IFT43 (NM_052873) Human Tagged ORF Clone
CAT#: RC203591
IFT43 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 179 (C14orf179), transcript variant 1
"NM_052873" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | IFT43 |
Synonyms | C14orf179; CED3; RP81; SRTD18 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203591 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATTTGCTCGACTTGGACGAGGAGCTTCGCTACAGCTTGGCTACCTCCAGGGCCAAGATGGGTC GCCGAGCTCAACAGGAGTCAGCGCAGGCCGAGAATCACCTCAATGGCAAGAATTCCTCTTTGACTCTGAC TGGAGAGACTTCCTCTGCTAAATTACCTCGCTGCCGACAGGGAGGCTGGGCAGGTGATTCCGTGAAGGCT TCGAACGGTACCCAAACAGGCAAACAACAGCTGGATCTGAACGCATGCTATCACAAAACGCATCACAGAA ATTTGGGGCTGGCTTCATTGGAAGAGGCAGATATTCCTATCATTCCGGATCTGGAGGAAGTACAGGAAGA AGACTTTGTTTTGCAGGTGGCAGCCCCTCCCAGCATCCAGATAAAGCGGGTGATGACCTACCGTGACCTG GACAATGACCTCATGAAGTACTCAGCCATTCAGACACTGGATGGGGAGATCGACCTGAAACTCCTCACCA AAGTGCTCGCGCCGGAGCACGAAGTCCGGGAGGATGATGTCGGCTGGGACTGGGACCATCTGTTCACTGA GGTGTCCTCAGAGGTCCTCACTGAGTGGGACCCACTGCAGACGGAGAAGGAGGACCCTGCGGGGCAGGCC AGGCACACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203591 protein sequence
Red=Cloning site Green=Tags(s) MEDLLDLDEELRYSLATSRAKMGRRAQQESAQAENHLNGKNSSLTLTGETSSAKLPRCRQGGWAGDSVKA SNGTQTGKQQLDLNACYHKTHHRNLGLASLEEADIPIIPDLEEVQEEDFVLQVAAPPSIQIKRVMTYRDL DNDLMKYSAIQTLDGEIDLKLLTKVLAPEHEVREDDVGWDWDHLFTEVSSEVLTEWDPLQTEKEDPAGQA RHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_052873 |
ORF Size | 639 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_052873.1, NM_052873.2, NP_443105.1 |
RefSeq Size | 865 bp |
RefSeq ORF | 642 bp |
Locus ID | 112752 |
MW | 23.9 kDa |
Gene Summary | This gene encodes a subunit of the intraflagellar transport complex A (IFT-A). IFT-A is a multiprotein complex that plays an important role in cilia assembly and maintenance by mediating retrograde ciliary transport. Mutations in this gene are a cause of cranioectodermal dysplasia-3 (CED3), also known as Sensenbrenner syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC120155 | IFT43 (untagged)-Human chromosome 14 open reading frame 179 (C14orf179), transcript variant 1 |
USD 420.00 |
|
RG203591 | IFT43 (GFP-tagged) - Human chromosome 14 open reading frame 179 (C14orf179), transcript variant 1 |
USD 460.00 |
|
RC203591L1 | Lenti ORF clone of Human chromosome 14 open reading frame 179 (C14orf179), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC203591L2 | Lenti ORF clone of Human chromosome 14 open reading frame 179 (C14orf179), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC203591L3 | Lenti ORF clone of Human chromosome 14 open reading frame 179 (C14orf179), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC203591L4 | Lenti ORF clone of Human chromosome 14 open reading frame 179 (C14orf179), transcript variant 1, mGFP tagged |
USD 620.00 |
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