WDR24 (NM_032259) Human Tagged ORF Clone

CAT#: RC203588

WDR24 (Myc-DDK-tagged)-Human WD repeat domain 24 (WDR24)

Product Images

Western blot validation of overexpression lysate (Cat# LY403152) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203588 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR24
• Synonyms: C16orf21; JFP7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203588 representing NM_032259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGATGTCCCGTGTGACCACAGCCCTGGGTGGCAGCGTGCTGACAGGCCGCACCATGCACTGCC
  ACCTGGATGCTCCCGCCAATGCCATCAGTGTGTGCCGCGACGCAGCCCAGGTGGTCGTGGCAGGCCGTAG
  CATCTTCAAGATCTATGCCATCGAGGAGGAACAGTTCGTGGAAAAGCTGAACCTGCGTGTGGGGCGCAAG
  CCTTCGCTTAACCTGAGCTGTGCTGACGTGGTCTGGCACCAGATGGATGAGAACCTGCTGGCCACAGCAG
  CCACCAATGGCGTGGTGGTCACGTGGAACCTGGGCCGGCCATCCCGCAACAAGCAGGACCAGCTGTTCAC
  AGAACACAAGCGCACGGTAAACAAAGTCTGCTTCCACCCCACCGAAGCCCACGTGCTGCTCAGTGGCTCC
  CAGGATGGCTTCATGAAGTGCTTTGACCTCCGCAGAAAGGACTCTGTCAGCACCTTCTCGGGCCAGTCGG
  AGAGCGTGCGGGACGTGCAGTTCAGTATCCGGGACTACTTCACCTTCGCCTCCACCTTTGAGAACGGCAA
  TGTGCAGCTCTGGGACATCCGGCGTCCCGACCGGTGCGAGAGGATGTTCACAGCCCACAACGGACCCGTC
  TTCTGCTGCGACTGGCACCCCGAGGACAGGGGCTGGTTGGCCACTGGAGGGCGCGACAAGATGGTGAAGG
  TCTGGGACATGACCACGCACCGTGCCAAGGAGATGCACTGTGTGCAGACCATCGCCTCGGTGGCCCGTGT
  GAAGTGGCGGCCAGAGTGCCGCCACCACCTGGCCACGTGCTCCATGATGGTGGACCACAACATCTATGTT
  TGGGACGTGCGCCGGCCCTTCGTGCCAGCTGCCATGTTTGAGGAACACCGAGACGTCACCACGGGAATTG
  CCTGGCGCCACCCCCACGACCCCTCCTTCCTGCTGTCTGGCTCCAAGGACAGCTCGCTGTGCCAGCACCT
  GTTCCGCGACGCCAGCCAGCCCGTCGAGCGCGCCAACCCTGAGGGCCTCTGCTACGGCCTCTTCGGGGAC
  CTGGCCTTCGCCGCCAAGGAGAGCCTCGTGGCTGCCGAGTCGGGGCGCAAGCCCTACACTGGCGACCGGC
  GCCACCCCATCTTCTTTAAGCGCAAGCTGGACCCTGCCGAGCCCTTCGCAGGCCTCGCCTCCAGTGCCCT
  CAGTGTCTTTGAGACGGAGCCAGGTGGCGGCGGCATGCGCTGGTTTGTGGACACAGCTGAGCGTTATGCG
  CTGGCTGGCCGGCCACTGGCCGAGCTCTGTGACCACAACGCAAAGGTGGCTCGAGAGCTTGGCCGCAACC
  AGGTGGCGCAAACGTGGACCATGCTGCGGATCATCTACTGCAGCCCTGGCCTAGTGCCCACTGCAAACCT
  CAACCACAGTGTGGGCAAGGGTGGCTCCTGTGGCCTCCCGCTCATGAACAGTTTCAACCTGAAGGATATG
  GCCCCAGGGTTGGGCAGTGAGACGCGGCTGGACCGCAGCAAAGGAGATGCACGGAGCGACACAGTTCTGC
  TCGACTCCTCGGCCACACTCATCACCAATGAGGATAACGAGGAAACCGAGGGCAGCGACGTACCTGCCGA
  CTACCTGCTGGGTGACGTGGAAGGTGAGGAGGACGAGCTGTACCTGCTGGATCCGGAACACGCGCACCCC
  GAGGACCCTGAGTGCGTGCTGCCGCAGGAGGCCTTTCCGCTGCGCCACGAGATCGTGGACACGCCTCCCG
  GACCCGAGCACCTGCAGGACAAGGCCGACTCCCCGCACGTGAGCGGCAGCGAGGCGGATGTGGCCTCCCT
  GGCCCCCGTGGACTCCTCCTTCTCGCTCCTGTCTGTCTCACACGCGCTCTACGACAGCCGCCTGCCGCCC
  GACTTCTTCGGCGTGCTGGTGCGCGACATGCTGCACTTCTACGCTGAGCAGGGCGACGTGCAGATGGCTG
  TGTCTGTGCTCATCGTCCTGGGTGAACGGGTGCGCAAGGACATCGACGAGCAGACCCAGGAGCACTGGTA
  CACTTCCTACATCGACCTGCTGCAGCGCTTCCGCCTCTGGAACGTGTCCAACGAGGTGGTCAAGCTGAGC
  ACCAGCCGCGCCGTCAGCTGCCTCAACCAGGCCTCCACCACCCTGCACGTCAACTGCAGCCACTGCAAGC
  GGCCCATGAGCAGCCGGGGCTGGGTCTGCGACAGGTGCCACCGCTGCGCCAGCATGTGTGCCGTCTGCCA
  CCACGTAGTCAAGGGTCTCTTCGTGTGGTGCCAGGGCTGCAGCCACGGCGGCCACCTGCAGCACATCATG
  AAGTGGCTGGAAGGCAGCTCCCACTGTCCCGCAGGCTGCGGCCACCTCTGCGAGTACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203588 representing NM_032259
  Red=Cloning site Green=Tags(s)
  
