CDC45L (CDC45) (NM_003504) Human Tagged ORF Clone

CAT#: RC202442L3

Lenti ORF clone of Human cell division cycle 45 homolog (S. cerevisiae) (CDC45), transcript variant 2, Myc-DDK-tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDC45
• Synonyms: CDC45L; CDC45L2; MGORS7; PORC-PI-1
• Vector: pLenti-C-Myc-DDK-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC202442).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_003504
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003504.3, NP_003495.1
• RefSeq Size: 1998
• RefSeq ORF: 1701
• Locus ID: 8318
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• MW: 65.6 kDa
• Gene Summary: The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]