DPP3 (NM_130443) Human Tagged ORF Clone

CAT#: RC203406

DPP3 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 3 (DPP3), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY408959) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203406 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DPP3 protein (Cat# TP303406). The protein was produced from HEK293T cells transfected with DPP3 cDNA clone (Cat# RC203406) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPP3
• Synonyms: DPPIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203406 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACACCCAGTACATCCTGCCCAATGACATCGGCGTGTCTAGCCTGGACTGCCGTGAGGCCTTCC
  GCCTGCTGTCACCCACAGAGCGCCTCTATGCCTACCACCTGTCCCGTGCCGCCTGGTACGGAGGCCTGGC
  TGTGCTGCTTCAGACCTCCCCTGAGGCCCCCTACATCTATGCTCTGCTCAGCCGCCTCTTCCGCGCCCAG
  GACCCCGACCAGCTGCGCCAACATGCCCTGGCTGAAGGCCTTACCGAGGAGGAGTATCAGGCGTTCCTGG
  TCTATGCCGCGGGTGTTTACTCCAACATGGGCAACTACAAGTCCTTTGGTGACACCAAGTTTGTTCCCAA
  CTTGCCCAAGGAAAAGCTGGAACGGGTGATCCTAGGGAGTGAGGCTGCTCAGCAGCACCCAGAAGAAGTC
  AGGGGCCTCTGGCAGACCTGCGGGGAGCTTATGTTCTCTCTGGAGCCAAGGCTTCGACACCTCGGACTGG
  GGAAGGAGGGAATCACCACCTATTTCTCTGGGAATTGTACCATGGAAGATGCCAAATTGGCCCAGGACTT
  TCTGGACTCACAGAACCTCAGTGCCTACAACACCCGGCTCTTCAAAGAGGTCGATGGAGAAGGGAAGCCC
  TACTACGAGGTGCGGCTGGCTTCTGTGCTTGGCTCAGAGCCTTCCCTGGACTCTGAGGTGACTTCCAAGC
  TGAAGAGCTATGAATTCCGGGGAAGCCCTTTCCAGGTGACCCGGGGGGACTACGCGCCCATCCTCCAGAA
  GGTGGTGGAGCAGCTGGAGAAAGCCAAGGCCTATGCAGCCAACAGCCACCAGGGGCAGATGCTGGCCCAG
  TATATAGAGAGCTTCACCCAGGGCTCCATCGAGGCCCACAAGAGGGGCTCCCGCTTCTGGATCCAGGACA
  AAGGCCCCATCGTGGAGAGTTACATCGGGTTCATCGAGAGCTACCGCGACCCCTTTGGTTCCCGAGGAGA
  ATTTGAAGGTTTCGTAGCTGTGGTGAACAAGGCCATGAGTGCCAAGTTTGAGCGGCTGGTGGCGAGCGCA
  GAGCAGCTGCTGAAGGAGCTGCCCTGGCCCCCAACCTTTGAGAAGGACAAGTTCCTCACCCCTGACTTCA
  CCTCCCTGGATGTTCTCACCTTCGCTGGCTCCGGCATCCCTGCCGGCATCAACATCCCCAACTACGATGA
  TCTGAGGCAGACGGAAGGCTTTAAGAACGTGTCGCTGGGGAATGTGCTGGCTGTGGCCTACGCCACGCAG
  CGGGAGAAGCTTACCTTTCTGGAGGAGGATGACAAGGACCTGTACATCCTCTGGAAGGGGCCCTCCTTCG
  ATGTGCAGGTGGGCCTGCACGAGCTGCTGGGCCATGGCAGTGGCAAGCTCTTCGTACAGGACGAAAAAGG
  AGCATTCAACTTTGACCAGGAAACAGTGATCAACCCAGAGACGGGCGAGCAGATTCAGAGCTGGTATCGG
