RTBDN (NM_031429) Human Tagged ORF Clone

CAT#: RC202964

RTBDN (Myc-DDK-tagged)-Human retbindin (RTBDN), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY403115) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202964 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RTBDN
• Synonyms: FLJ36353
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202964 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAAGCCCTAGAAACGCAGCTGAAGACGAGCAGAGGACGCTTCTCGGCTACAGAATCCCTCCCCA
  CCTTGGAGCTCTTATCTCAGGTGGACATGGACTGCAGGGTCCACATGCGACCCATCGGCCTGACGTGGGT
  GCTGCAACTGACCTTGGCATGGATCCTGCTAGAAGCCTGTGGAGGGAGCCGCCCACTCCAAGCCAGGTCC
  CAGCAACACCATGGGCTGGCAGCTGATCTGGGCAAAGGCAAGCTGCACCTGGCAGGACCTTGTTGTCCCT
  CAGAGATGGACACAACAGAGACATCGGGCCCTGGAAACCATCCAGAACGCTGTGGAGTGCCGAGCCCTGA
  ATGCGAATCCTTCCTGGAACACCTCCAACGTGCCCTTCGCAGTCGCTTCCGCCTGCGGCTATTGGGGGTA
  CGCCAGGCACAGCCGCTCTGCGAGGAGCTCTGCCAGGCCTGGTTCGCCAACTGCGAAGATGATATCACCT
  GCGGCCCGACTTGGCTCCCACTCTCAGAAAAAAGGGGCTGTGAGCCCAGCTGCCTTACCTATGGACAGAC
  CTTCGCAGACGGGACGGACCTTTGTCGCTCGGCTCTGGGCCACGCCCTACCGGTGGCTGCTCCTGGAGCC
  CGTCACTGCTTCAACATCTCCATCTCCGCGGTACCTCGTCCCAGACCAGGACGACGGGGCCGGGAAGCTC
  CCTCCCGGCGTTCCCGCAGCCCTCGCACCTCCATCCTGGACGCTGCGGGCAGCGGGAGTGGCAGTGGAAG
  CGGCAGCGGCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202964 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDEALETQLKTSRGRFSATESLPTLELLSQVDMDCRVHMRPIGLTWVLQLTLAWILLEACGGSRPLQARS
  QQHHGLAADLGKGKLHLAGPCCPSEMDTTETSGPGNHPERCGVPSPECESFLEHLQRALRSRFRLRLLGV
  RQAQPLCEELCQAWFANCEDDITCGPTWLPLSEKRGCEPSCLTYGQTFADGTDLCRSALGHALPVAAPGA
  RHCFNISISAVPRPRPGRRGREAPSRRSRSPRTSILDAAGSGSGSGSGSGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031429
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031429.1, NM_031429.2, NP_113617.1
• RefSeq Size: 1362 bp
• RefSeq ORF: 786 bp
• Locus ID: 83546
• Domains: Folate_rec
• Protein Families: Druggable Genome, Secreted Protein
• MW: 28.2 kDa
• Gene Summary: This gene was first identified in a study of human eye tissues. The protein encoded by this gene is preferentially expressed in the retina and may play a role in binding retinoids and other carotenoids as it shares homology with riboflavin binding proteins. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jul 2012]