CLINT1 (NM_014666) Human Tagged ORF Clone

CAT#: RC202387

CLINT1 (Myc-DDK-tagged)-Human clathrin interactor 1 (CLINT1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY402360) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202387 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLINT1
• Synonyms: CLINT; ENTH; EPN4; EPNR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202387 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAACATGTGGAAGGTGCGCGAGCTGGTGGACAAAGCCACCAATGTTGTTATGAATTATTCAGAGA
  TCGAGTCTAAGGTTCGAGAGGCAACGAACGATGATCCTTGGGGACCTTCTGGGCAACTCATGGGAGAGAT
  TGCCAAGGCTACATTTATGTATGAACAATTTCCAGAACTTATGAACATGCTTTGGTCACGAATGTTAAAA
  GACAACAAAAAGAATTGGAGAAGAGTTTATAAGTCGTTGCTGCTCCTAGCTTACCTCATAAGGAATGGAT
  CAGAGCGTGTTGTTACAAGTGCCAGAGAACACATTTATGATTTACGATCCCTGGAAAATTACCACTTTGT
  AGATGAGCATGGTAAGGATCAAGGTATAAATATTCGACAGAAGGTGAAGGAATTGGTTGAATTTGCCCAG
  GATGACGACAGGCTTCGTGAAGAGCGAAAGAAAGCAAAGAAGAACAAAGACAAGTATGTTGGGGTTTCCT
  CAGACAGTGTTGGAGGATTCAGATACAGTGAAAGATATGATCCTGAGCCCAAATCAAAATGGGATGAGGA
  GTGGGATAAAAACAAGAGTGCTTTTCCATTCAGTGATAAATTAGGTGAGCTGAGTGATAAAATTGGAAGC
  ACAATTGATGACACCATCAGCAAGTTCCGGAGGAAAGATAGAGAAGACTCTCCAGAAAGATGCAGCGACA
  GCGATGAGGAAAAGAAAGCGAGAAGAGGCAGATCTCCCAAAGGTGAATTCAAAGATGAAGAGGAGACTGT
  GACGACAAAGCATATTCATATCACACAGGCCACAGAGACCACCACAACCAGACACAAGCGCACAGCAAAT
  CCTTCCAAAACCATTGATCTTGGAGCAGCAGCACATTACACAGGGGACAAAGCAAGTCCAGATCAGAATG
  CTTCAACCCACACACCTCAGTCTTCAGTTAAGACTTCAGTGCCTAGCAGCAAGTCATCTGGTGACCTTGT
  TGATCTGTTTGATGGCACCAGCCAGTCAACAGGAGGATCAGCTGATTTATTCGGAGGATTTGCTGACTTT
  GGCTCAGCTGCTGCATCAGGCAGTTTCCCTTCCCAAGTAACAGCAACAAGTGGGAATGGAGACTTTGGTG
  ACTGGAGTGCCTTCAACCAAGCCCCATCAGGCCCTGTTGCTTCCAGTGGCGAGTTCTTTGGCAGTGCCTC
  ACAGCCAGCGGTAGAACTTGTTAGTGGCTCACAATCAGCTCTAGGCCCACCTCCTGCTGCCTCAAATTCT
  TCAGACCTGTTTGATCTTATGGGCTCGTCCCAGGCAACCATGACATCTTCCCAGAGTATGAATTTCTCTA
  TGATGAGCACTAACACTGTGGGACTTGGTTTGCCTATGTCAAGATCACAGAATACAGATATGGTCCAGAA
  ATCAGTCAGCAAAACCTTGCCCTCTACTTGGTCTGACCCCAGTGTAAACATCAGCCTAGACAACTTACTA
  CCTGGTATGCAGCCTTCCAAACCCCAGCAGCCATCACTGAATACAATGATTCAGCAACAGAATATGCAGC
  AGCCTATGAATGTGATGACTCAAAGTTTTGGAGCTGTGAACCTCAGTTCTCCATCGAACATGCTTCCTGT
  CCGGCCCCAAACTAATGCTTTGATAGGGGGACCCATGCCTATGAGCATGCCCAATGTGATGACTGGCACC
  ATGGGAATGGCCCCTCTTGGAAATACTCCGATGATGAACCAGAGCATGATGGGCATGAACATGAACATAG
  GGATGTCCGCTGCTGGGATGGGCTTGACAGGCACAATGGGAATGGGCATGCCCAACATAGCCATGACTTC
  TGGAACTGTGCAACCCAAGCAAGATGCCTTTGCAAATTTCGCCAATTTTAGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202387 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLNMWKVRELVDKATNVVMNYSEIESKVREATNDDPWGPSGQLMGEIAKATFMYEQFPELMNMLWSRMLK
  DNKKNWRRVYKSLLLLAYLIRNGSERVVTSAREHIYDLRSLENYHFVDEHGKDQGINIRQKVKELVEFAQ
  DDDRLREERKKAKKNKDKYVGVSSDSVGGFRYSERYDPEPKSKWDEEWDKNKSAFPFSDKLGELSDKIGS
  TIDDTISKFRRKDREDSPERCSDSDEEKKARRGRSPKGEFKDEEETVTTKHIHITQATETTTTRHKRTAN
  PSKTIDLGAAAHYTGDKASPDQNASTHTPQSSVKTSVPSSKSSGDLVDLFDGTSQSTGGSADLFGGFADF
  GSAAASGSFPSQVTATSGNGDFGDWSAFNQAPSGPVASSGEFFGSASQPAVELVSGSQSALGPPPAASNS
  SDLFDLMGSSQATMTSSQSMNFSMMSTNTVGLGLPMSRSQNTDMVQKSVSKTLPSTWSDPSVNISLDNLL
  PGMQPSKPQQPSLNTMIQQQNMQQPMNVMTQSFGAVNLSSPSNMLPVRPQTNALIGGPMPMSMPNVMTGT
  MGMAPLGNTPMMNQSMMGMNMNIGMSAAGMGLTGTMGMGMPNIAMTSGTVQPKQDAFANFANFSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014666
• ORF Size: 1875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014666.1, NM_014666.2, NM_014666.3, NP_055481.1
• RefSeq Size: 4007 bp
• RefSeq ORF: 1878 bp
• Locus ID: 9685
• Domains: ENTH
• MW: 68.3 kDa
• Gene Summary: This gene encodes a protein with similarity to the epsin family of endocytic adapter proteins. The encoded protein interacts with clathrin, the adapter protein AP-1 and phosphoinositides. This protein may be involved in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in this gene are associated with a susceptibility to schizophrenia and psychotic disorders. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]