Syntaxin 8 (STX8) (NM_004853) Human Tagged ORF Clone

CAT#: RC202177

STX8 (Myc-DDK-tagged)-Human syntaxin 8 (STX8), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417700) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202177 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STX8
• Synonyms: CARB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202177 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCGGACCCCTGGTTCTCCACATACGATTCTACTTGTCAAATTGCCCAAGAAATTGCTGAGAAAA
  TTCAACAACGAAATCAATATGAACGAAAAGGTGAAAAGGCACCAAAGCTTACCGTGACAATCAGAGCTTT
  GTTGCAGAACCTGAAGGAAAAGATCGCCCTTTTGAAGGACTTATTGCTAAGAGCTGTGTCAACACATCAG
  ATAACACAGCTTGAAGGGGACCGAAGACAGAACCTCTTGGATGATCTTGTAACTCGAGAGAGACTACTTC
  TGGCATCCTTTAAGAATGAGGGTGCCGAACCAGATCTAATCAGGTCCAGCCTGATGAGTGAAGAGGCTAA
  GCGAGGAGCACCCAACCCTTGGCTCTTTGAGGAGCCAGAGGAGACCAGAGGCTTGGGTTTTGATGAAATC
  CGGCAACAGCAGCAGAAAATTATCCAAGAACAGGATGCAGGCCTTGATGCCCTTTCCTCTATCATAAGTC
  GCCAAAAACAAATGGGGCAGGAAATTGGGAATGAATTGGATGAACAAAATGAGATAATTGACGACCTTGC
  CAACCTAGTGGAGAACACAGATGAAAAACTTCGCAATGAAACCAGGCGGGTAAACATGGTGGACAGAAAG
  TCAGCCTCTTGTGGGATGATCATGGTGATTTTACTGCTGCTTGTGGCTATCGTGGTTGTTGCAGTCTGGC
  CGACCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202177 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPDPWFSTYDSTCQIAQEIAEKIQQRNQYERKGEKAPKLTVTIRALLQNLKEKIALLKDLLLRAVSTHQ
  ITQLEGDRRQNLLDDLVTRERLLLASFKNEGAEPDLIRSSLMSEEAKRGAPNPWLFEEPEETRGLGFDEI
  RQQQQKIIQEQDAGLDALSSIISRQKQMGQEIGNELDEQNEIIDDLANLVENTDEKLRNETRRVNMVDRK
  SASCGMIMVILLLLVAIVVVAVWPTN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004853
• ORF Size: 708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004853.1, NM_004853.2, NP_004844.1
• RefSeq Size: 988 bp
• RefSeq ORF: 711 bp
• Locus ID: 9482
• Domains: t_SNARE
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• MW: 26.9 kDa
• Gene Summary: The gene is a member of the syntaxin family. The encoded protein is involved in protein trafficking from early to late endosomes via vesicle fusion and exocytosis. A related pseudogene has been identified on chromosome 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]