FAIM3 (FCMR) (NM_005449) Human Tagged ORF Clone

CAT#: RC202905

FAIM3 (Myc-DDK-tagged)-Human Fas apoptotic inhibitory molecule 3 (FAIM3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY428118) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227937 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FCMR
• Synonyms: FAIM3; TOSO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202905 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTCTGGCTTTGGCCACTTTACTTCCTGCCAGTATCGGGGGCCCTGAGGATCCTCCCAGAAGTAA
  AGGTAGAGGGGGAGCTGGGCGGATCAGTTACCATCAAGTGCCCACTTCCTGAAATGCATGTGAGGATATA
  TCTGTGCCGGGAGATGGCTGGATCTGGAACATGTGGTACCGTGGTATCCACCACCAACTTCATCAAGGCA
  GAATACAAGGGCCGAGTTACTCTGAAGCAATACCCACGCAAGAATCTGTTCCTAGTGGAGGTAACACAGC
  TGACAGAAAGTGACAGCGGAGTCTATGCCTGCGGAGCGGGCATGAACACAGACCGGGGAAAGACCCAGAA
  AGTCACCCTGAATGTCCACAGTGAATACGAGCCATCATGGGAAGAGCAGCCAATGCCTGAGACTCCAAAA
  TGGTTTCATCTGCCCTATTTGTTCCAGATGCCTGCATATGCCAGTTCTTCCAAATTCGTAACCAGAGTTA
  CCACACCAGCTCAAAGGGGCAAGGTCCCTCCAGTTCACCACTCCTCCCCCACCACCCAAATCACCCACCG
  CCCTCGAGTGTCCAGAGCATCTTCAGTAGCAGGTGACAAGCCCCGAACCTTCCTGCCATCCACTACAGCC
  TCAAAAATCTCAGCTCTGGAGGGGCTGCTCAAGCCCCAGACGCCCAGCTACAACCACCACACCAGGCTGC
  ACAGGCAGAGAGCACTGGACTATGGCTCACAGTCTGGGAGGGAAGGCCAAGGATTTCACATCCTGATCCC
  GACCATCCTGGGCCTTTTCCTGCTGGCACTTCTGGGGCTGGTGGTGAAAAGGGCCGTTGAAAGGAGGAAA
  GCCCTCTCCAGGCGGGCCCGCCGACTGGCCGTGAGGATGCGCGCCCTGGAGAGCTCCCAGAGGCCCCGCG
  GGTCGCCGCGACCGCGCTCCCAAAACAACATCTACAGCGCCTGCCCGCGGCGCGCTCGTGGAGCGGACGC
  TGCAGGCACAGGGGAGGCCCCCGTTCCCGGCCCCGGAGCGCCGTTGCCCCCCGCCCCGCTGCAGGTGTCT
  GAATCTCCCTGGCTCCATGCCCCATCTCTGAAGACCAGCTGTGAATACGTGAGCCTCTACCACCAGCCTG
  CCGCCATGATGGAGGACAGTGATTCAGATGACTACATCAATGTTCCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202905 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDFWLWPLYFLPVSGALRILPEVKVEGELGGSVTIKCPLPEMHVRIYLCREMAGSGTCGTVVSTTNFIKA
  EYKGRVTLKQYPRKNLFLVEVTQLTESDSGVYACGAGMNTDRGKTQKVTLNVHSEYEPSWEEQPMPETPK
  WFHLPYLFQMPAYASSSKFVTRVTTPAQRGKVPPVHHSSPTTQITHRPRVSRASSVAGDKPRTFLPSTTA
  SKISALEGLLKPQTPSYNHHTRLHRQRALDYGSQSGREGQGFHILIPTILGLFLLALLGLVVKRAVERRK
  ALSRRARRLAVRMRALESSQRPRGSPRPRSQNNIYSACPRRARGADAAGTGEAPVPGPGAPLPPAPLQVS
  ESPWLHAPSLKTSCEYVSLYHQPAAMMEDSDSDDYINVPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005449
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005449.1, NM_005449.2, NM_005449.3, NM_005449.4, NP_005440.1
• RefSeq Size: 3109 bp
• RefSeq ORF: 1173 bp
• Locus ID: 9214
• Domains: ig, IG
• Protein Families: Druggable Genome, Transmembrane
• MW: 43.1 kDa
• Gene Summary: Fc receptors specifically bind to the Fc region of immunoglobulins (Igs) to mediate the unique functions of each Ig class. FAIM3 encodes an Fc receptor for IgM (see MIM 147020) (Kubagawa et al., 2009 [PubMed 19858324]; Shima et al., 2010 [PubMed 20042454]). [supplied by OMIM, Jul 2010]