EFTUD2 (NM_004247) Human Tagged ORF Clone

CAT#: RC201823

EFTUD2 (Myc-DDK-tagged)-Human elongation factor Tu GTP binding domain containing 2 (EFTUD2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418117) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201823 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EFTUD2 protein (Cat# TP301823). The protein was produced from HEK293T cells transfected with EFTUD2 cDNA clone (Cat# RC201823) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EFTUD2
• Synonyms: MFDGA; MFDM; SNRNP116; Snrp116; Snu114; U5-116KD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201823 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACCGACTTATATGATGAGTTTGGGAATTATATTGGACCAGAGCTTGATTCTGATGAAGATGATG
  ATGAATTGGGTAGAGAGACCAAAGATCTTGATGAGATGGATGATGATGACGACGACGATGACGTAGGAGA
  TCATGACGATGACCACCCTGGGATGGAGGTGGTGCTGCATGAGGACAAGAAGTACTACCCAACAGCCGAG
  GAGGTGTATGGTCCTGAGGTGGAGACCATAGTTCAAGAGGAAGACACTCAGCCTCTCACAGAACCCATTA
  TTAAGCCAGTGAAAACCAAGAAATTCACTCTGATGGAGCAGACATTACCTGTTACGGTGTATGAGATGGA
  TTTCTTGGCGGATCTGATGGATAACTCAGAGCTCATCAGAAATGTGACCCTTTGTGGACATCTCCACCAT
  GGCAAGACATGTTTTGTGGATTGTTTAATTGAACAGACTCACCCGGAAATCAGAAAGCGCTATGACCAAG
  ATCTGTGCTATACTGACATCCTCTTCACAGAGCAAGAGAGAGGTGTAGGCATCAAAAGCACTCCTGTGAC
  AGTGGTCTTGCCAGACACCAAAGGAAAATCTTATCTCTTCAATATCATGGACACTCCAGGACATGTGAAT
  TTCTCTGATGAGGTCACAGCTGGCTTGCGCATCTCAGATGGAGTGGTCCTTTTCATTGATGCTGCTGAGG
  GGGTGATGCTGAACACAGAGCGGCTGATCAAGCATGCGGTGCAGGAGAGGCTGGCAGTCACTGTGTGCAT
  CAACAAGATTGACCGGCTGATCCTGGAGCTGAAGCTGCCTCCAACTGATGCTTATTACAAGCTGCGCCAC
  ATTGTGGATGAGGTCAATGGATTAATAAGCATGTATTCCACTGATGAGAACCTGATCCTTTCCCCACTCC
  TGGGTAACGTCTGCTTCTCCAGCTCCCAGTACAGCATCTGCTTCACGCTGGTCTCCTTTGCCAAGATCTA
  TGCCGACACCTTTGGTGACATTAATTACCAAGAATTTGCTAAAAGACTCTGGGGTGACATCTACTTCAAC
  CCTAAGACGCGAAAGTTCACCAAAAAGGCCCCAACTAGCAGCTCCCAGAGAAGTTTCGTGGAGTTTATCT
  TGGAGCCTCTTTATAAGATCCTCGCCCAGGTTGTAGGTGACGTGGACACCAGCCTCCCACGGACCCTAGA
  CGAGCTTGGCATCCACCTGACGAAGGAGGAGCTGAAGCTGAACATCCGCCCCTTGCTCAGGCTGGTCTGC
  AAAAAGTTCTTTGGCGAGTTCACAGGCTTTGTGGACATGTGTGTGCAGCATATCCCTTCTCCAAAGGTGG
  GCGCCAAGCCCAAGATTGAGCACACCTACACCGGTGGTGTGGACTCCGACCTCGGCGAGGCTATGAGTGA
  CTGTGACCCTGATGGCCCCCTGATGTGCCACACTACTAAGATGTACAGCACAGATGATGGAGTCCAGTTT
  CACGCCTTTGGCCGGGTGCTGAGTGGCACCATTCATGCTGGGCAGCCTGTGAAGGTACTGGGGGAGAACT
  ACACCCTGGAGGATGAGGAAGACTCCCAGATATGCACCGTGGGCCGCCTTTGGATCTCTGTGGCCAGGTA
  CCACATCGAGGTGAACCGTGTTCCTGCTGGCAACTGGGTTCTGATTGAAGGTGTTGATCAACCAATTGTG
  AAGACAGCAACCATAACCGAACCCCGAGGCAATGAGGAGGCTCAGATTTTCCGACCCTTGAAGTTCAATA
  CCACATCTGTTATCAAGATTGCTGTGGAGCCAGTCAACCCCTCAGAGCTGCCCAAGATGCTTGATGGCCT
