SMPX (NM_014332) Human Tagged ORF Clone

CAT#: RC203277

SMPX (Myc-DDK-tagged)-Human small muscle protein, X-linked (SMPX)

Product Images

Western blot validation of overexpression lysate (Cat# LY415310) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203277 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SMPX protein (Cat# TP303277). The protein was produced from HEK293T cells transfected with SMPX cDNA clone (Cat# RC203277) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMPX
• Synonyms: DFN6; DFNX4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203277 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATATGTCGAAACAGCCAGTTTCCAATGTTAGAGCCATCCAGGCAAATATCAATATTCCAATGGGAG
  CCTTTCGGCCAGGAGCAGGTCAACCCCCCAGAAGAAAAGAATGTACTCCTGAAGTGGAGGAGGGTGTTCC
  TCCCACCTCGGATGAGGAGAAGAAGCCAATTCCAGGAGCGAAGAAACTTCCAGGACCTGCAGTCAATCTA
  TCGGAAATCCAGAATATTAAAAGTGAACTAAAATATGTCCCCAAAGCTGAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203277 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNMSKQPVSNVRAIQANINIPMGAFRPGAGQPPRRKECTPEVEEGVPPTSDEEKKPIPGAKKLPGPAVNL
  SEIQNIKSELKYVPKAEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014332
• ORF Size: 264 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014332.1, NM_014332.2, NP_055147.1
• RefSeq Size: 951 bp
• RefSeq ORF: 267 bp
• Locus ID: 23676
• MW: 9.6 kDa
• Gene Summary: This gene encodes a small protein that has no known functional domains. Mutations in this gene are a cause of X-linked deafness-4, and the encoded protein may play a role in the maintenance of inner ear cells subjected to mechanical stress. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2011]