CORO1B (NM_020441) Human Tagged ORF Clone
CAT#: RC203023
CORO1B (Myc-DDK-tagged)-Human coronin, actin binding protein, 1B (CORO1B), transcript variant 1
"NM_020441" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CORO1B |
Synonyms | CORONIN-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203023 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTCCGCAAAGTGGTCCGGCAGAGCAAATTCCGGCATGTGTTCGGGCAGCCGGTCAAGAACGACC AGTGCTATGAGGACATTCGCGTGTCCCGTGTTACCTGGGACAGCACCTTCTGCGCCGTCAACCCCAAGTT CCTGGCGGTGATTGTGGAGGCCAGTGGAGGGGGTGCCTTTCTGGTGCTCCCCCTAAGCAAGACGGGCCGC ATTGACAAGGCCTACCCGACGGTGTGTGGGCACACGGGACCTGTCCTGGACATCGACTGGTGTCCTCACA ACGACGAAGTCATAGCCAGCGGCTCGGAGGACTGCACGGTCATGGTGTGGCAGATCCCAGAGAACGGGCT GACCTCCCCGCTGACAGAGCCGGTGGTGGTACTGGAGGGGCACACCAAGCGAGTGGGCATCATCGCCTGG CACCCCACGGCCCGAAACGTGCTGCTCAGTGCAGGCTGCGACAACGTGGTACTCATCTGGAATGTGGGCA CAGCGGAGGAGCTGTACCGCCTGGACAGCCTGCACCCTGACCTCATCTACAATGTCAGCTGGAACCACAA TGGCAGCCTGTTTTGCTCAGCATGCAAGGACAAGAGCGTGCGCATCATCGACCCCCGTCGGGGCACCCTG GTGGCAGAGCGGGAGAAGGCTCATGAGGGGGCCCGGCCCATGCGGGCCATCTTCCTGGCAGATGGCAAGG TGTTCACCACAGGCTTCAGCCGAATGAGCGAGCGGCAGCTGGCGCTCTGGGACCCAGAAAACCTCGAGGA ACCCATGGCCCTGCAGGAACTGGACTCGAGCAACGGGGCCCTGCTGCCCTTCTACGACCCCGACACCAGT GTGGTCTACGTCTGCGGCAAGGGTGACTCCAGCATCCGGTACTTTGAGATCACAGAGGAGCCTCCCTACA TCCACTTCCTGAACACGTTCACCAGCAAGGAGCCGCAGCGGGGTATGGGCAGCATGCCCAAGCGGGGCCT GGAGGTCAGCAAGTGCGAGATCGCCCGGTTCTACAAACTGCATGAGCGCAAGTGTGAGCCCATCGTCATG ACTGTGCCAAGAAAGTCGGACCTCTTCCAGGATGATCTGTACCCCGACACAGCCGGGCCCGAGGCAGCCC TGGAGGCTGAGGAGTGGGTGAGCGGGCGGGATGCCGACCCGATCCTCATCTCACTGCGGGAGGCCTACGT GCCCAGCAAGCAGCGGGACCTGAAGATCAGCCGGCGCAACGTGTTGTCTGACAGCCGGCCCGCCATGGCC CCGGGCTCCTCCCACCTAGGGGCCCCCGCCTCCACCACCACTGCTGCTGATGCCACCCCCAGCGGCAGCC TGGCCAGAGCCGGGGAGGCTGGGAAGCTGGAGGAGGTGATGCAGGAGCTGCGGGCCCTGAGGGCGCTGGT CAAGGAGCAGGGCGACCGCATCTGCCGCCTGGAGGAGCAGCTGGGCCGCATGGAGAACGGGGATGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203023 protein sequence
Red=Cloning site Green=Tags(s) MSFRKVVRQSKFRHVFGQPVKNDQCYEDIRVSRVTWDSTFCAVNPKFLAVIVEASGGGAFLVLPLSKTGR IDKAYPTVCGHTGPVLDIDWCPHNDEVIASGSEDCTVMVWQIPENGLTSPLTEPVVVLEGHTKRVGIIAW HPTARNVLLSAGCDNVVLIWNVGTAEELYRLDSLHPDLIYNVSWNHNGSLFCSACKDKSVRIIDPRRGTL VAEREKAHEGARPMRAIFLADGKVFTTGFSRMSERQLALWDPENLEEPMALQELDSSNGALLPFYDPDTS VVYVCGKGDSSIRYFEITEEPPYIHFLNTFTSKEPQRGMGSMPKRGLEVSKCEIARFYKLHERKCEPIVM TVPRKSDLFQDDLYPDTAGPEAALEAEEWVSGRDADPILISLREAYVPSKQRDLKISRRNVLSDSRPAMA PGSSHLGAPASTTTAADATPSGSLARAGEAGKLEEVMQELRALRALVKEQGDRICRLEEQLGRMENGDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020441 |
ORF Size | 1467 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020441.1, NM_020441.2, NP_065174.1 |
RefSeq Size | 1927 bp |
RefSeq ORF | 1470 bp |
Locus ID | 57175 |
Domains | WD40 |
MW | 54.2 kDa |
Gene Summary | Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility (Cai et al., 2005 [PubMed 16027158]). [supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC320143 | CORO1B (untagged)-Human coronin, actin binding protein, 1B (CORO1B), transcript variant 1 |
USD 310.00 |
|
RG203023 | CORO1B (GFP-tagged) - Human coronin, actin binding protein, 1B (CORO1B), transcript variant 1 |
USD 460.00 |
|
RC203023L1 | Lenti ORF clone of Human coronin, actin binding protein, 1B (CORO1B), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC203023L2 | Lenti ORF clone of Human coronin, actin binding protein, 1B (CORO1B), transcript variant 1, mGFP tagged |
USD 768.00 |
|
RC203023L3 | Lenti ORF clone of Human coronin, actin binding protein, 1B (CORO1B), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC203023L4 | Lenti ORF clone of Human coronin, actin binding protein, 1B (CORO1B), transcript variant 1, mGFP tagged |
USD 620.00 |
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