AGMAT (NM_024758) Human Tagged ORF Clone

CAT#: RC202990

AGMAT (Myc-DDK-tagged)-Human agmatine ureohydrolase (agmatinase) (AGMAT)

Product Images

Western blot validation of overexpression lysate (Cat# LY411072) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202990 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AGMAT protein (Cat# TP302990). The protein was produced from HEK293T cells transfected with AGMAT cDNA clone (Cat# RC202990) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AGMAT
• Synonyms: FLJ23384
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202990 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGCTGCTGGCGTCCGGGTGCGCCCGGGGCCCGGGGCCCGGCGTGGGCGCGCGTCCTGCCGCAG
  GGCTCTTTCATCCGGGGCGCCGCCAGAGCCGCCAGGCTTCCGATGCGCCCCGGAACCAGCCCCCCAGCCC
  CGAGTTCGTGGCCCGGCCGGTGGGCGTCTGCTCCATGATGCGCCTGCCGGTGCAGACCTCCCCCGAGGGG
  CTGGACGCTGCCTTCATCGGGGTGCCCCTGGATACTGGGACCTCCAACCGGCCTGGGGCGAGATTCGGAC
  CTCGCCGCATCCGGGAAGAATCAGTGATGCTTCGGACAGTCAATCCTAGCACGGGGGCCCTCCCCTTCCA
  GTCCCTCATGGTTGCAGACCTAGGCGATGTGAATGTCAATCTTTACAACCTTCAGGACAGCTGCCGGCGA
  ATTCAAGAGGCCTATGAGAAAATTGTAGCAGCTGGCTGTATTCCTCTGACCTTGGGTGGAGATCACACAA
  TCACATATCCCATATTGCAAGCGATGGCAAAAAAGCATGGCCCAGTGGGGCTGCTGCACGTGGATGCGCA
  CACGGACACGACCGACAAGGCCCTAGGAGAGAAGCTCTACCACGGGGCGCCCTTCCGCCGGTGTGTGGAT
  GAGGGTCTCCTGGACTGTAAGCGTGTGGTGCAGATTGGCATCCGGGGCTCTTCCACGACCTTGGATCCCT
  ACAGATACAACCGGAGCCAGGGCTTCCGGGTAGTCCTGGCTGAAGACTGCTGGATGAAGTCGCTGGTTCC
  TCTGATGGGGGAAGTCAGGCAGCAGATGGGAGGCAAACCCATTTATATCAGCTTTGATATTGACGCTCTG
  GATCCTGCCTATGCGCCAGGGACAGGGACACCTGAAATTGCTGGTCTCACTCCTAGTCAGGCTCTGGAGA
  TCATCAGGGGTTGTCAAGGCCTGAACGTGATGGGCTGTGATCTTGTCGAAGTTTCACCACCGTATGATCT
  TTCTGGGAACACAGCCCTGCTGGCGGCTAACCTGCTGTTTGAGATGCTATGTGCTCTCCCCAAAGTGACA
  ACCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202990 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLRLLASGCARGPGPGVGARPAAGLFHPGRRQSRQASDAPRNQPPSPEFVARPVGVCSMMRLPVQTSPEG
  LDAAFIGVPLDTGTSNRPGARFGPRRIREESVMLRTVNPSTGALPFQSLMVADLGDVNVNLYNLQDSCRR
  IQEAYEKIVAAGCIPLTLGGDHTITYPILQAMAKKHGPVGLLHVDAHTDTTDKALGEKLYHGAPFRRCVD
  EGLLDCKRVVQIGIRGSSTTLDPYRYNRSQGFRVVLAEDCWMKSLVPLMGEVRQQMGGKPIYISFDIDAL
  DPAYAPGTGTPEIAGLTPSQALEIIRGCQGLNVMGCDLVEVSPPYDLSGNTALLAANLLFEMLCALPKVT
  TV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024758
• ORF Size: 1056 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024758.1, NM_024758.2, NM_024758.3, NM_024758.4, NP_079034.3
• RefSeq Size: 3154 bp
• RefSeq ORF: 1059 bp
• Locus ID: 79814
• Domains: arginase
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• MW: 37.8 kDa