  GCGCAAGGTCAACAAGAGCTATCCATCCCTCACCACCAAGGTGGAGGAGTCTGGCGAGCATGTGATCCTG
  GGCACTGGGGAGCTCTACCTGGACTGTGTGATGCATGATTTGCGGAAGATGTACTCAGAGATAGACATCA
  AGGTGGCTGACCCAGTTGTCACGTTTTGTGAGACGGTGGTGGAAACATCCTCCCTCAAGTGCTTTGCTGA
  AACGCCTAATAAGAAGAACAAGATCACCATGATTGCTGAGCCTCTTGAGAAGGGCCTGGCAGAGGACATA
  GAGAATGAGGTGGTCCAGATTACGTGGAACAGGAAGAAGCTGGGAGAGTTCTTCCAGACCAAGTACGATT
  GGGATCTGCTGGCTGCCCGTTCCATCTGGGCTTTTGGCCCTGATGCGACTGGCCCCAACATTCTGGTGGA
  TGATACTCTGCCCTCTGAGGTGGACAAGGCTCTTCTTGGTTCAGTGAAGGACAGCATCGTTCAAGGTTTC
  CAGTGGGGAACCAGGGAGGGCCCCCTCTGTGATGAATTGATTCGGAATGTCAAGTTTAAGATCCTGGATG
  CGGTGGTTGCCCAGGAGCCCCTGCACCGGGGCGGGGGCCAGATCATCCCCACAGCCAGGAGAGTCGTCTA
  CTCTGCCTTCCTCATGGCTACTCCTCGTCTGATGGAGCCTTACTACTTTGTAGAGGTCCAGGCCCCTGCA
  GATTGCGTCTCTGCAGTTTATACCGTCCTGGCCAGGCGCAGGGGGCACGTGACTCAGGATGCACCCATCC
  CAGGCTCCCCTCTGTACACCATCAAAGCTTTTATCCCGGCCATCGACTCTTTTGGCTTTGAGACTGATCT
  CCGGACTCACACCCAGGGACAAGCCTTTTCTCTGTCTGTCTTCCACCACTGGCAGATTGTGCCTGGTGAT
  CCCCTGGACAAGAGCATTGTCATCCGCCCCTTGGAGCCACAGCCAGCTCCTCACCTGGCCCGGGAATTCA
  TGATCAAAACCCGCCGTAGGAAGGGCCTCAGTGAAGATGTGAGCATCAGCAAATTCTTCGATGATCCTAT
  GTTGCTGGAACTTGCCAAACAGGATGTTGTGCTCAATTACCCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201823 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAE
  EVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHH
  GKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVN
  FSDEVTAGLRISDGVVLFIDAAEGVMLNTERLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRH
  IVDEVNGLISMYSTDENLILSPLLGNVCFSSSQYSICFTLVSFAKIYADTFGDINYQEFAKRLWGDIYFN
  PKTRKFTKKAPTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
  KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTKMYSTDDGVQF
  HAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIEVNRVPAGNWVLIEGVDQPIV
  KTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVIL
  GTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDI
  ENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGF
  QWGTREGPLCDELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
  DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSVFHHWQIVPGD
  PLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVVLNYPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004247
• ORF Size: 2916 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004247.1, NM_004247.2, NM_004247.3, NP_004238.2
• RefSeq Size: 4167 bp
• RefSeq ORF: 2919 bp
• Locus ID: 9343
• Domains: EFG_C, GTP_EFTU, GTP_EFTU_D2, EFG_IV
• Protein Pathways: Spliceosome
• MW: 109.5 kDa
• Gene Summary: This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]