  AGCGGGGAGACCTGGGATAGCAAGTTCAGCACCATCGCCTCCAGCTACGAAGAGTGCCGGGCTGAGAGCG
  TGGGTCTCTACCTCTGTCTCCACCCGCAAGTGCTGGAGATCTTTGGCTTTGAGGGGGCTGATGCGGAGGA
  CGTGATCTACGTGAACTGGCTCAACATGGTTCGGGCCGGGCTGCTCGCTCTGGAGTTCTACACACCTGAG
  GCCTTCAACTGGCGACAGGCCCATATGCAGGCCCGGTTTGTGATCCTGAGAGTCTTGCTGGAGGCTGGCG
  AGGGACTCGTTACCATCACTCCCACCACAGGCTCCGATGGGCGCCCAGATGCCCGGGTCCGCCTCGACCG
  CAGCAAGATCCGGTCTGTGGGCAAGCCTGCTCTAGAGCGCTTCCTGCGGAGACTTCAGGTGCTGAAGTCC
  ACAGGGGATGTGGCCGGAGGGCGGGCCCTGTACGAGGGGTATGCAACGGTCACTGATGCGCCCCCCGAGT
  GCTTCCTCACCCTCAGGGACACGGTGCTGCTGCGTAAGGAATCTCGGAAGCTCATTGTTCAGCCCAACAC
  TCGCCTTGAAGGCTCAGACGTGCAGCTTCTGGAATACGAGGCGTCAGCTGCTGGCCTCATCCGATCCTTC
  TCTGAGCGTTTCCCAGAGGATGGACCCGAGTTGGAGGAGATCCTCACACAGCTGGCCACAGCCGATGCCC
  GATTCTGGAAGGGCCCCAGTGAGGCCCCATCTGGCCAAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203406 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADTQYILPNDIGVSSLDCREAFRLLSPTERLYAYHLSRAAWYGGLAVLLQTSPEAPYIYALLSRLFRAQ
  DPDQLRQHALAEGLTEEEYQAFLVYAAGVYSNMGNYKSFGDTKFVPNLPKEKLERVILGSEAAQQHPEEV
  RGLWQTCGELMFSLEPRLRHLGLGKEGITTYFSGNCTMEDAKLAQDFLDSQNLSAYNTRLFKEVDGEGKP
  YYEVRLASVLGSEPSLDSEVTSKLKSYEFRGSPFQVTRGDYAPILQKVVEQLEKAKAYAANSHQGQMLAQ
  YIESFTQGSIEAHKRGSRFWIQDKGPIVESYIGFIESYRDPFGSRGEFEGFVAVVNKAMSAKFERLVASA
  EQLLKELPWPPTFEKDKFLTPDFTSLDVLTFAGSGIPAGINIPNYDDLRQTEGFKNVSLGNVLAVAYATQ
  REKLTFLEEDDKDLYILWKGPSFDVQVGLHELLGHGSGKLFVQDEKGAFNFDQETVINPETGEQIQSWYR
  SGETWDSKFSTIASSYEECRAESVGLYLCLHPQVLEIFGFEGADAEDVIYVNWLNMVRAGLLALEFYTPE
  AFNWRQAHMQARFVILRVLLEAGEGLVTITPTTGSDGRPDARVRLDRSKIRSVGKPALERFLRRLQVLKS
  TGDVAGGRALYEGYATVTDAPPECFLTLRDTVLLRKESRKLIVQPNTRLEGSDVQLLEYEASAAGLIRSF
  SERFPEDGPELEEILTQLATADARFWKGPSEAPSGQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130443
• ORF Size: 2211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130443.2, NM_130443.3, NP_569710.2
• RefSeq Size: 3101 bp
• RefSeq ORF: 2214 bp
• Locus ID: 10072
• Domains: Peptidase_M49
• Protein Families: Druggable Genome, Protease
• MW: 82.6 kDa
• Gene Summary: This gene encodes a protein that is a member of the M49 family of metallopeptidases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2012]