APIP (NM_015957) Human Tagged ORF Clone

CAT#: RC203297

APIP (Myc-DDK-tagged)-Human APAF1 interacting protein (APIP)

Product Images

Western blot validation of overexpression lysate (Cat# LY414309) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203297 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified APIP protein (Cat# TP303297). The protein was produced from HEK293T cells transfected with APIP cDNA clone (Cat# RC203297) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APIP
• Synonyms: APIP2; CGI-29; CGI29; hAPIP; MMRP19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203297 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGCTGTGATGCTCGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGACAAGGAGCGTC
  CAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGGGTCACTGGGACTGGAGGAGG
  AATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCTTCAGGAGTGCAAAAGGAACGAATTCAGCCT
  GAAGACATGTTTGTTTGTGATATAAATGAAAAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAA
  AAAGCCAGTGTACTCCTCTTTTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCA
  CTCTAAAGCTGCTGTGATGGCCACACTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG
  ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTGGTACCCATTA
  TTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCAGTGAATGAATACCCAGACTC
  CTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGGGGGGAAACATGGGAGAAGGCCAAAACCATG
  TGTGAGTGTTATGACTATTTATTTGATATTGCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGC
  TCCCAGTTGGAGAAAATGGAATTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203297 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGCDAREGDCCSRRCGAQDKERPRYLIPELCKQFYHLGWVTGTGGGISLKHGDEIYIAPSGVQKERIQP
  EDMFVCDINEKDISGPSPSKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGREFKITHQEM
  IKGIKKCTSGGYYRYDDMLVVPIIENTPEEKDLKDRMAHAVNEYPDSCAVLVRRHGVYVWGETWEKAKTM
  CECYDYLFDIAVSMKKVGLDPSQLPVGENGIV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015957
• ORF Size: 726 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015957.1, NM_015957.2, NM_015957.3, NP_057041.1
• RefSeq Size: 1285 bp
• RefSeq ORF: 729 bp
• Locus ID: 51074
• Domains: Aldolase_II
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• MW: 27.1 kDa
• Gene Summary: APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury (Cho et al., 2004 [PubMed 15262985]). [supplied by OMIM, Dec 2008]