ASC1 (TRIP4) (NM_016213) Human Tagged ORF Clone

CAT#: RC201863

TRIP4 (Myc-DDK-tagged)-Human thyroid hormone receptor interactor 4 (TRIP4)

Product Images

Western blot validation of overexpression lysate (Cat# LY414127) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201863 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIP4
• Synonyms: ASC-1; ASC1; HsT17391; MDCDC; SMABF1; ZC2HC5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201863 representing NM_016213
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGGCTGGGGCGGTGTCCGGGGAGCCGCTGGTGCACTGGTGCACCCAGCAGTTGCGGAAGACTT
  TCGGCCTGGATGTCAGCGAGGAGATCATTCAGTACGTTTTGTCAATTGAGAGTGCTGAAGAGATACGAGA
  ATATGTTACTGATCTCCTCCAGGGAAATGAAGGCAAAAAAGGTCAATTCATAGAAGAACTTATAACCAAA
  TGGCAAAAGAATGATCAGGAGTTGATTTCGGATCCTTTGCAGCAGTGCTTCAAAAAAGATGAAATTTTAG
  ATGGGCAGAAATCAGGCGACCATCTAAAGCGGGGTAGGAAGAAAGGGAGAAACAGACAGGAAGTTCCTGC
  ATTTACTGAACCTGACACGACTGCAGAGGTTAAAACACCTTTTGATTTGGCCAAGGCACAAGAGAACAGC
  AACTCCGTAAAGAAGAAGACAAAGTTTGTCAATTTATACACAAGAGAGGGACAGGACAGGCTTGCAGTCC
  TGCTCCCTGGTCGTCACCCTTGTGATTGCCTGGGCCAGAAGCACAAGCTCATCAATAACTGTCTGATCTG
  TGGGCGCATTGTCTGTGAACAAGAAGGCTCAGGCCCTTGCTTATTCTGTGGCACTCTGGTGTGTACTCAT
  GAGGAACAAGATATTTTACAGCGTGACTCAAACAAGAGCCAGAAACTGCTAAAGAAACTCATGTCAGGAG
  TGGAGAATTCTGGAAAGGTGGACATCTCTACCAAGGACCTTCTTCCTCATCAAGAATTGCGAATTAAGTC
  TGGTCTGGAGAAGGCTATCAAGCATAAAGACAAACTGTTAGAGTTTGACAGAACTAGTATTCGAAGGACC
  CAAGTCATTGATGATGAGTCAGATTACTTTGCCAGTGATTCTAACCAATGGTTGTCCAAACTTGAGCGGG
  AAACCTTGCAGAAGCGAGAGGAGGAGCTGAGAGAACTTCGACACGCCTCTCGACTTTCTAAGAAGGTCAC
  CATTGACTTTGCAGGAAGGAAGATCCTGGAAGAAGAAAATTCACTAGCAGAGTATCATAGCAGACTAGAT
  GAGACAATACAGGCCATTGCCAATGGAACCTTGAACCAGCCACTGACCAAATTGGATAGATCTTCTGAAG
  AGCCTTTGGGAGTTCTGGTAAATCCCAACATGTACCAGTCCCCTCCCCAGTGGGTTGACCACACAGGTGC
  AGCCTCACAGAAGAAGGCTTTCCGTTCTTCAGGATTTGGACTAGAGTTCAACTCATTTCAGCACCAGCTG
  CGAATCCAGGATCAAGAATTTCAGGAAGGCTTTGATGGTGGCTGGTGCCTCTCTGTACATCAGCCCTGGG
  CTTCTCTGCTTGTCAGAGGGATTAAAAGGGTGGAGGGCAGATCCTGGTACACCCCCCACAGAGGACGACT
  TTGGATAGCAGCCACAGCTAAAAAACCCTCCCCTCAAGAAGTCTCAGAACTCCAGGCTACATATCGTCTT
  CTTCGTGGGAAAGATGTGGAATTTCCTAATGACTATCCGTCAGGTTGTCTTCTGGGCTGTGTGGACCTAA
  TTGACTGCTTGTCCCAGAAGCAATTTAAGGAGCAGTTTCCAGACATCAGTCAAGAATCTGATTCTCCATT
  TGTTTTCATCTGCAAAAATCCTCAGGAAATGGTTGTGAAGTTTCCTATTAAAGGAAATCCAAAAATCTGG
  AAATTGGATTCCAAGATCCATCAAGGAGCAAAGAAGGGGTTAATGAAGCAGAATAAAGCTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201863 representing NM_016213
  Red=Cloning site Green=Tags(s)
  
  MAVAGAVSGEPLVHWCTQQLRKTFGLDVSEEIIQYVLSIESAEEIREYVTDLLQGNEGKKGQFIEELITK
  WQKNDQELISDPLQQCFKKDEILDGQKSGDHLKRGRKKGRNRQEVPAFTEPDTTAEVKTPFDLAKAQENS
  NSVKKKTKFVNLYTREGQDRLAVLLPGRHPCDCLGQKHKLINNCLICGRIVCEQEGSGPCLFCGTLVCTH
  EEQDILQRDSNKSQKLLKKLMSGVENSGKVDISTKDLLPHQELRIKSGLEKAIKHKDKLLEFDRTSIRRT
  QVIDDESDYFASDSNQWLSKLERETLQKREEELRELRHASRLSKKVTIDFAGRKILEEENSLAEYHSRLD
  ETIQAIANGTLNQPLTKLDRSSEEPLGVLVNPNMYQSPPQWVDHTGAASQKKAFRSSGFGLEFNSFQHQL
  RIQDQEFQEGFDGGWCLSVHQPWASLLVRGIKRVEGRSWYTPHRGRLWIAATAKKPSPQEVSELQATYRL
  LRGKDVEFPNDYPSGCLLGCVDLIDCLSQKQFKEQFPDISQESDSPFVFICKNPQEMVVKFPIKGNPKIW
  KLDSKIHQGAKKGLMKQNKAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016213
• ORF Size: 1743 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016213.1, NM_016213.2, NM_016213.3, NM_016213.4, NP_057297.2
• RefSeq Size: 2044
• RefSeq ORF: 1746
• Locus ID: 9325
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• MW: 66 kDa
• Gene Summary: This gene encodes a subunit of the tetrameric nuclear activating signal cointegrator 1 (ASC-1) complex, which associates with transcriptional coactivators, nuclear receptors and basal transcription factors to facilitate nuclear receptors-mediated transcription. This protein is localized in the nucleus and contains an E1A-type zinc finger domain, which mediates interaction with transcriptional coactivators and ligand-bound nuclear receptors, such as thyroid hormone receptor and retinoid X receptor alpha, but not glucocorticoid receptor. Mutations in this gene are associated with spinal muscular atrophy with congenital bone fractures-1 (SMABF1). [provided by RefSeq, Apr 2016]