RXYLT1 (NM_014254) Human Tagged ORF Clone

CAT#: RC203762

TMEM5 (Myc-DDK-tagged)-Human transmembrane protein 5 (TMEM5)

Product Images

Western blot validation of overexpression lysate (Cat# LY415410) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203762 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RXYLT1 protein (Cat# TP303762). The protein was produced from HEK293T cells transfected with RXYLT1 cDNA clone (Cat# RC203762) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RXYLT1
• Synonyms: HP10481; MDDGA10; TMEM5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203762 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTGACGCGGAAGCGGCTCTGCTCGTTTCTTATCGCCCTGTACTGCCTATTCTCCCTCTACGCTG
  CCTACCACGTCTTCTTCGGGCGCCGCCGCCAGGCGCCGGCCGGGTCCCCGCGGGGCCTCAGGAAGGGGGC
  GGCCCCCGCGCGGGAGAGACGCGGCCGAGAACAGTCCACTTTGGAAAGTGAAGAATGGAATCCTTGGGAA
  GGAGATGAAAAAAATGAGCAACAACACAGATTTAAAACTAGCCTTCAAATATTAGATAAATCCACGAAAG
  GAAAAACAGATCTCAGTGTACAAATCTGGGGCAAAGCTGCCATTGGCTTGTATCTCTGGGAGCATATTTT
  TGAAGGCTTACTTGATCCCAGCGATGTGACTGCTCAATGGAGAGAAGGAAAGTCAATCGTAGGAAGAACA
  CAGTACAGCTTCATCACTGGTCCAGCTGTAATACCAGGGTACTTCTCCGTTGATGTGAATAATGTGGTAC
  TCATTTTAAATGGAAGAGAAAAAGCAAAGATCTTTTATGCCACCCAGTGGTTACTTTATGCACAAAATTT
  AGTGCAAATTCAAAAACTCCAGCATCTTGCTGTTGTTTTGCTCGGAAATGAACATTGTGATAATGAGTGG
  ATAAACCCATTCCTCAAAAGAAATGGAGGCTTCGTGGAGCTGCTTTTCATAATATATGACAGCCCCTGGA
  TTAATGACGTGGATGTTTTTCAGTGGCCTTTAGGAGTAGCAACATACAGGAATTTTCCTGTGGTGGAGGC
  AAGTTGGTCAATGCTGCATGATGAGAGGCCATATTTATGTAATTTCTTAGGAACGATTTATGAAAATTCA
  TCCAGACAGGCACTAATGAACATTTTGAAAAAAGATGGGAACGATAAGCTTTGTTGGGTTTCAGCAAGAG
  AACACTGGCAGCCTCAGGAAACAAATGAAAGTCTTAAGAATTACCAAGATGCCTTGCTTCAGAGTGATCT
  CACATTGTGCCCGGTCGGAGTAAACACAGAATGCTATCGAATCTATGAGGCTTGCTCCTATGGCTCCATT
  CCTGTGGTGGAAGACGTGATGACAGCTGGCAACTGTGGGAATACATCTGTGCACCACGGTGCTCCTCTGC
  AGTTACTCAAGTCCATGGGTGCTCCCTTTATCTTTATCAAGAACTGGAAGGAACTCCCTGCTGTTTTAGA
  AAAAGAGAAAACTATAATTTTACAAGAAAAAATTGAAAGAAGAAAAATGTTACTTCAGTGGTATCAGCAC
  TTCAAGACAGAGCTTAAAATGAAATTTACTAATATTTTAGAAAGCTCATTTTTAATGAATAATAAAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203762 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRLTRKRLCSFLIALYCLFSLYAAYHVFFGRRRQAPAGSPRGLRKGAAPARERRGREQSTLESEEWNPWE
  GDEKNEQQHRFKTSLQILDKSTKGKTDLSVQIWGKAAIGLYLWEHIFEGLLDPSDVTAQWREGKSIVGRT
  QYSFITGPAVIPGYFSVDVNNVVLILNGREKAKIFYATQWLLYAQNLVQIQKLQHLAVVLLGNEHCDNEW
  INPFLKRNGGFVELLFIIYDSPWINDVDVFQWPLGVATYRNFPVVEASWSMLHDERPYLCNFLGTIYENS
  SRQALMNILKKDGNDKLCWVSAREHWQPQETNESLKNYQDALLQSDLTLCPVGVNTECYRIYEACSYGSI
  PVVEDVMTAGNCGNTSVHHGAPLQLLKSMGAPFIFIKNWKELPAVLEKEKTIILQEKIERRKMLLQWYQH
  FKTELKMKFTNILESSFLMNNKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014254
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014254.1, NM_014254.2, NP_055069.1
• RefSeq Size: 1963 bp
• RefSeq ORF: 1332 bp
• Locus ID: 10329
• Protein Families: Transmembrane
• MW: 51.1 kDa
• Gene Summary: This gene encodes a type II transmembrane protein that is thought to have glycosyltransferase function. Mutations in this gene result in cobblestone lissencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]