Melanoma Inhibitory Activity (MIA) (NM_006533) Human Tagged ORF Clone
CAT#: RC203245
MIA (Myc-DDK-tagged)-Human melanoma inhibitory activity (MIA), transcript variant 1
"NM_006533" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MIA |
Synonyms | CD-RAP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203245 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGTCCCTGGTGTGCCTTGGTGTCATCATCTTGCTGTCTGCCTTCTCCGGACCTGGTGTCAGGG GTGGTCCTATGCCCAAGCTGGCTGACCGGAAGCTGTGTGCGGACCAGGAGTGCAGCCACCCTATCTCCAT GGCTGTGGCCCTTCAGGACTACATGGCCCCCGACTGCCGATTCCTGACCATTCACCGGGGCCAAGTGGTG TATGTCTTCTCCAAGCTGAAGGGCCGTGGGCGGCTCTTCTGGGGAGGCAGCGTTCAGGGAGATTACTATG GAGATCTGGCTGCTCGCCTGGGCTATTTCCCCAGTAGCATTGTCCGAGAGGACCAGACCCTGAAACCTGG CAAAGTCGATGTGAAGACAGACAAATGGGATTTCTACTGCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203245 protein sequence
Red=Cloning site Green=Tags(s) MARSLVCLGVIILLSAFSGPGVRGGPMPKLADRKLCADQECSHPISMAVALQDYMAPDCRFLTIHRGQVV YVFSKLKGRGRLFWGGSVQGDYYGDLAARLGYFPSSIVREDQTLKPGKVDVKTDKWDFYCQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006533 |
ORF Size | 393 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006533.1, NM_006533.2, NM_006533.3, NP_006524.1 |
RefSeq Size | 654 bp |
RefSeq ORF | 396 bp |
Locus ID | 8190 |
Domains | SH3 |
Protein Families | Secreted Protein, Transmembrane |
MW | 14.5 kDa |
Gene Summary | Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. [UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116021 | MIA (untagged)-Human melanoma inhibitory activity (MIA), transcript variant 1 |
USD 310.00 |
|
RG203245 | MIA (GFP-tagged) - Human melanoma inhibitory activity (MIA), transcript variant 1 |
USD 460.00 |
|
RC203245L1 | Lenti ORF clone of Human melanoma inhibitory activity (MIA), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC203245L2 | Lenti ORF clone of Human melanoma inhibitory activity (MIA), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC203245L3 | Lenti ORF clone of Human melanoma inhibitory activity (MIA), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC203245L4 | Lenti ORF clone of Human melanoma inhibitory activity (MIA), transcript variant 1, mGFP tagged |
USD 620.00 |
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