  MEKMSRVTTALGGSVLTGRTMHCHLDAPANAISVCRDAAQVVVAGRSIFKIYAIEEEQFVEKLNLRVGRK
  PSLNLSCADVVWHQMDENLLATAATNGVVVTWNLGRPSRNKQDQLFTEHKRTVNKVCFHPTEAHVLLSGS
  QDGFMKCFDLRRKDSVSTFSGQSESVRDVQFSIRDYFTFASTFENGNVQLWDIRRPDRCERMFTAHNGPV
  FCCDWHPEDRGWLATGGRDKMVKVWDMTTHRAKEMHCVQTIASVARVKWRPECRHHLATCSMMVDHNIYV
  WDVRRPFVPAAMFEEHRDVTTGIAWRHPHDPSFLLSGSKDSSLCQHLFRDASQPVERANPEGLCYGLFGD
  LAFAAKESLVAAESGRKPYTGDRRHPIFFKRKLDPAEPFAGLASSALSVFETEPGGGGMRWFVDTAERYA
  LAGRPLAELCDHNAKVARELGRNQVAQTWTMLRIIYCSPGLVPTANLNHSVGKGGSCGLPLMNSFNLKDM
  APGLGSETRLDRSKGDARSDTVLLDSSATLITNEDNEETEGSDVPADYLLGDVEGEEDELYLLDPEHAHP
  EDPECVLPQEAFPLRHEIVDTPPGPEHLQDKADSPHVSGSEADVASLAPVDSSFSLLSVSHALYDSRLPP
  DFFGVLVRDMLHFYAEQGDVQMAVSVLIVLGERVRKDIDEQTQEHWYTSYIDLLQRFRLWNVSNEVVKLS
  TSRAVSCLNQASTTLHVNCSHCKRPMSSRGWVCDRCHRCASMCAVCHHVVKGLFVWCQGCSHGGHLQHIM
  KWLEGSSHCPAGCGHLCEYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032259
• ORF Size: 2370 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032259.1, NM_032259.2, NM_032259.3, NP_115635.1
• RefSeq Size: 3165
• RefSeq ORF: 2373
• Locus ID: 84219
• Domains: WD40
• Protein Families: Druggable Genome
• MW: 88.7 kDa