N acetyl transferase 5 (NAA20) (NM_016100) Human Tagged ORF Clone
CAT#: RC203305
NAA20 (Myc-DDK-tagged)-Human N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 1
"NM_016100" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NAA20 |
Synonyms | dJ1002M8.1; NAT3; NAT3P; NAT5; NAT5P |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203305 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACGCTACGGGCCTTTACCTGCGACGACCTGTTCCGCTTCAACAACATTAACTTGGATCCACTTA CAGAAACTTATGGGATTCCTTTCTACCTACAATACCTCGCCCACTGGCCAGAGTATTTCATTGTTGCAGA GGCACCTGGTGGAGAATTAATGGGTTATATTATGGGTAAAGCAGAAGGCTCAGTAGCTAGGGAAGAATGG CACGGGCACGTCACAGCTCTGTCTGTTGCCCCAGAATTTCGACGCCTTGGTTTGGCTGCTAAACTTATGG AGTTACTAGAGGAGATTTCAGAAAGAAAGGGTGGATTTTTTGTGGATCTCTTTGTAAGAGTATCTAACCA AGTTGCAGTTAACATGTACAAGCAGTTGGGCTACAGTGTATATAGGACGGTCATAGAGTACTATTCGGCC AGCAACGGGGAGCCTGATGAGGACGCTTATGATATGAGGAAAGCACTTTCCAGGGATACTGAGAAGAAAT CCATCATACCATTACCTCATCCTGTGAGGCCTGAAGACATTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203305 protein sequence
Red=Cloning site Green=Tags(s) MTTLRAFTCDDLFRFNNINLDPLTETYGIPFYLQYLAHWPEYFIVAEAPGGELMGYIMGKAEGSVAREEW HGHVTALSVAPEFRRLGLAAKLMELLEEISERKGGFFVDLFVRVSNQVAVNMYKQLGYSVYRTVIEYYSA SNGEPDEDAYDMRKALSRDTEKKSIIPLPHPVRPEDIE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016100 |
ORF Size | 534 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016100.1, NM_016100.2, NM_016100.3, NM_016100.4, NP_057184.1 |
RefSeq Size | 1102 bp |
RefSeq ORF | 537 bp |
Locus ID | 51126 |
Domains | Acetyltransf |
Protein Pathways | Glycerophospholipid metabolism, Limonene and pinene degradation, Phenylalanine metabolism, Tyrosine metabolism |
MW | 20.4 kDa |
Gene Summary | NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]). [supplied by OMIM, Apr 2009] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC114456 | NAA20 (untagged)-Human N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 1 |
USD 310.00 |
|
RG203305 | NAA20 (GFP-tagged) - Human N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 1 |
USD 460.00 |
|
RC203305L1 | Lenti ORF clone of Human N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC203305L2 | Lenti ORF clone of Human N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC203305L3 | Lenti ORF clone of Human N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC203305L4 | Lenti ORF clone of Human N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 1, mGFP tagged |
USD 620.00 |
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