ARMC8 (NM_014154) Human Tagged ORF Clone

CAT#: RC202341

ARMC8 (Myc-DDK-tagged)-Human armadillo repeat containing 8 (ARMC8), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402281) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202341 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ARMC8 protein (Cat# TP302341). The protein was produced from HEK293T cells transfected with ARMC8 cDNA clone (Cat# RC202341) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARMC8
• Synonyms: GID5; HSPC056; S863-2; VID28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202341 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTAACAGCTAGCAGTCGCCACTATGTTGACAGGCTATTTGACCCTGATCCCCAGAAAGTTCTAC
  AAGGTGTCATAGACATGAAAAATGCTGTAATTGGAAACAACAAGCAGAAAGCCAATCTCATTGTTTTAGG
  AGCTGTTCCAAGATTGTTGTACTTGCTTCAGCAAGAAACCTCAAGCACAGAGCTGAAAACTGAATGTGCA
  GTGGTGTTGGGAAGTCTTGCTATGGGTACTGAAAACAATGTCAAGTCTCTACTGGACTGCCATATTATCC
  CTGCCTTATTGCAAGGACTACTGTCCCCAGACCTGAAGTTTATTGAAGCTTGCCTCCGATGCCTGCGTAC
  CATCTTCACCAGTCCTGTCACTCCAGAGGAGCTACTGTATACAGATGCCACAGTGATACCACACCTCATG
  GCACTGCTTAGCAGGTCCCGCTATACCCAGGAGTACATCTGTCAGATCTTCTCACACTGCTGTAAAGGGC
  CAGATCATCAAACAATTTTATTTAACCACGGTGCAGTTCAGAATATTGCTCACCTACTAACCTCACTGTC
  CTACAAAGTTCGAATGCAAGCACTGAAATGTTTCTCAGTTTTAGCTTTTGAAAACCCCCAGGTATCGATG
  ACCCTGGTAAATGTTTTGGTTGATGGAGAATTGTTACCACAGATTTTTGTGAAGATGTTACAGAGGGATA
  AGCCTATTGAGATGCAGCTCACATCAGCAAAATGTTTAACTTACATGTGTAGAGCTGGAGCAATTCGGAC
  AGATGATAACTGTATTGTATTAAAGACATTACCTTGTTTGGTTCGAATGTGCAGTAAGGAGAGATTACTA
  GAGGAGAGAGTTGAAGGAGCTGAGACACTTGCCTATCTGATTGAACCAGATGTTGAGCTACAGAGAATCG
  CTAGCATAACTGATCACCTCATTGCCATGCTTGCTGATTATTTCAAGTATCCCAGCTCAGTGAGTGCCAT
  CACTGATATTAAAAGGCTTGATCATGATTTAAAACATGCTCACGAACTCCGCCAGGCTGCATTCAAGCTC
  TATGCCTCTCTTGGAGCAAATGATGAAGACATCCGGAAGAAGGTGAGTCTGGGAGAGGGGCGTCCCCCAG
  TCCTGACAGCCAGCAGGCAGGGAGTGACGTCAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202341 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVTASSRHYVDRLFDPDPQKVLQGVIDMKNAVIGNNKQKANLIVLGAVPRLLYLLQQETSSTELKTECA
  VVLGSLAMGTENNVKSLLDCHIIPALLQGLLSPDLKFIEACLRCLRTIFTSPVTPEELLYTDATVIPHLM
  ALLSRSRYTQEYICQIFSHCCKGPDHQTILFNHGAVQNIAHLLTSLSYKVRMQALKCFSVLAFENPQVSM
  TLVNVLVDGELLPQIFVKMLQRDKPIEMQLTSAKCLTYMCRAGAIRTDDNCIVLKTLPCLVRMCSKERLL
  EERVEGAETLAYLIEPDVELQRIASITDHLIAMLADYFKYPSSVSAITDIKRLDHDLKHAHELRQAAFKL
  YASLGANDEDIRKKVSLGEGRPPVLTASRQGVTST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014154
• ORF Size: 1155 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014154.2, NM_014154.3, NM_014154.4, NP_054873.2
• RefSeq Size: 3236 bp
• RefSeq ORF: 1158 bp
• Locus ID: 25852
• Domains: Armadillo_seg
• MW: 43 kDa