TARS (NM_152295) Human Tagged ORF Clone

CAT#: RC202140

TARS (Myc-DDK-tagged)-Human threonyl-tRNA synthetase (TARS)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TARS
• Synonyms: ThrRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202140 representing NM_152295
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGAGGAGAAGGCCAGCAGTCCTTCAGGGAAGATGGGAGGCGAGGAGAAGCCGATTGGTGCTGGTG
  AAGAGAAGCAAAAGGAAGGAGGCAAAAAGAAGAACAAAGAAGGATCTGGAGATGGAGGTCGAGCTGAGTT
  GAATCCTTGGCCTGAATATATTTACACACGTCTTGAGATGTATAATATACTAAAAGCAGAACATGATTCC
  ATTCTGGCAGAAAAGGCAGAAAAAGATAGCAAGCCAATTAAAGTCACTTTGCCTGATGGTAAACAGGTTG
  ATGCGGAATCTTGGAAAACTACACCATATCAAATTGCCTGTGGAATTAGTCAAGGCCTGGCCGACAACAC
  CGTTATTGCTAAAGTAAATAATGTTGTGTGGGACCTGGACCGCCCTCTGGAAGAAGATTGTACCTTGGAG
  CTTCTCAAGTTTGAGGATGAGGAAGCTCAGGCAGTGTATTGGCACTCTAGTGCTCACATAATGGGTGAAG
  CCATGGAAAGAGTCTATGGTGGATGTTTATGCTACGGTCCGCCAATAGAAAATGGATTCTATTATGACAT
  GTACCTCGAAGAAGGGGGTGTGTCTAGCAATGATTTCTCTTCTCTGGAGGCTTTGTGTAAGAAAATCATT
  AAAGAAAAACAAGCTTTTGAAAGACTGGAAGTTAAGAAAGAAACTTTACTGGCAATGTTTAAGTACAACA
  AGTTCAAATGCCGGATATTGAATGAAAAGGTGAATACTCCAACTACCACAGTCTATAGATGTGGCCCTTT
  GATAGATCTCTGCCGGGGTCCTCATGTTAGACACACGGGCAAAATTAAGGCTTTAAAAATACACAAAAAT
  TCCTCCACGTACTGGGAAGGCAAAGCAGATATGGAGACTCTCCAGAGAATTTATGGCATTTCATTCCCAG
  ATCCTAAAATGTTGAAAGAGTGGGAGAAGTTCCAAGAGGAAGCTAAAAACCGAGATCATAGGAAAATTGG
  CAGGGACCAAGAACTATATTTCTTTCATGAACTCAGCCCTGGAAGTTGCTTTTTTCTGCCAAAAGGAGCC
  TACATTTATAATGCACTTATTGAATTCATTAGGAGCGAATATAGGAAAAGAGGATTCCAGGAGGTAGTCA
  CCCCAAACATCTTCAACAGCCGACTCTGGATGACCTCGGGCCACTGGCAGCACTACAGCGAGAACATGTT
  CTCCTTTGAGGTGGAGAAGGAGCTGTTTGCCCTGAAACCCATGAACTGCCCAGGACACTGCCTTATGTTT
  GATCATCGGCCAAGGTCCTGGCGAGAACTGCCTCTGCGGCTAGCTGATTTTGGGGTACTTCATAGGAACG
  AGCTGTCTGGAGCACTCACAGGACTCACCCGGGTACGAAGATTCCAACAGGATGATGCTCACATATTCTG
  TGCCATGGAGCAGATTGAAGATGAAATAAAAGGTTGTTTGGATTTTCTACGTACGGTATATAGCGTATTT
  GGATTTTCTTTTAAACTAAACCTTTCTACTCGCCCGGAAAAATTCCTTGGAGATATCGAAGTATGGGATC
  AAGCTGAGAAACAACTTGAAAACAGTCTGAATGAATTTGGTGAAAAGTGGGAGTTAAACTCTGGAGATGG
  AGCTTTCTATGGCCCAAAGATTGACATACAGATTAAAGATGCGATTGGGCGGTACCACCAGTGTGCAACC
  ATCCAGCTGGATTTCCAGTTGCCCATCAGATTTAATCTTACTTATGTAAGCCATGATGGTGATGATAAGA
  AAAGGCCAGTGATTGTTCATCGAGCCATCTTGGGATCAGTGGAAAGAATGATTGCTATCCTCACAGAAAA
  CTATGGGGGCAAATGGCCCTTTTGGCTGTCCCCTCGCCAGGTAATGGTAGTTCCAGTGGGACCAACCTGT
  GATGAATATGCCCAAAAGGTACGACAACAATTCCACGATGCCAAATTCATGGCAGACATTGATCTGGATC
  CAGGCTGTACATTGAATAAAAAGATTCGAAATGCACAGTTAGCACAGTATAACTTCATTTTAGTTGTTGG
  TGAAAAAGAGAAAATCAGTGGCACTGTTAATATCCGCACAAGAGACAATAAGGTCCACGGGGAACGCACC
  ATTTCTGAAACTATCGAGCGGCTACAGCAGCTCAAAGAGTTCCGCAGCAAACAGGCAGAAGAAGAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202140 representing NM_152295
  Red=Cloning site Green=Tags(s)
  
  MFEEKASSPSGKMGGEEKPIGAGEEKQKEGGKKKNKEGSGDGGRAELNPWPEYIYTRLEMYNILKAEHDS
  ILAEKAEKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLE
  LLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLEALCKKII
  KEKQAFERLEVKKETLLAMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKIHKN
  SSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGA
  YIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVEKELFALKPMNCPGHCLMF
  DHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVF
  GFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCAT
  IQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTC
  DEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERT
  ISETIERLQQLKEFRSKQAEEEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152295
• ORF Size: 2169 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152295.3, NM_152295.4, NP_689508.3
• RefSeq Size: 2888 bp
• RefSeq ORF: 2172 bp
• Locus ID: 6897
• Cytogenetics: 5p13.3
• Domains: tRNA-synt_2b, TGS, HGTP_anticodon
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 105.8 kDa
• Gene Summary: 'Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase belongs to the class-II aminoacyl-tRNA synthetase family [provided by RefSeq, Jul 2008]'