CLC7 (CLCN7) (NM_001287) Human Tagged ORF Clone

CAT#: RC203450

CLCN7 (Myc-DDK-tagged)-Human chloride channel 7 (CLCN7), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY420027) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203450 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLCN7
• Synonyms: CLC-7; CLC7; OPTA2; OPTB4; PPP1R63
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203450 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACGTCTCTAAGAAGGTGTCCTGGTCCGGCCGGGACCGGGACGACGAGGAGGCGGCGCCGCTGC
  TGCGGAGGACGGCGCGGCCCGGCGGGGGGACGCCGCTGCTGAACGGGGCTGGGCCTGGGGCTGCGCGCCA
  GTCACCACGTTCTGCGCTTTTCCGAGTCGGACATATGAGCAGCGTGGAGCTGGATGATGAACTTTTGGAC
  CCGGATATGGACCCTCCACATCCCTTCCCCAAGGAGATCCCACACAACGAGAAGCTCCTGTCCCTCAAGT
  ATGAGAGCTTGGACTATGACAACAGTGAGAACCAGCTGTTCCTGGAGGAGGAGCGGCGGATCAATCACAC
  GGCCTTCCGGACGGTGGAGATCAAGCGCTGGGTCATCTGCGCCCTCATTGGGATCCTCACGGGCCTCGTG
  GCCTGCTTCATTGACATCGTGGTGGAAAACCTGGCTGGCCTCAAGTACAGGGTCATCAAGGGCAATATCG
  ACAAGTTCACAGAGAAGGGCGGACTGTCCTTCTCCCTGTTGCTGTGGGCCACGCTGAACGCCGCCTTCGT
  GCTCGTGGGCTCTGTGATTGTGGCTTTCATAGAGCCGGTGGCTGCTGGCAGCGGAATCCCCCAGATCAAG
  TGCTTCCTCAACGGGGTGAAGATCCCCCACGTGGTGCGGCTCAAGACGTTGGTGATCAAAGTGTCCGGTG
  TGATCCTGTCCGTGGTCGGGGGCCTGGCCGTGGGAAAGGAAGGGCCGATGATCCACTCAGGTTCAGTGAT
  TGCCGCCGGGATCTCTCAGGGAAGGTCAACGTCACTGAAACGAGATTTCAAGATCTTCGAGTACTTCCGC
  AGAGACACAGAGAAGCGGGACTTCGTCTCCGCAGGGGCTGCGGCCGGAGTGTCAGCGGCGTTTGGAGCCC
  CCGTGGGTGGGGTCCTGTTCAGCTTGGAGGAGGGTGCGTCCTTCTGGAACCAGTTCCTGACCTGGAGGAT
  CTTCTTTGCTTCCATGATCTCCACGTTCACCCTGAATTTTGTTCTGAGCATTTACCACGGGAACATGTGG
  GACCTGTCCAGCCCAGGCCTCATCAACTTCGGAAGGTTTGACTCGGAGAAAATGGCCTACACGATCCACG
  AGATCCCGGTCTTCATCGCCATGGGCGTGGTGGGCGGTGTGCTTGGAGCAGTGTTCAATGCCTTGAACTA
  CTGGCTGACCATGTTTCGAATCAGGTACATCCACCGGCCCTGCCTGCAGGTGATTGAGGCCGTGCTGGTG
  GCCGCCGTCACGGCCACAGTTGCCTTCGTGCTGATCTACTCGTCGCGGGATTGCCAGCCCCTGCAGGGGG
  GCTCCATGTCCTACCCGCTGCAGCTCTTTTGTGCAGATGGCGAGTACAACTCCATGGCTGCGGCCTTCTT
  CAACACCCCGGAGAAGAGCGTGGTGAGCCTCTTCCACGACCCGCCAGGCTCCTACAACCCCCTGACCCTC
  GGCCTGTTCACGCTGGTCTACTTCTTCCTGGCCTGCTGGACCTACGGGCTCACGGTGTCTGCCGGGGTCT
  TCATCCCGTCCCTGCTCATCGGGGCTGCCTGGGGCCGGCTCTTTGGGATCTCCCTGTCCTACCTCACGGG
  GGCGGCGATCTGGGCGGACCCCGGCAAATACGCCCTGATGGGAGCTGCTGCCCAGCTGGGCGGGATTGTG
  CGGATGACACTGAGCCTGACCGTCATCATGATGGAGGCCACCAGCAACGTGACCTACGGCTTCCCCATCA
  TGCTGGTGCTCATGACCGCCAAGATCGTGGGCGACGTCTTCATTGAGGGCCTGTACGACATGCACATTCA
  GCTGCAGAGTGTGCCCTTCCTGCACTGGGAGGCCCCGGTCACCTCACACTCACTCACTGCCAGGGAGGTG
  ATGAGCACACCAGTGACCTGCCTGAGGCGGCGTGAGAAGGTCGGCGTCATTGTGGACGTGCTGAGCGACA
