KIAA0652 (ATG13) (NM_014741) Human Tagged ORF Clone

CAT#: RC201825

ATG13 (Myc-DDK-tagged)-Human ATG13 autophagy related 13 homolog (S. cerevisiae) (ATG13), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY415059) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201825 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATG13
• Synonyms: KIAA0652; PARATARG8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201825 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTGATCTCAATTCCCAGGACAGAAAGGACCTGGACAAGTTTATTAAATTTTTTGCCCTCAAGA
  CTGTCCAAGTGATTGTCCAGGCTCGGCTTGGTGAAAAGATTTGCACTCGTTCATCATCTTCTCCAACGGG
  TTCAGATTGGTTCAACTTAGCAATCAAAGACATCCCAGAGGTTACACATGAAGCAAAGAAGGCACTGGCA
  GGACAGCTGCCTGCAGTCGGGAGGTCCATGTGTGTGGAGATTTCACTTAAGACTTCTGAGGGAGATTCCA
  TGGAGCTGGAAATATGGTGTCTTGAAATGAATGAAAAGTGTGATAAAGAAATCAAAGTTTCCTACACGGT
  GTACAACAGACTGTCATTGCTGCTGAAGTCCCTTCTTGCTATAACTAGGGTGACACCAGCCTATAGGCTC
  TCCAGGAAACAAGGGCATGAATATGTCATATTATACAGGATATATTTTGGAGAAGTTCAGCTGAGTGGCT
  TAGGAGAAGGCTTCCAGACAGTTCGTGTTGGGACAGTGGGCACCCCTGTGGGCACCATCACTCTTTCTTG
  TGCTTACAGAATTAACTTGGCATTCATGTCTACCAGGCAATTTGAGAGGACCCCACCTATCATGGGGATT
  ATTATTGATCACTTTGTGGACCGTCCCTATCCCAGCTCCTCTCCCATGCACCCCTGCAATTACAGAACTG
  CTGGTGAGGACACTGGAGTAATATACCCGTCTGTAGAAGACTCTCAAGAAGTGTGTACCACCTCTTTTTC
  CACCTCCCCACCATCCCAGCTGATGGTTCCTGGGAAGGAAGGTGGGGTACCCCTTGCTCCCAACCAGCCT
  GTCCATGGTACCCAGGCTGACCAGGAGAGACTGGCAACCTGCACCCCTTCTGACAGAACCCACTGTGCTG
  CCACACCCTCCAGTAGTGAGGATACTGAAACCGTATCAAACAGCAGTGAGGGACGGGCCTCCCCTCACGA
  TGTCTTGGAGACCATCTTTGTCCGAAAAGTGGGGGCTTTTGTCAACAAACCCATTAACCAGGTGACCCTG
  ACGAGTTTGGATATACCCTTTGCCATGTTTGCTCCCAAGAATTTGGAGCTGGAGGATACCGATCCAATGG
  TGAATCCTCCAGATTCCCCAGAGACTGAATCTCCTCTCCAGGGCAGCCTGCACTCAGATGGCTCCAGCGG
  GGGCAGCAGTGGCAATACCCATGATGACTTTGTTATGATAGACTTTAAACCAGCTTTTTCTAAAGATGAC
  ATTCTTCCGATGGACCTGGGGACCTTCTATCGGGAGTTTCAGAACCCACCTCAGCTGAGCAGCCTCTCCA
  TAGATATTGGAGCACAGTCCATGGCTGAAGACTTGGACTCATTACCAGAGAAGCTGGCTGTGCATGAGAA
  GAATGTCCGCGAGTTTGATGCCTTTGTGGAAACCCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201825 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METDLNSQDRKDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDWFNLAIKDIPEVTHEAKKALA
  GQLPAVGRSMCVEISLKTSEGDSMELEIWCLEMNEKCDKEIKVSYTVYNRLSLLLKSLLAITRVTPAYRL
  SRKQGHEYVILYRIYFGEVQLSGLGEGFQTVRVGTVGTPVGTITLSCAYRINLAFMSTRQFERTPPIMGI
  IIDHFVDRPYPSSSPMHPCNYRTAGEDTGVIYPSVEDSQEVCTTSFSTSPPSQLMVPGKEGGVPLAPNQP
  VHGTQADQERLATCTPSDRTHCAATPSSSEDTETVSNSSEGRASPHDVLETIFVRKVGAFVNKPINQVTL
  TSLDIPFAMFAPKNLELEDTDPMVNPPDSPETESPLQGSLHSDGSSGGSSGNTHDDFVMIDFKPAFSKDD
  ILPMDLGTFYREFQNPPQLSSLSIDIGAQSMAEDLDSLPEKLAVHEKNVREFDAFVETLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014741
• ORF Size: 1440 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014741.2, NM_014741.3, NM_014741.4, NP_055556.2
• RefSeq Size: 5812 bp
• RefSeq ORF: 1443 bp
• Locus ID: 9776
• MW: 52.8 kDa
• Gene Summary: The protein encoded by this gene is an autophagy factor and a target of the TOR kinase signaling pathway. The encoded protein is essential for autophagosome formation and mitophagy. [provided by RefSeq, Oct 2016]