PLTP (NM_182676) Human Tagged ORF Clone

CAT#: RC202925

PLTP (Myc-DDK-tagged)-Human phospholipid transfer protein (PLTP), transcript variant 2


  "NM_182676" in other vectors (4)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PLTP
Synonyms BPIFE; HDLCQ9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202925 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTCTTCGGGGCCCTCTTCCTAGCGCTGCTGGCAGGCGCACATGCAGAGTTCCCAGGCTGCAAGA
TCCGCGTCACCTCCAAGGCGCTGGAGCTGGTGAAGCAGGAGGGGCTGCGCTTTCTGGAGCAAGAGCTGGA
GACTATCACCATTCCGGACCTGCGGGGCAAAGAAGGCCACTTCTACTACAACATCTCTGAGGTGAAGGTC
ACAGAGCTGCAACTGACATCTTCCGAGCTCGATTTCCAGCCACAGCAGGAGCTGATGCTTCAAATCACCA
ATGCCTCCTTGGGGCTGCGCTTCCGGAGACAGCTGCTCTACTGGTTCTTGAAGGTGTATGATTTTCTCTC
CACGTTCATCACCTCAGGGATGCGCTTCCTCCTCAACCAGCAGATCTGCCCTGTCCTCTACCACGCAGGG
ACGGTCCTGCTCAACTCCCTCCTGGACACCGTGCCTGTGCGCAGTTCTGTGGACGAGCTTGTTGGCATTG
ACTATTCCCTCATGAAGGATCCTGTGGCTTCCACCAGCAACCTGGACATGGACTTCCGGGGGGCCTTCTT
CCCCCTGACTGAGAGGAACTGGAGCCTCCCCAACCGGGCAGTGGAGCCCCAGCTGCAGGAGGAAGAGCGG
ATGGTGTATGTGGCCTTCTCTGAGTTCTTCTTCGACTCTGCCATGGAGAGCTACTTCCGGGCGGGGGCCC
TGCAGCTGTTGCTGGTGGGGGACAAGGTGCCCCACGACCTGGACATGCTGCTGAGGGCCACCTACTTTGG
GAGCATTGTCCTGCTGAGCCCAGCAGTGATTGACTCCCCATTGAAGCTGGAGCTGCGGGTCCTGGCCCCA
CCGCGCTGCACCATCAAGCCCTCTGGCACCACCATCTCTGTCACTGCTAGCGTCACCATTGCCCTGGTCC
CACCAGACCAGCCTGAGGTCCAGCTGTCCAGCATGACTATGGACGCCCGTCTCAGCGCCAAGATGGCTCT
CCGGGGGAAGGCCCTGCGCACGCAGCTGGACCTGCGCAGGTTCCGAATCTATTCCAACCATTCTGCACTG
GAGTCGCTGGCTCTGATCCCATTACAGGCCCCTCTGAAGACCATGCTGCAGATTGGGGTGATGCCCATGC
TCAATGAGCGGACCTGGCGTGGGGTGCAGATCCCACTACCTGAGGGCATCAACTTTGTGCATGAGGTGGT
GACGAACCATGCGGGATTCCTCACCATCGGGGCTGATCTCCACTTTGCCAAAGGGCTGCGAGAGGTGATT
GAGAAGAACCGGCCTGCTGATGTCAGGGCGTCCACTGCCCCCACACCGTCCACAGCAGCTGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202925 protein sequence
Red=Cloning site Green=Tags(s)

MALFGALFLALLAGAHAEFPGCKIRVTSKALELVKQEGLRFLEQELETITIPDLRGKEGHFYYNISEVKV
TELQLTSSELDFQPQQELMLQITNASLGLRFRRQLLYWFLKVYDFLSTFITSGMRFLLNQQICPVLYHAG
TVLLNSLLDTVPVRSSVDELVGIDYSLMKDPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEER
MVYVAFSEFFFDSAMESYFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAP
PRCTIKPSGTTISVTASVTIALVPPDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSNHSAL
ESLALIPLQAPLKTMLQIGVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTIGADLHFAKGLREVI
EKNRPADVRASTAPTPSTAAV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_182676
ORF Size 1323 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_182676.1, NM_182676.2, NP_872617.1
RefSeq Size 1944 bp
RefSeq ORF 1326 bp
Locus ID 5360
Cytogenetics 20q13.12
Protein Families Druggable Genome, Secreted Protein
Protein Pathways PPAR signaling pathway
MW 49.3 kDa
Gene Summary 'The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'

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