MRPL48 (NM_016055) Human Tagged ORF Clone
CAT#: RC203039
MRPL48 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L48 (MRPL48), nuclear gene encoding mitochondrial protein
"NM_016055" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MRPL48 |
Synonyms | CGI-118; HSPC290; L48MT; MRP-L48 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203039 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGAACCTTGGGAAAGGTGCTGTGCCTGAGGAACAATACCATTTTTAAGCAAGCCTTTTCTCTCT TAAGGTTTAGAACTTCAGGAGAGAAGCCCATCTATTCTGTAGGTGGAATTCTACTAAGTATCAGTCGGCC CTACAAGACAAAGCCCACCCACGGCATTGGAAAGTACAAGCACTTAATTAAAGCAGAAGAGCCCAAGAAG AAGAAGGGAAAAGTGGAAGTGAGAGCCATTAATTTGGGGACAGATTATGAATATGGGGTTTTAAATATTC ATCTGACTGCATATGATATGACCCTGGCAGAGAGTTATGCCCAGTATGTTCACAACCTCTGCAACTCTCT CTCCATTAAAGTCGAGGAAAGTTATGCAATGCCAACCAAAACCATAGAAGTGTTGCAGTTGCAGGACCAA GGCAGCAAAATGCTCCTGGACTCAGTGCTTACCACCCATGAGCGAGTGGTTCAGATCAGCGGTTTGAGTG CTACGTTTGCAGAAATTTTCTTGGAAATAATCCAAAGCAGTCTTCCTGAAGGAGTCAGACTGTCAGTGAA GGAGCACACTGAAGAAGACTTCAAGGGACGATTCAAAGCTCGACCAGAACTGGAAGAACTGTTGGCCAAG TTGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203039 protein sequence
Red=Cloning site Green=Tags(s) MSGTLGKVLCLRNNTIFKQAFSLLRFRTSGEKPIYSVGGILLSISRPYKTKPTHGIGKYKHLIKAEEPKK KKGKVEVRAINLGTDYEYGVLNIHLTAYDMTLAESYAQYVHNLCNSLSIKVEESYAMPTKTIEVLQLQDQ GSKMLLDSVLTTHERVVQISGLSATFAEIFLEIIQSSLPEGVRLSVKEHTEEDFKGRFKARPELEELLAK LK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016055 |
ORF Size | 636 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016055.1, NM_016055.2, NM_016055.3, NM_016055.4, NM_016055.5, NP_057139.1 |
RefSeq Size | 997 bp |
RefSeq ORF | 639 bp |
Locus ID | 51642 |
MW | 23.9 kDa |
Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 6p. Several transcript variants, some protein-coding and some non-protein coding, have been found for this gene. [provided by RefSeq, Jan 2016] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC114521 | MRPL48 (untagged)-Human mitochondrial ribosomal protein L48 (MRPL48), nuclear gene encoding mitochondrial protein |
USD 420.00 |
|
RG203039 | MRPL48 (GFP-tagged) - Human mitochondrial ribosomal protein L48 (MRPL48), nuclear gene encoding mitochondrial protein |
USD 460.00 |
|
RC203039L3 | Lenti ORF clone of Human mitochondrial ribosomal protein L48 (MRPL48), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
USD 620.00 |
|
RC203039L4 | Lenti ORF clone of Human mitochondrial ribosomal protein L48 (MRPL48), nuclear gene encoding mitochondrial protein, mGFP tagged |
USD 620.00 |
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