MRPS30 (NM_016640) Human Tagged ORF Clone

CAT#: RC202830

MRPS30 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S30 (MRPS30), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY413812) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202830 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MRPS30 protein (Cat# TP302830). The protein was produced from HEK293T cells transfected with MRPS30 cDNA clone (Cat# RC202830) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MRPS30
• Synonyms: MRP-S30; PAP; PDCD9; S30mt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202830 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCCAGGTGTTGGAGGCCTTTGCTACGCGGTCCGAGGCTTTCATTGCACACCGCGGCTAATG
  CCGCCGCCACGGCTACAGAAACGACCTGCCAAGACGTCGCGGCGACCCCCGTCGCGCGGTACCCGCCGAT
  TGTGGCCTCCATGACAGCCGACAGCAAAGCTGCACGGCTGCGGCGGATCGAGCGCTGGCAGGCGACGGTG
  CACGCTGCGGAGTCGGTAGACGAGAAGCTGCGAATCCTCACCAAGATGCAGTTTATGAAGTACATGGTTT
  ACCCGCAGACCTTCGCGCTGAATGCCGACCGCTGGTACCAGTACTTCACCAAGACCGTGTTCCTGTCGGG
  TCTGCCGCCGCCCCCAGCGGAGCCCGAGCCCGAGCCCGAACCCGAACCTGAACCTGCGCTGGACCTCGCG
  GCGCTGCGTGCGGTCGCCTGCGACTGCCTGCTGCAGGAGCACTTCTACCTGCGGCGCAGGCGGCGCGTGC
  ACCGTTACGAGGAGAGCGAGGTCATATCTTTGCCCTTCCTGGATCAGCTGGTGTCAACCCTCGTGGGCCT
  CCTCAGCCCACACAACCCGGCCCTGGCCGCTGCCGCCCTCGATTATAGATGCCCAGTTCATTTTTACTGG
  GTGCGTGGTGAAGAAATTATTCCTCGTGGTCATCGAAGAGGTCGAATTGATGACTTGCGATACCAGATAG
  ATGATAAACCAAACAACCAGATTCGAATATCCAAGCAACTCGCAGAGTTTGTGCCATTGGATTATTCTGT
  TCCTATAGAAATCCCCACTATAAAATGTAAACCAGACAAACTTCCATTATTCAAACGGCAGTATGAAAAC
  CACATATTTGTTGGCTCAAAAACTGCAGATCCTTGCTGTTACGGTCACACCCAGTTTCATCTGTTACCTG
  ACAAATTAAGAAGGGAAAGGCTTTTGAGACAAAACTGTGCTGATCAGATAGAAGTTGTTTTTAGAGCTAA
  TGCTATTGCAAGCCTTTTTGCTTGGACTGGAGCACAAGCTATGTATCAAGGATTCTGGAGTGAAGCAGAT
  GTTACTCGACCTTTTGTCTCCCAGGCTGTGATCACAGATGGAAAATACTTTTCCTTTTTCTGCTACCAGC
  TAAATACTTTGGCACTGACTACACAAGCTGATCAAAATAACCCTCGTAAAAATATATGTTGGGGTACACA
  AAGTAAGCCTCTTTATGAAACAATTGAGGATAATGATGTGAAAGGTTTTAATGATGATGTTCTACTTCAG
  ATAGTTCACTTTCTACTGAATAGACCAAAAGAAGAAAAATCACAGCTGTTGGAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202830 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAARCWRPLLRGPRLSLHTAANAAATATETTCQDVAATPVARYPPIVASMTADSKAARLRRIERWQATV
  HAAESVDEKLRILTKMQFMKYMVYPQTFALNADRWYQYFTKTVFLSGLPPPPAEPEPEPEPEPEPALDLA
  ALRAVACDCLLQEHFYLRRRRRVHRYEESEVISLPFLDQLVSTLVGLLSPHNPALAAAALDYRCPVHFYW
  VRGEEIIPRGHRRGRIDDLRYQIDDKPNNQIRISKQLAEFVPLDYSVPIEIPTIKCKPDKLPLFKRQYEN
  HIFVGSKTADPCCYGHTQFHLLPDKLRRERLLRQNCADQIEVVFRANAIASLFAWTGAQAMYQGFWSEAD
  VTRPFVSQAVITDGKYFSFFCYQLNTLALTTQADQNNPRKNICWGTQSKPLYETIEDNDVKGFNDDVLLQ
  IVHFLLNRPKEEKSQLLEN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016640
• ORF Size: 1317 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016640.1, NM_016640.2, NM_016640.3, NP_057724.2
• RefSeq Size: 1686 bp
• RefSeq ORF: 1320 bp
• Locus ID: 10884
• MW: 50.4 kDa
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available. [provided by RefSeq, Jul 2008]