B MyB (MYBL2) (NM_002466) Human Tagged ORF Clone

CAT#: RC203540

MYBL2 (Myc-DDK-tagged)-Human v-myb myeloblastosis viral oncogene homolog (avian)-like 2 (MYBL2)

Product Images

Western blot validation of overexpression lysate (Cat# LY400875) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203540 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MYBL2 protein (Cat# TP303540). The protein was produced from HEK293T cells transfected with MYBL2 cDNA clone (Cat# RC203540) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MYBL2
• Synonyms: B-MYB; BMYB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203540 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGGCGGACGCGCTGCGAGGATCTGGATGAGCTGCACTACCAGGACACAGATTCAGATGTGCCGG
  AGCAGAGGGATAGCAAGTGCAAGGTCAAATGGACCCATGAGGAGGACGAGCAGCTGAGGGCCCTGGTGAG
  GCAGTTTGGACAGCAGGACTGGAAGTTCCTGGCCAGCCACTTCCCTAACCGCACTGACCAGCAATGCCAG
  TACAGGTGGCTGAGAGTTTTGAATCCAGACCTTGTCAAGGGGCCATGGACCAAAGAGGAAGACCAAAAAG
  TCATCGAGCTGGTTAAGAAGTATGGCACAAAGCAGTGGACACTGATTGCCAAGCACCTGAAGGGCCGGCT
  GGGGAAGCAGTGCCGTGAACGCTGGCACAACCACCTCAACCCTGAGGTGAAGAAGTCTTGCTGGACCGAG
  GAGGAGGACCGCATCATCTGCGAGGCCCACAAGGTGCTGGGCAACCGCTGGGCCGAGATCGCCAAGATGT
  TGCCAGGGAGGACAGACAATGCTGTGAAGAATCACTGGAACTCTACCATCAAAAGGAAGGTGGACACAGG
  AGGCTTCTTGAGCGAGTCCAAAGACTGCAAGCCCCCAGTGTACTTGCTGCTGGAGCTCGAGGACAAGGAC
  GGCCTCCAGAGTGCCCAGCCCACGGAAGGCCAGGGAAGTCTTCTGACCAACTGGCCCTCCGTCCCTCCTA
  CCATAAAGGAGGAGGAAAACAGTGAGGAGGAACTTGCAGCAGCCACCACATCGAAGGAACAGGAGCCCAT
  CGGTACAGATCTGGACGCAGTGCGAACACCAGAGCCCTTGGAGGAATTCCCGAAGCGTGAGGACCAGGAA
  GGCTCCCCACCAGAAACGAGCCTGCCTTACAAGTGGGTGGTGGAGGCAGCTAACCTCCTCATCCCCGCTG
  TGGGTTCTAGCCTCTCTGAAGCCCTGGACTTGATCGAGTCGGACCCTGATGCTTGGTGTGACCTGAGTAA
  ATTTGACCTCCCTGAGGAACCATCTGCAGAGGACAGTATCAACAACAGCCTAGTGCAGCTGCAAGCGTCA
  CATCAGCAGCAAGTCCTGCCACCCCGCCAGCCTTCCGCCCTGGTGCCCAGTGTGACCGAGTACCGCCTGG
  ATGGCCACACCATCTCAGACCTGAGCCGGAGCAGCCGGGGCGAGCTGATCCCCATCTCCCCCAGCACTGA
  AGTCGGGGGCTCTGGCATTGGCACACCGCCCTCTGTGCTCAAGCGGCAGAGGAAGAGGCGTGTGGCTCTG
  TCCCCTGTCACTGAGAATAGCACCAGTCTGTCCTTCCTGGATTCCTGTAACAGCCTCACGCCCAAGAGCA
  CACCTGTTAAGACCCTGCCCTTCTCGCCCTCCCAGTTTCTGAACTTCTGGAACAAACAGGACACATTGGA
  GCTGGAGAGCCCCTCGCTGACATCCACCCCAGTGTGCAGCCAGAAGGTGGTGGTCACCACACCACTGCAC
  CGGGACAAGACACCCCTGCACCAGAAACATGCTGCGTTTGTAACCCCAGATCAGAAGTACTCCATGGACA
  ACACTCCCCACACGCCAACCCCGTTCAAGAACGCCCTGGAGAAGTACGGACCCCTGAAGCCCCTGCCACA
  GACCCCGCACCTGGAGGAGGACTTGAAGGAGGTGCTGCGTTCTGAGGCTGGCATCGAACTCATCATCGAG
  GACGACATCAGGCCCGAGAAGCAGAAGAGGAAGCCTGGGCTGCGGCGGAGCCCCATCAAGAAAGTCCGGA
  AGTCTCTGGCTCTTGACATTGTGGATGAGGATGTGAAGCTGATGATGTCCACACTGCCCAAGTCTCTATC
  CTTGCCGACAACTGCCCCTTCAAACTCTTCCAGCCTCACCCTGTCAGGTATCAAAGAAGACAACAGCTTG
  CTCAACCAGGGCTTCTTGCAGGCCAAGCCCGAGAAGGCAGCAGTGGCCCAGAAGCCCCGAAGCCACTTCA
  CGACACCTGCCCCTATGTCCAGTGCCTGGAAGACGGTGGCCTGCGGGGGGACCAGGGACCAGCTTTTCAT
  GCAGGAGAAAGCCCGGCAGCTCCTGGGCCGCCTGAAGCCCAGCCACACATCTCGGACCCTCATCTTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203540 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRRTRCEDLDELHYQDTDSDVPEQRDSKCKVKWTHEEDEQLRALVRQFGQQDWKFLASHFPNRTDQQCQ
  YRWLRVLNPDLVKGPWTKEEDQKVIELVKKYGTKQWTLIAKHLKGRLGKQCRERWHNHLNPEVKKSCWTE
  EEDRIICEAHKVLGNRWAEIAKMLPGRTDNAVKNHWNSTIKRKVDTGGFLSESKDCKPPVYLLLELEDKD
  GLQSAQPTEGQGSLLTNWPSVPPTIKEEENSEEELAAATTSKEQEPIGTDLDAVRTPEPLEEFPKREDQE
  GSPPETSLPYKWVVEAANLLIPAVGSSLSEALDLIESDPDAWCDLSKFDLPEEPSAEDSINNSLVQLQAS
  HQQQVLPPRQPSALVPSVTEYRLDGHTISDLSRSSRGELIPISPSTEVGGSGIGTPPSVLKRQRKRRVAL
  SPVTENSTSLSFLDSCNSLTPKSTPVKTLPFSPSQFLNFWNKQDTLELESPSLTSTPVCSQKVVVTTPLH
  RDKTPLHQKHAAFVTPDQKYSMDNTPHTPTPFKNALEKYGPLKPLPQTPHLEEDLKEVLRSEAGIELIIE
  DDIRPEKQKRKPGLRRSPIKKVRKSLALDIVDEDVKLMMSTLPKSLSLPTTAPSNSSSLTLSGIKEDNSL
  LNQGFLQAKPEKAAVAQKPRSHFTTPAPMSSAWKTVACGGTRDQLFMQEKARQLLGRLKPSHTSRTLILS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002466
• ORF Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002466.1, NM_002466.2, NM_002466.3, NP_002457.1
• RefSeq Size: 2785 bp
• RefSeq ORF: 2103 bp
• Locus ID: 4605
• Cytogenetics: 20q13.12
• Domains: myb_DNA-binding
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• MW: 78.8 kDa
• Gene Summary: 'The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]'