HNRPLL (HNRNPLL) (NM_138394) Human Tagged ORF Clone

CAT#: RC203569

HNRPLL (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein L-like (HNRPLL), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY408658) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203569 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified HNRNPLL protein (Cat# TP303569). The protein was produced from HEK293T cells transfected with HNRNPLL cDNA clone (Cat# RC203569) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HNRNPLL
• Synonyms: HNRPLL; SRRF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203569 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCCTCCTCTTCCTCCCCCAGGGAGACGTACGAGGAGGACCGGGAGTACGAGAGCCAGGCCAAGC
  GTCTCAAGACCGAGGAGGGGGAGATCGACTACTCGGCCGAGGAAGGCGAGAACCGCCGGGAAGCGACGCC
  CCGGGGCGGGGGCGATGGCGGCGGCGGCGGCCGGAGCTTCTCTCAGCCGGAGGCAGGTGGAAGTCATCAT
  AAAGTTTCTGTTTCACCCGTCGTCCATGTTCGAGGACTCTGTGAATCTGTGGTGGAAGCAGACCTCGTGG
  AAGCGCTGGAAAAATTTGGGACAATATGCTATGTGATGATGATGCCATTTAAACGACAGGCTCTAGTGGA
  ATTTGAAAACATAGATAGTGCCAAAGAATGTGTGACATTTGCTGCAGATGAACCCGTGTACATTGCTGGT
  CAACAGGCTTTTTTCAACTATTCTACAAGCAAAAGGATCACTCGGCCAGGAAATACTGATGATCCATCAG
  GAGGCAACAAAGTTCTTCTGCTCTCAATTCAGAATCCGCTTTATCCAATTACAGTGGATGTTTTATATAC
  TGTATGCAACCCTGTTGGCAAAGTGCAACGTATTGTTATATTCAAGAGAAATGGGATACAAGCAATGGTT
  GAGTTTGAATCAGTCCTTTGTGCCCAGAAAGCTAAAGCAGCACTCAATGGAGCTGATATATATGCTGGAT
  GTTGCACACTAAAAATTGAATATGCACGGCCAACTCGTCTAAATGTTATTAGGAATGACAATGACAGTTG
  GGACTACACTAAACCATATTTGGGAAGACGAGATAGAGGAAAGGGTCGCCAGAGACAAGCCATTTTGGGA
  GAACACCCTTCTTCGTTTAGACATGATGGCTATGGATCCCATGGTCCATTATTGCCTTTACCAAGTCGTT
  ACAGAATGGGCTCTCGAGATACACCTGAACTTGTTGCTTATCCATTACCACAGGCTTCTTCCTCTTACAT
  GCATGGAGGAAATCCCTCTGGTTCAGTTGTAATGGTTAGTGGATTACATCAACTAAAAATGAATTGTTCA
  AGAGTCTTCAACCTGTTCTGCTTATATGGAAATATTGAGAAGGTAAAATTTATGAAGACCATTCCTGGTA
  CAGCACTGGTAGAAATGGGTGATGAGTATGCTGTAGAAAGAGCTGTCACACACCTTAATAATGTCAAATT
  ATTTGGGAAAAGACTTAATGTTTGCGTGTCTAAACAACATTCAGTTGTTCCAAGTCAAATATTTGAGCTG
  GAGGATGGTACCAGCAGCTACAAAGATTTTGCAATGAGCAAAAATAATCGCTTTACAAGTGCTGGCCAAG
  CATCTAAGAATATAATCCAGCCACCCTCCTGTGTTTTGCATTATTATAATGTTCCATTGTGTGTCACAGA
  AGAGACCTTCACAAAGTTGTGTAATGACCATGAAGTTCTTACATTCATCAAATATAAAGTGTTTGATGCA
  AAACCTTCAGCCAAAACACTTTCTGGGCTATTAGAATGGGAGTGCAAAACTGATGCAGTAGAAGCCCTTA
  CGGCACTGAATCACTATCAGATAAGAGTGCCGAATGGTTCCAATCCCTATACATTGAAGCTTTGCTTTTC
  TACATCATCCCATTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203569 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSSSSSPRETYEEDREYESQAKRLKTEEGEIDYSAEEGENRREATPRGGGDGGGGGRSFSQPEAGGSHH
  KVSVSPVVHVRGLCESVVEADLVEALEKFGTICYVMMMPFKRQALVEFENIDSAKECVTFAADEPVYIAG
  QQAFFNYSTSKRITRPGNTDDPSGGNKVLLLSIQNPLYPITVDVLYTVCNPVGKVQRIVIFKRNGIQAMV
  EFESVLCAQKAKAALNGADIYAGCCTLKIEYARPTRLNVIRNDNDSWDYTKPYLGRRDRGKGRQRQAILG
  EHPSSFRHDGYGSHGPLLPLPSRYRMGSRDTPELVAYPLPQASSSYMHGGNPSGSVVMVSGLHQLKMNCS
  RVFNLFCLYGNIEKVKFMKTIPGTALVEMGDEYAVERAVTHLNNVKLFGKRLNVCVSKQHSVVPSQIFEL
  EDGTSSYKDFAMSKNNRFTSAGQASKNIIQPPSCVLHYYNVPLCVTEETFTKLCNDHEVLTFIKYKVFDA
  KPSAKTLSGLLEWECKTDAVEALTALNHYQIRVPNGSNPYTLKLCFSTSSHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138394
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138394.2, NM_138394.3, NP_612403.2
• RefSeq Size: 3053 bp
• RefSeq ORF: 1629 bp
• Locus ID: 92906
• Domains: RRM
• MW: 60.1 kDa
• Gene Summary: HNRNPLL is a master regulator of activation-induced alternative splicing in T cells. In particular, it alters splicing of CD45 (PTPRC; MIM 151460), a tyrosine phosphatase essential for T-cell development and activation (Oberdoerffer et al., 2008 [PubMed 18669861]). [supplied by OMIM, Aug 2008]