAKNMYELHKKVSPNELILGWYATGHDITEHSVLIHEYYSREAPNPIHLTVDTSLQNGR
  MSIKAYVSTLMGVPGRTMGVMFTPLTVKYAYYDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQ
  DALSTVLQYAEDVLSGKVSADNTVGRFLMSLVNQVPKIVPDDFETMLNSNINDLLMVTYLANLTQSQIAL
  NEKLVNL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003754
• ORF Size: 1071 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003754.1, NM_003754.2, NP_003745.1
• RefSeq Size: 1274 bp
• RefSeq ORF: 1074 bp
• Locus ID: 8665
• Domains: JAB_MPN
• Protein Families: Druggable Genome
• MW: 37.4 kDa