WBP2 (NM_012478) Human Tagged ORF Clone

CAT#: RC203955

WBP2 (Myc-DDK-tagged)-Human WW domain binding protein 2 (WBP2)

Product Images

Western blot validation of overexpression lysate (Cat# LY402223) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203955 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WBP2
• Synonyms: DFNB107; GRAMD6; WBP-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203955 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTCAACAAGAATCACTCGGAGGGCGGCGGAGTGATCGTCAATAACACCGAGAGCATCCTAATGT
  CCTATGATCACGTGGAACTCACATTCAATGACATGAAGAACGTGCCAGAAGCCTTCAAAGGGACCAAGAA
  AGGCACTGTCTACCTTACCCCTTACCGGGTCATCTTTCTGTCCAAGGGCAAGGATGCCATGCAGTCCTTC
  ATGATGCCATTTTATCTCATGAAAGACTGTGAGATCAAGCAGCCCGTATTTGGTGCAAACTACATCAAGG
  GAACAGTGAAGGCGGAAGCGGGAGGTGGCTGGGAAGGCTCTGCTTCCTACAAGTTGACTTTCACGGCAGG
  GGGCGCCATTGAGTTCGGACAGCGGATGCTCCAGGTGGCATCTCAAGCCTCCAGAGGTGAAGTCCCCAGT
  GGAGCCTATGGCTACTCTTACATGCCCAGCGGGGCCTATGTCTATCCCCCGCCAGTCGCCAATGGAATGT
  ACCCCTGCCCTCCTGGCTACCCCTATCCACCGCCCCCACCTGAGTTCTATCCAGGACCCCCCATGATGGA
  CGGGGCCATGGGATACGTGCAGCCCCCACCACCGCCCTACCCTGGGCCCATGGAACCTCCGGTCAGCGGC
  CCCGATGTCCCCTCCACTCCTGCAGCCGAAGCCAAGGCCGCAGAAGCAGCCGCCAGCGCCTATTACAACC
  CAGGCAATCCTCACAACGTCTACATGCCCACGAGCCAGCCGCCGCCACCTCCCTACTACCCACCGGAAGA
  TAAGAAGACCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203955 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALNKNHSEGGGVIVNNTESILMSYDHVELTFNDMKNVPEAFKGTKKGTVYLTPYRVIFLSKGKDAMQSF
  MMPFYLMKDCEIKQPVFGANYIKGTVKAEAGGGWEGSASYKLTFTAGGAIEFGQRMLQVASQASRGEVPS
  GAYGYSYMPSGAYVYPPPVANGMYPCPPGYPYPPPPPEFYPGPPMMDGAMGYVQPPPPPYPGPMEPPVSG
  PDVPSTPAAEAKAAEAAASAYYNPGNPHNVYMPTSQPPPPPYYPPEDKKTQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012478
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012478.2, NM_012478.3, NP_036610.2
• RefSeq Size: 1944 bp
• RefSeq ORF: 786 bp
• Locus ID: 23558
• Domains: GRAM
• MW: 28.1 kDa
• Gene Summary: The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. This gene encodes a WW domain binding protein that is a transcriptional coactivator of estrogen receptor alpha and progesterone receptor. Defects in this gene have been associated with hearing impairment. [provided by RefSeq, Jan 2017]