MAGOH (NM_002370) Human Tagged ORF Clone

CAT#: RC203863

MAGOH (Myc-DDK-tagged)-Human mago-nashi homolog, proliferation-associated (Drosophila) (MAGOH)

Product Images

Western blot validation of overexpression lysate (Cat# LY419380) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203863 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAGOH
• Synonyms: MAGOH1; MAGOHA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203863 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGTGACTTTTATCTGCGTTACTACGTGGGGCACAAGGGCAAGTTCGGCCACGAGTTCCTGGAGT
  TTGAGTTTCGACCGGACGGGAAGTTAAGATATGCCAACAACAGCAATTACAAGAATGATGTCATGATCAG
  AAAAGAGGCTTATGTACATAAAAGCGTGATGGAGGAACTGAAGAGAATAATTGACGACAGTGAAATTACC
  AAAGAGGATGATGCATTGTGGCCTCCTCCTGACCGAGTGGGCCGGCAGGAGCTTGAAATCGTCATTGGAG
  ATGAACACATTTCTTTTACAACATCAAAAATTGGTTCCCTTATTGATGTCAATCAATCCAAGGATCCAGA
  AGGCTTACGAGTATTTTATTATCTTGTCCAGGACCTGAAGTGTTTGGTCTTCAGTCTTATTGGATTACAC
  TTCAAGATTAAACCAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203863 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVMEELKRIIDDSEIT
  KEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSKDPEGLRVFYYLVQDLKCLVFSLIGLH
  FKIKPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002370
• ORF Size: 438 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002370.1, NM_002370.2, NM_002370.3, NP_002361.1
• RefSeq Size: 772 bp
• RefSeq ORF: 441 bp
• Locus ID: 4116
• Cytogenetics: 1p32.3
• Domains: Mago_nashi
• Protein Pathways: Spliceosome
• MW: 17.2 kDa
• Gene Summary: 'Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq, Jul 2008]'