Creatine kinase M type (CKM) (NM_001824) Human Tagged ORF Clone
CAT#: RC202721
CKM (Myc-DDK-tagged)-Human creatine kinase, muscle (CKM)
"NM_001824" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CKM |
Synonyms | CKMM; CPK-M; M-CK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202721 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCATTCGGTAACACCCACAACAAGTTCAAGCTGAATTACAAGCCTGAGGAGGAGTACCCCGACCTCA GCAAACATAACAACCACATGGCCAAGGTACTGACCCTTGAACTCTACAAGAAGCTGCGGGACAAGGAGAC TCCATCTGGCTTCACTGTAGACGATGTCATCCAGACAGGAGTGGACAACCCAGGTCACCCCTTCATCATG ACCGTGGGCTGCGTGGCTGGTGATGAGGAGTCCTACGAAGTTTTCAAGGAACTCTTTGACCCCATCATCT CGGATCGCCACGGGGGCTACAAACCCACTGACAAGCACAAGACTGACCTCAACCATGAAAACCTCAAGGG TGGAGACGACCTGGACCCCAACTACGTGCTCAGCAGCCGCGTCCGCACTGGCCGCAGCATCAAGGGCTAC ACGTTGCCCCCACACTGCTCCCGTGGCGAGCGCCGGGCGGTGGAGAAGCTCTCTGTGGAAGCTCTCAACA GCCTGACGGGCGAGTTCAAAGGGAAGTACTACCCTCTGAAGAGCATGACGGAGAAGGAGCAGCAGCAGCT CATCGATGACCACTTCCTGTTCGACAAGCCCGTGTCCCCGCTGCTGCTGGCCTCAGGCATGGCCCGCGAC TGGCCCGACGCCCGTGGCATCTGGCACAATGACAACAAGAGCCTCCTGGTGTGGGTGAACGAGGAGGATC ACCTCCGGGTCATCTCCATGGAGAAGGGGGGCAACATGAAGGAGGTTTTCCGCCGCTTCTGCGTAGGGCT GCAGAAGATTGAGGAGATCTTTAAGAAAGCTGGCCACCCCTTCATGTGGAACCAGCACCTGGGCTACGTG CTCACCTGCCCATCCAACCTGGGCACTGGGCTGCGTGGAGGCGTGCATGTGAAGCTGGCGCACCTGAGCA AGCACCCCAAGTTCGAGGAGATCCTCACCCGCCTGCGTCTGCAGAAGAGGGGTACAGGTGGCGTGGACAC AGCTGCCGTGGGCTCAGTATTTGACGTGTCCAACGCTGATCGGCTGGGCTCGTCCGAAGTAGAACAGGTG CAGCTGGTGGTGGATGGTGTGAAGCTCATGGTGGAAATGGAGAAGAAGTTGGAGAAAGGCCAGTCCATCG ACGACATGATCCCCGCCCAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202721 protein sequence
Red=Cloning site Green=Tags(s) MPFGNTHNKFKLNYKPEEEYPDLSKHNNHMAKVLTLELYKKLRDKETPSGFTVDDVIQTGVDNPGHPFIM TVGCVAGDEESYEVFKELFDPIISDRHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGY TLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEKEQQQLIDDHFLFDKPVSPLLLASGMARD WPDARGIWHNDNKSLLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNQHLGYV LTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQV QLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001824 |
ORF Size | 1143 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001824.1, NM_001824.2, NM_001824.3, NM_001824.4, NP_001815.2 |
RefSeq Size | 1666 bp |
RefSeq ORF | 1146 bp |
Locus ID | 1158 |
Cytogenetics | 19q13.32 |
Domains | ATP-gua_Ptrans |
Protein Families | Druggable Genome |
Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
MW | 43.1 kDa |
Gene Summary | 'The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119007 | CKM (untagged)-Human creatine kinase, muscle (CKM) |
USD 310.00 |
|
SC324597 | CKM (untagged)-Human creatine kinase, muscle (CKM) |
USD 310.00 |
|
RG202721 | CKM (GFP-tagged) - Human creatine kinase, muscle (CKM) |
USD 460.00 |
|
RC202721L1 | Lenti ORF clone of Human creatine kinase, muscle (CKM), Myc-DDK-tagged |
USD 620.00 |
|
RC202721L2 | Lenti ORF clone of Human creatine kinase, muscle (CKM), mGFP tagged |
USD 620.00 |
|
RC202721L3 | Lenti ORF clone of Human creatine kinase, muscle (CKM), Myc-DDK-tagged |
USD 620.00 |
|
RC202721L4 | Lenti ORF clone of Human creatine kinase, muscle (CKM), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review