FKBP25 (FKBP3) (NM_002013) Human Tagged ORF Clone

CAT#: RC202200

FKBP3 (Myc-DDK-tagged)-Human FK506 binding protein 3, 25kDa (FKBP3)

Product Images

Western blot validation of overexpression lysate (Cat# LY400736) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202200 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FKBP3 protein (Cat# TP302200). The protein was produced from HEK293T cells transfected with FKBP3 cDNA clone (Cat# RC202200) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FKBP3
• Synonyms: FKBP-3; FKBP-25; FKBP25; PPIase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202200 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCCGTTCCACAGCGGGCGTGGACCGTGGAGCAGCTGCGCAGTGAGCAGCTGCCCAAGAAGG
  ACATTATCAAGTTTCTGCAGGAACACGGTTCAGATTCGTTTCTTGCAGAACATAAATTATTAGGAAACAT
  TAAAAATGTGGCCAAGACAGCTAACAAGGACCACTTGGTTACAGCCTATAACCATCTTTTTGAAACTAAG
  CGTTTTAAGGGTACTGAAAGTATAAGTAAAGTGTCTGAGCAAGTAAAAAATGTGAAGCTTAATGAAGATA
  AACCCAAAGAAACCAAGTCTGAAGAGACCCTGGATGAGGGTCCACCAAAATATACTAAATCTGTTCTGAA
  AAAGGGAGATAAAACCAACTTTCCCAAAAAGGGAGATGTTGTTCACTGCTGGTATACAGGAACACTACAA
  GATGGGACTGTTTTTGATACTAATATTCAAACAAGTGCAAAGAAGAAGAAAAATGCCAAGCCTTTAAGTT
  TTAAGGTCGGAGTAGGCAAAGTTATCAGAGGATGGGATGAAGCTCTCTTGACTATGAGTAAAGGAGAAAA
  GGCTCGACTGGAGATTGAACCAGAATGGGCTTACGGAAAGAAAGGACAGCCTGATGCCAAAATTCCACCA
  AATGCAAAACTCACTTTTGAAGTGGAATTAGTGGATATTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202200 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAVPQRAWTVEQLRSEQLPKKDIIKFLQEHGSDSFLAEHKLLGNIKNVAKTANKDHLVTAYNHLFETK
  RFKGTESISKVSEQVKNVKLNEDKPKETKSEETLDEGPPKYTKSVLKKGDKTNFPKKGDVVHCWYTGTLQ
  DGTVFDTNIQTSAKKKKNAKPLSFKVGVGKVIRGWDEALLTMSKGEKARLEIEPEWAYGKKGQPDAKIPP
  NAKLTFEVELVDID
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002013
• ORF Size: 672 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002013.1, NM_002013.2, NM_002013.3, NP_002004.1
• RefSeq Size: 1353 bp
• RefSeq ORF: 675 bp
• Locus ID: 2287
• Cytogenetics: 14q21.2
• Domains: FKBP
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 25.2 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin. [provided by RefSeq, Sep 2008]'