  CGGCGTCCAATCACAACGGCTTCCCCGTGGTGGAGCATGCCGATGACACCCAGCCTGCCCGGCTCCAGGG
  CCTGATCCTGCGCTCCCAGCTCATCGTTCTCCTAAAGCACAAGGTGTTTGTGGAGCGGTCCAACCTGGGC
  CTGGTACAGCGGCGCCTGAGGCTGAAGGACTTCCGAGACGCCTACCCGCGCTTCCCACCCATCCAGTCCA
  TCCACGTGTCCCAGGACGAGCGGGAGTGCACCATGGACCTCTCCGAGTTCATGAACCCCTCCCCCTACAC
  GGTGCCCCAGGAGGCGTCGCTCCCACGGGTGTTCAAGCTGTTCCGGGCCCTGGGCCTGCGGCACCTGGTG
  GTGGTGGACAACCGCAATCAGGTTGTCGGGTTGGTGACCAGGAAGGACCTCGCCAGGTACCGCCTGGGAA
  AGAGAGGCTTGGAGGAGCTCTCGCTGGCCCAGACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203450 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANVSKKVSWSGRDRDDEEAAPLLRRTARPGGGTPLLNGAGPGAARQSPRSALFRVGHMSSVELDDELLD
  PDMDPPHPFPKEIPHNEKLLSLKYESLDYDNSENQLFLEEERRINHTAFRTVEIKRWVICALIGILTGLV
  ACFIDIVVENLAGLKYRVIKGNIDKFTEKGGLSFSLLLWATLNAAFVLVGSVIVAFIEPVAAGSGIPQIK
  CFLNGVKIPHVVRLKTLVIKVSGVILSVVGGLAVGKEGPMIHSGSVIAAGISQGRSTSLKRDFKIFEYFR
  RDTEKRDFVSAGAAAGVSAAFGAPVGGVLFSLEEGASFWNQFLTWRIFFASMISTFTLNFVLSIYHGNMW
  DLSSPGLINFGRFDSEKMAYTIHEIPVFIAMGVVGGVLGAVFNALNYWLTMFRIRYIHRPCLQVIEAVLV
  AAVTATVAFVLIYSSRDCQPLQGGSMSYPLQLFCADGEYNSMAAAFFNTPEKSVVSLFHDPPGSYNPLTL
  GLFTLVYFFLACWTYGLTVSAGVFIPSLLIGAAWGRLFGISLSYLTGAAIWADPGKYALMGAAAQLGGIV
  RMTLSLTVIMMEATSNVTYGFPIMLVLMTAKIVGDVFIEGLYDMHIQLQSVPFLHWEAPVTSHSLTAREV
  MSTPVTCLRRREKVGVIVDVLSDTASNHNGFPVVEHADDTQPARLQGLILRSQLIVLLKHKVFVERSNLG
  LVQRRLRLKDFRDAYPRFPPIQSIHVSQDERECTMDLSEFMNPSPYTVPQEASLPRVFKLFRALGLRHLV
  VVDNRNQVVGLVTRKDLARYRLGKRGLEELSLAQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287
• ORF Size: 2415 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287.1, NM_001287.2, NM_001287.3, NM_001287.4, NM_001287.5, NP_001278.1
• RefSeq Size: 4236 bp
• RefSeq ORF: 2418 bp
• Locus ID: 1186
• Cytogenetics: 16p13.3
• Domains: CBS, voltage_CLC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 88.7 kDa
• Gene Summary: 'The product of this gene belongs to the CLC chloride channel family of proteins. Chloride channels play important roles in the plasma membrane and in intracellular organelles. This gene encodes chloride channel 7. Defects in this gene are the cause of osteopetrosis autosomal recessive type 4 (OPTB4), also called infantile malignant osteopetrosis type 2 as well as the cause of autosomal dominant osteopetrosis type 2 (OPTA2), also called autosomal dominant Albers-Schonberg disease or marble disease autosoml dominant. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. OPTA2 is the most common form of osteopetrosis, occurring in adolescence or adulthood. [provided by RefSeq, Jul 2008]'