VAX2 (NM_012476) Human Tagged ORF Clone

CAT#: RC202834

VAX2 (Myc-DDK-tagged)-Human ventral anterior homeobox 2 (VAX2)

Product Images

Western blot validation of overexpression lysate (Cat# LY415736) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202834 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VAX2
• Synonyms: DRES93
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202834 representing NM_012476
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGATGGGGGCGCCGAGCGCGACCGGGGCCCCGCGCGCCGGGCGGAGTCTGGTGGCGGCGGTGGGC
  GCTGCGGAGACCGCAGCGGAGCGGGGGACTTGCGAGCTGATGGCGGTGGCCACAGCCCAACGGAGGTGGC
  CGGGACCTCAGCCTCCAGTCCCGCAGGCTCCAGGGAGAGTGGAGCCGACAGCGACGGGCAGCCCGGGCCC
  GGCGAGGCAGACCACTGCCGCCGCATACTGGTGCGAGATGCCAAAGGGACAATTCGGGAAATTGTCCTGC
  CTAAGGGCCTGGACCTGGACCGGCCCAAGCGGACACGTACATCCTTCACTGCCGAGCAGCTGTACCGCCT
  GGAGATGGAGTTCCAGCGCTGCCAGTATGTGGTGGGCCGCGAGCGCACTGAGCTGGCCCGCCAGCTGAAC
  CTCTCCGAGACCCAGGTGAAGGTCTGGTTCCAGAACCGCCGCACCAAGCAGAAGAAAGACCAGAGCAGAG
  ACCTGGAGAAGCGGGCGTCCTCCTCAGCCTCCGAGGCCTTTGCCACCTCCAACATTCTGCGGCTGCTGGA
  GCAGGGCCGGCTGCTCTCTGTGCCCAGGGCCCCTAGCCTCCTGGCGCTGACCCCTAGCCTGCCAGGCCTA
  CCTGCCAGCCACAGGGGCACCTCCTTAGGTGACCCCAGGAACTCCTCCCCACGCCTCAACCCGCTGTCCT
  CGGCCTCAGCGTCCCCCCCACTGCCGCCCCCTCTGCCAGCTGTCTGCTTTTCCTCGGCCCCGCTCCTGGA
  TCTGCCTGCCGGCTACGAACTGGGTTCCTCGGCCTTCGAGCCATACAGCTGGCTAGAACGGAAAGTGGGC
  AGCGCCAGCAGCTGCAAGAAAGCTAACACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202834 representing NM_012476
  Red=Cloning site Green=Tags(s)
  
  MGDGGAERDRGPARRAESGGGGGRCGDRSGAGDLRADGGGHSPTEVAGTSASSPAGSRESGADSDGQPGP
  GEADHCRRILVRDAKGTIREIVLPKGLDLDRPKRTRTSFTAEQLYRLEMEFQRCQYVVGRERTELARQLN
  LSETQVKVWFQNRRTKQKKDQSRDLEKRASSSASEAFATSNILRLLEQGRLLSVPRAPSLLALTPSLPGL
  PASHRGTSLGDPRNSSPRLNPLSSASASPPLPPPLPAVCFSSAPLLDLPAGYELGSSAFEPYSWLERKVG
  SASSCKKANT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012476
• ORF Size: 870 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012476.1, NM_012476.2, NP_036608.1
• RefSeq Size: 1164
• RefSeq ORF: 873
• Locus ID: 25806
• Protein Families: Transcription Factors
• MW: 30.7 kDa
• Gene Summary: This gene encodes a homeobox protein and is almost exclusively expressed in the ventral portion of the retina during development. In mouse studies, this gene was found to be required for the correct formation of the optic fissure and other aspects of retinal development. [provided by RefSeq, Sep 2008]