HSD17B2 (NM_002153) Human Tagged ORF Clone

CAT#: RC203293

HSD17B2 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 2 (HSD17B2)

Product Images

Western blot validation of overexpression lysate (Cat# LY419500) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203293 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HSD17B2 protein (Cat# TP303293). The protein was produced from HEK293T cells transfected with HSD17B2 cDNA clone (Cat# RC203293) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HSD17B2
• Synonyms: EDH17B2; HSD17; SDR9C2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203293 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACTTTCTTCTCGGACACAGCATGGATCTGCCTGGCTGTCCCCACAGTACTATGTGGGACAGTAT
  TTTGCAAATACAAGAAGAGCTCAGGGCAGCTGTGGAGCTGGATGGTCTGCCTGGCAGGCCTCTGTGCAGT
  CTGCCTGCTCATCCTGTCCCCTTTTTGGGGCTTGATCCTCTTCTCGGTGTCATGCTTCCTCATGTATACT
  TACTTATCTGGCCAAGAATTGTTACCTGTGGATCAGAAGGCAGTCCTGGTGACAGGTGGTGATTGCGGGC
  TTGGCCATGCTTTGTGCAAGTATCTGGATGAGCTGGGCTTCACGGTATTTGCCGGAGTTTTGAATGAAAA
  TGGCCCAGGAGCTGAGGAATTGCGAAGAACCTGCTCTCCGCGCCTCTCGGTGCTCCAAATGGACATCACG
  AAGCCAGTGCAGATAAAAGATGCTTACAGCAAGGTTGCAGCAATGCTGCAGGACAGAGGACTGTGGGCTG
  TGATCAACAATGCTGGGGTGCTTGGCTTTCCAACTGATGGGGAGCTTCTTCTTATGACTGACTACAAACA
  ATGCATGGCCGTGAACTTCTTTGGAACTGTGGAGGTCACAAAGACGTTTTTGCCTCTTCTTAGAAAATCC
  AAAGGGAGGCTGGTGAATGTCAGCAGCATGGGAGGAGGGGCCCCAATGGAAAGGCTGGCATCTTATGGCT
  CATCAAAGGCGGCTGTGACCATGTTCTCATCAGTTATGAGACTGGAGCTTTCCAAGTGGGGAATTAAAGT
  TGCTTCCATCCAACCTGGAGGCTTCCTAACAAATATCGCAGGCACCAGTGACAAGTGGGAAAAGCTGGAG
  AAGGACATTCTGGACCACCTCCCCGCTGAGGTACAGGAAGACTACGGCCAGGACTACATCTTAGCACAGC
  GGAATTTCCTCCTATTGATCAACTCGTTAGCCAGCAAGGACTTCTCTCCGGTGCTGCGGGACATCCAGCA
  TGCTATCTTGGCGAAGAGCCCTTTTGCCTATTACACGCCAGGGAAAGGCGCTTACTTGTGGATCTGCCTT
  GCTCACTATTTGCCTATTGGCATATATGATTACTTTGCTAAAAGACATTTTGGCCAAGACAAGCCCATGC
  CCAGAGCTCTAAGAATGCCTAACTACAAGAAAAAGGCCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203293 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTFFSDTAWICLAVPTVLCGTVFCKYKKSSGQLWSWMVCLAGLCAVCLLILSPFWGLILFSVSCFLMYT
  YLSGQELLPVDQKAVLVTGGDCGLGHALCKYLDELGFTVFAGVLNENGPGAEELRRTCSPRLSVLQMDIT
  KPVQIKDAYSKVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVTKTFLPLLRKS
  KGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLELSKWGIKVASIQPGGFLTNIAGTSDKWEKLE
  KDILDHLPAEVQEDYGQDYILAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICL
  AHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002153
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002153.1, NM_002153.2, NP_002144.1
• RefSeq Size: 1451 bp
• RefSeq ORF: 1164 bp
• Locus ID: 3294
• Cytogenetics: 16q23.3
• Domains: adh_short
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways
• MW: 42.8 kDa
• Gene Summary: ''