IMPA1 (NM_005536) Human Tagged ORF Clone
CAT#: RC202784
IMPA1 (Myc-DDK-tagged)-Human inositol(myo)-1(or 4)-monophosphatase 1 (IMPA1), transcript variant 1
"NM_005536" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | IMPA1 |
Synonyms | IMP; IMPA; MRT59 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202784 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGATCCTTGGCAGGAATGCATGGATTATGCAGTAACTCTAGCAAGACAAGCTGGAGAGGTAGTTT GTGAAGCTATAAAAAATGAAATGAATGTTATGCTGAAAAGTTCTCCAGTTGATTTGGTAACTGCTACGGA CCAAAAAGTTGAAAAAATGCTTATCTCTTCCATAAAGGAAAAGTATCCATCTCACAGTTTCATTGGTGAA GAATCTGTGGCAGCTGGGGAAAAAAGTATCTTAACCGACAACCCCACATGGATCATTGACCCTATTGATG GAACAACTAACTTTGTACATAGATTTCCTTTTGTAGCTGTTTCAATTGGCTTTGCTGTAAATAAAAAGAT AGAATTTGGAGTTGTGTACAGTTGTGTGGAAGGCAAGATGTACACTGCCAGAAAAGGAAAAGGTGCCTTT TGTAATGGTCAAAAACTACAAGTCTCACAACAAGAAGATATTACCAAATCTCTCTTGGTGACTGAGTTGG GCTCTTCCAGAACACCAGAGACTGTGAGAATGGTTCTTTCTAATATGGAAAAGCTTTTTTGCATTCCTGT TCATGGGATCCGGAGTGTTGGAACAGCAGCTGTTAATATGTGCCTTGTGGCAACTGGCGGAGCAGATGCA TATTATGAAATGGGAATTCACTGCTGGGATGTTGCAGGAGCTGGCATTATTGTTACTGAAGCTGGTGGCG TGCTAATGGATGTTACAGGTGGACCATTTGATTTGATGTCACGAAGAGTAATTGCTGCAAATAATAGAAT ATTAGCAGAAAGGATAGCTAAAGAAATTCAGGTTATACCTTTGCAACGAGACGACGAAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202784 protein sequence
Red=Cloning site Green=Tags(s) MADPWQECMDYAVTLARQAGEVVCEAIKNEMNVMLKSSPVDLVTATDQKVEKMLISSIKEKYPSHSFIGE ESVAAGEKSILTDNPTWIIDPIDGTTNFVHRFPFVAVSIGFAVNKKIEFGVVYSCVEGKMYTARKGKGAF CNGQKLQVSQQEDITKSLLVTELGSSRTPETVRMVLSNMEKLFCIPVHGIRSVGTAAVNMCLVATGGADA YYEMGIHCWDVAGAGIIVTEAGGVLMDVTGGPFDLMSRRVIAANNRILAERIAKEIQVIPLQRDDED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005536 |
ORF Size | 831 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005536.1, NM_005536.2, NM_005536.3, NP_005527.1 |
RefSeq Size | 3396 bp |
RefSeq ORF | 834 bp |
Locus ID | 3612 |
Cytogenetics | 8q21.13 |
Domains | inositol_P |
Protein Families | Druggable Genome |
Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
MW | 30.2 kDa |
Gene Summary | 'This gene encodes an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13. [provided by RefSeq, Dec 2014]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116674 | IMPA1 (untagged)-Human inositol(myo)-1(or 4)-monophosphatase 1 (IMPA1), transcript variant 1 |
USD 310.00 |
|
RG202784 | IMPA1 (GFP-tagged) - Human inositol(myo)-1(or 4)-monophosphatase 1 (IMPA1), transcript variant 1 |
USD 460.00 |
|
RC202784L3 | Lenti ORF clone of Human inositol(myo)-1(or 4)-monophosphatase 1 (IMPA1), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC202784L4 | Lenti ORF clone of Human inositol(myo)-1(or 4)-monophosphatase 1 (IMPA1), transcript variant 1, mGFP tagged |
USD 620.00 |
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