Granuphilin (SYTL4) (NM_080737) Human Tagged ORF Clone

CAT#: RC203842

SYTL4 (Myc-DDK-tagged)-Human synaptotagmin-like 4 (SYTL4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427077) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226081 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SYTL4
• Synonyms: SLP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203842 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAGTTACTGGACCTTTCTTTTCTGTCTGAGGAGGAAAAGGATTTGATTCTCAGTGTTCTACAGC
  GAGATGAAGAGGTCCGGAAAGCAGATGAGAAAAGGATTAGGCGACTAAAGAATGAGTTACTGGAGATAAA
  AAGGAAAGGGGCCAAGAGGGGCAGCCAACACTACAGTGATCGGACCTGTGCCCGGTGCCAGGAGAGCCTG
  GGCCGTTTGAGTCCCAAAACCAATACTTGTCGGGGTTGTAATCACCTGGTGTGTCGGGACTGCCGCATAC
  AGGAAAGCAATGGTACCTGGAGGTGCAAGGTGTGCGCCAAGGAAATAGAGTTGAAGAAAGCAACTGGGGA
  CTGGTTTTATGACCAGAAAGTGAATCGCTTTGCTTACCGCACAGGTAGTGAGATAATCAGGATGTCCCTG
  CGCCACAAACCTGCAGTGAGTAAAAGAGAGACAGTGGGACAGTCCCTCCTTCATCAGACACAGATGGGTG
  ACATCTGGCCAGGAAGAAAGATCATTCAGGAGCGGCAGAAGGAGCCCAGTGTGCTATTTGAAGTGCCAAA
  GCTGAAAAGTGGAAAGAGTGCATTGGAAGCTGAGAGTGAGAGTCTGGATAGCTTCACAGCTGACTCGGAT
  AGCACCTCCAGGAGAGACTCTCTGGATAAATCTGGCCTCTTTCCAGAATGGAAGAAGATGTCTGCTCCCA
  AATCTCAAGTAGAAAAGGAAACTCAGCCTGGAGGTCAAAATGTGGTATTTGTGGATGAGGGTGAGATGAT
  ATTTAAGAAGAACACCAGAAAAATCCTCAGGCCTTCAGAGTACACTAAATCTGTGATAGATCTTCGCCCA
  GAAGATGTGGTACATGAAAGTGGCTCCTTGGGAGACAGAAGCAAATCCGTCCCAGGCCTCAATGTGGATA
  TGGAAGAGGAAGAAGAAGAAGAAGACATTGACCACCTAGTGAAGTTACATCGCCAGAAGCTAGCCAGAAG
  CAGCATGCAAAGTGGCTCCTCCATGAGTACGATCGGCAGCATGATGAGCATCTACAGTGAAGCTGGTGAT
  TTCGGGAACATCTTTGTGACTGGCAGGATTGCCTTTTCCCTGAAGTATGAGCAGCAAACCCAGAGTCTGG
  TTGTCCATGTGAAGGAGTGCCATCAGCTGGCCTATGCTGATGAAGCCAAGAAGCGCTCTAACCCATATGT
  GAAGACTTACCTTCTGCCTGACAAGTCCCGCCAAGGAAAAAGAAAAACCAGCATCAAGCGGGACACTGTT
  AATCCACTATATGATGAGACGCTGAGGTATGAGATCCCAGAATCTCTCCTGGCCCAGAGGACCCTGCAGT
  TCTCAGTTTGGCATCATGGTCGTTTTGGCAGAAACACTTTCCTTGGAGAGGCAGAGATCCAGATGGATTC
  CTGGAAGCTTGATAAGAAACTGGATCATTGCCTCCCTTTACATGGAAAGATCAGTGCTGAGTCCCCGACT
  GGCTTGCCATCACACAAAGGCGAGTTGGTGGTTTCATTGAAATACATCCCAGCCTCCAAAACCCCTGTTG
  GAGGTGACCGGAAAAAGAGTAAAGGTGGGGAAGGGGGAGAGCTCCAGGTGTGGATCAAAGAAGCCAAGAA
  CTTGACGGCTGCCAAAGCAGGAGGGACTTCAGACAGCTTTGTCAAGGGATACCTCCTTCCCATGAGGAAC
  AAGGCCAGTAAACGTAAAACTCCTGTGATGAAGAAGACCCTGAATCCTCACTACAACCATACATTTGTCT
  ACAATGGTGTGAGGCTGGAAGATCTACAGCATATGTGCCTGGAACTGACTGTGTGGGACCGGGAGCCCCT
  GGCCAGCAATGACTTCCTGGGAGGGGTCAGGCTGGGTGTTGGCACTGGGATCAGTAATGGGGAAGTGGTG
  GACTGGATGGACTCGACTGGGGAAGAAGTGAGCCTGTGGCAGAAGATGCGACAGTACCCAGGGTCTTGGG
  CAGAAGGGACTCTGCAGCTCCGTTCCTCAATGGCCAAGCAGAAGCTGGGTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203842 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSELLDLSFLSEEEKDLILSVLQRDEEVRKADEKRIRRLKNELLEIKRKGAKRGSQHYSDRTCARCQESL
  GRLSPKTNTCRGCNHLVCRDCRIQESNGTWRCKVCAKEIELKKATGDWFYDQKVNRFAYRTGSEIIRMSL
  RHKPAVSKRETVGQSLLHQTQMGDIWPGRKIIQERQKEPSVLFEVPKLKSGKSALEAESESLDSFTADSD
  STSRRDSLDKSGLFPEWKKMSAPKSQVEKETQPGGQNVVFVDEGEMIFKKNTRKILRPSEYTKSVIDLRP
  EDVVHESGSLGDRSKSVPGLNVDMEEEEEEEDIDHLVKLHRQKLARSSMQSGSSMSTIGSMMSIYSEAGD
  FGNIFVTGRIAFSLKYEQQTQSLVVHVKECHQLAYADEAKKRSNPYVKTYLLPDKSRQGKRKTSIKRDTV
  NPLYDETLRYEIPESLLAQRTLQFSVWHHGRFGRNTFLGEAEIQMDSWKLDKKLDHCLPLHGKISAESPT
  GLPSHKGELVVSLKYIPASKTPVGGDRKKSKGGEGGELQVWIKEAKNLTAAKAGGTSDSFVKGYLLPMRN
  KASKRKTPVMKKTLNPHYNHTFVYNGVRLEDLQHMCLELTVWDREPLASNDFLGGVRLGVGTGISNGEVV
  DWMDSTGEEVSLWQKMRQYPGSWAEGTLQLRSSMAKQKLGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080737
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080737.1, NM_080737.2, NP_542775.1
• RefSeq Size: 3936 bp
• RefSeq ORF: 2016 bp
• Locus ID: 94121
• Domains: C2, RPH3A_effector
• MW: 76 kDa
• Gene Summary: This gene encodes a member of the synaptotagmin like protein family. Members of this family are characterized by an N-terminal Rab27 binding domain and C-terminal tandem C2 domains. The encoded protein binds specific small Rab GTPases and is involved in intracellular membrane trafficking. This protein binds Rab27 and may be involved in inhibiting dense core vesicle exocytosis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Mar 2010]