AP1M2 (NM_005498) Human Tagged ORF Clone

CAT#: RC202818

AP1M2 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, mu 2 subunit (AP1M2)

Product Images

Western blot validation of overexpression lysate (Cat# LY417266) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202818 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AP1M2
• Synonyms: AP1-mu2; HSMU1B; MU-1B; MU1B; mu2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202818 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCTCGGCTGTCTTCATTCTGGACGTTAAGGGCAAGCCATTGATCAGCCGCAACTACAAGGGCG
  ATGTGGCCATGAGCAAGATTGAGCACTTCATGCCTTTGCTGGTACAGCGGGAGGAGGAAGGCGCCCTGGC
  CCCGCTGCTGAGCCACGGCCAGGTCCACTTCCTATGGATCAAACACAGCAACCTCTACTTGGTGGCCACC
  ACATCGAAGAATGCCAATGCCTCCCTGGTGTACTCCTTCCTGTATAAGACAATAGAGGTATTCTGCGAAT
  ACTTCAAGGAGCTGGAGGAGGAGAGCATCCGGGACAACTTTGTCATCGTCTACGAGTTGCTGGACGAGCT
  CATGGACTTTGGCTTCCCGCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGCAGAGCAAC
  AAGCTGGAGACGGGCAAGTCACGGGTGCCACCCACTGTCACCAACGCTGTGTCCTGGCGCTCCGAGGGTA
  TCAAGTATAAGAAGAACGAGGTCTTCATTGATGTCATAGAGTCTGTCAACCTGCTGGTCAATGCCAACGG
  CAGCGTCCTTCTGAGCGAAATCGTCGGTACCATCAAGCTCAAGGTGTTTCTGTCGGGAATGCCAGAGCTG
  CGGCTGGGCCTCAATGACCGCGTGCTCTTCGAGCTCACTGGCCTTTCAGGCAGCAAGAACAAATCAGTAG
  AGCTGGAGGATGTAAAATTCCACCAGTGCGTGCGGCTCTCTCGCTTTGACAACGACCGCACCATCTCCTT
  CATCCCGCCTGATGGTGACTTTGAGCTCATGTCATACCGCCTCAGCACCCAGGTCAAGCCACTGATCTGG
  ATTGAGTCTGTCATTGAGAAGTTCTCCCACAGCCGCGTGGAGATCATGGTCAAGGCCAAGGGGCAGTTTA
  AGAAACAGTCAGTGGCCAACGGTGTGGAGATATCTGTGCCTGTACCCAGCGATGCCGACTCCCCCAGATT
  CAAGACCAGTGTGGGCAGCGCCAAGTATGTGCCGGAGAGAAACGTCGTGATTTGGAGTATTAAGTCTTTC
  CCGGGGGGCAAGGAGTACTTGATGCGAGCCCACTTTGGCCTCCCCCGTGTGGAAAAGGAAGAGGTGGAGG
  GCCGGCCCCCCATCGGGGTCAAGTTTGAGATCCCCTACTTCACCGTCTCTGGGATCCAGGTCCGATACAT
  GAAGATCATTGAGAAAAGTGGTTACCAGGCCCTGCCCTGGGTTCGCTACATCACCCAGAGTGGCGATTAC
  CAACTTCGTACCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202818 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSASAVFILDVKGKPLISRNYKGDVAMSKIEHFMPLLVQREEEGALAPLLSHGQVHFLWIKHSNLYLVAT
  TSKNANASLVYSFLYKTIEVFCEYFKELEEESIRDNFVIVYELLDELMDFGFPQTTDSKILQEYITQQSN
  KLETGKSRVPPTVTNAVSWRSEGIKYKKNEVFIDVIESVNLLVNANGSVLLSEIVGTIKLKVFLSGMPEL
  RLGLNDRVLFELTGLSGSKNKSVELEDVKFHQCVRLSRFDNDRTISFIPPDGDFELMSYRLSTQVKPLIW
  IESVIEKFSHSRVEIMVKAKGQFKKQSVANGVEISVPVPSDADSPRFKTSVGSAKYVPERNVVIWSIKSF
  PGGKEYLMRAHFGLPRVEKEEVEGRPPIGVKFEIPYFTVSGIQVRYMKIIEKSGYQALPWVRYITQSGDY
  QLRTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005498
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005498.1, NM_005498.2, NM_005498.3, NM_005498.4, NP_005489.2
• RefSeq Size: 1756 bp
• RefSeq ORF: 1272 bp
• Locus ID: 10053
• Domains: Adap_comp_sub
• Protein Pathways: Lysosome
• MW: 48.3 kDa
• Gene Summary: This gene encodes a subunit of the heterotetrameric adaptor-related protein comlex 1 (AP-1), which belongs to the adaptor complexes medium subunits family. This protein is capable of interacting with tyrosine-based sorting signals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]