PGM2 (NM_018290) Human Tagged ORF Clone

CAT#: RC203979

PGM2 (Myc-DDK-tagged)-Human phosphoglucomutase 2 (PGM2)

Product Images

Western blot validation of overexpression lysate (Cat# LY413138) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203979 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PGM2 protein (Cat# TP303979). The protein was produced from HEK293T cells transfected with PGM2 cDNA clone (Cat# RC203979) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PGM2
• Synonyms: MSTP006
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203979 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTCCAGAAGGCAGCGGTCTAGACGAGGACGCCCGGCTGGACCAGGAGACCGCCCAGTGGCTGC
  GCTGGGACAAGAATTCCTTAACTTTGGAGGCAGTGAAACGACTAATAGCAGAAGGTAATAAAGAAGAACT
  ACGAAAATGTTTTGGGGCCCGAATGGAGTTTGGGACAGCTGGCCTCCGAGCTGCTATGGGACCTGGAATT
  TCTCGTATGAATGACTTGACCATCATCCAGACTACACAGGGATTTTGCAGATACCTGGAAAAACAATTCA
  GTGACTTAAAGCAGAAAGGCATCGTGATCAGTTTTGACGCCCGAGCTCATCCATCCAGTGGGGGTAGCAG
  CAGAAGGTTTGCCCGACTTGCTGCAACCACATTTATCAGTCAGGGGATTCCTGTGTACCTCTTTTCTGAT
  ATAACGCCAACCCCCTTTGTGCCCTTCACAGTATCACATTTGAAACTTTGTGCTGGAATCATGATAACTG
  CATCTCACAATCCAAAGCAGGATAATGGTTATAAGGTCTATTGGGATAATGGAGCTCAGATCATTTCTCC
  TCACGATAAAGGGATTTCTCAAGCTATTGAAGAAAATCTAGAACCGTGGCCTCAAGCTTGGGACGATTCT
  TTAATTGATAGCAGTCCACTTCTCCACAATCCGAGTGCTTCCATCAATAATGACTACTTTGAAGACCTTA
  AAAAGTACTGTTTCCACAGGAGCGTGAACAGGGAGACAAAGGTGAAGTTTGTGCACACCTCTGTCCATGG
  GGTGGGTCATAGCTTTGTGCAGTCAGCTTTCAAGGCTTTTGACCTTGTTCCTCCTGAGGCTGTTCCTGAA
  CAGAAAGATCCGGATCCTGAGTTTCCAACAGTGAAATACCCGAATCCCGAAGAGGGGAAAGGTGTCTTGA
  CTTTGTCTTTTGCTTTGGCTGACAAAACCAAGGCCAGAATTGTTTTAGCTAACGACCCGGATGCTGATAG
  ACTTGCTGTGGCAGAAAAGCAAGACAGTGGTGAATGGAGGGTGTTTTCAGGCAATGAGTTGGGGGCCCTC
  CTGGGCTGGTGGCTTTTTACATCTTGGAAAGAGAAGAACCAGGATCGCAGTGCTCTCAAAGACACGTACA
  TGTTGTCCAGCACCGTCTCCTCCAAAATCTTGCGGGCCATTGCCTTAAAGGAAGGTTTTCATTTTGAGGA
  AACATTAACTGGCTTTAAGTGGATGGGAAACAGAGCCAAACAGCTAATAGACCAGGGGAAAACTGTTTTA
  TTTGCATTTGAAGAAGCTATTGGATACATGTGCTGCCCTTTTGTTCTGGACAAAGATGGAGTCAGTGCCG
  CTGTCATAAGTGCAGAGTTGGCTAGCTTCCTAGCAACCAAGAATTTGTCTTTGTCTCAGCAACTAAAGGC
  CATTTATGTGGAGTATGGCTACCATATTACTAAAGCTTCCTATTTTATCTGCCATGATCAAGAAACCATT
  AAGAAATTATTTGAAAACCTCAGAAACTACGATGGAAAAAATAATTATCCAAAAGCTTGTGGCAAATTTG
  AAATTTCTGCCATTAGGGACCTTACAACTGGCTATGATGATAGCCAACCTGATAAAAAAGCTGTTCTTCC
  CACTAGTAAAAGCAGCCAAATGATCACCTTCACCTTTGCTAATGGAGGCGTGGCCACCATGCGCACCAGT
  GGGACAGAGCCCAAAATCAAGTACTATGCAGAGCTGTGTGCCCCACCTGGGAACAGTGATCCTGAGCAGC
  TGAAGAAGGAACTGAATGAACTGGTCAGTGCTATTGAAGAACATTTTTTCCAGCCACAGAAGTACAATCT
  GCAGCCAAAAGCAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203979 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPEGSGLDEDARLDQETAQWLRWDKNSLTLEAVKRLIAEGNKEELRKCFGARMEFGTAGLRAAMGPGI
  SRMNDLTIIQTTQGFCRYLEKQFSDLKQKGIVISFDARAHPSSGGSSRRFARLAATTFISQGIPVYLFSD
  ITPTPFVPFTVSHLKLCAGIMITASHNPKQDNGYKVYWDNGAQIISPHDKGISQAIEENLEPWPQAWDDS
  LIDSSPLLHNPSASINNDYFEDLKKYCFHRSVNRETKVKFVHTSVHGVGHSFVQSAFKAFDLVPPEAVPE
  QKDPDPEFPTVKYPNPEEGKGVLTLSFALADKTKARIVLANDPDADRLAVAEKQDSGEWRVFSGNELGAL
  LGWWLFTSWKEKNQDRSALKDTYMLSSTVSSKILRAIALKEGFHFEETLTGFKWMGNRAKQLIDQGKTVL
  FAFEEAIGYMCCPFVLDKDGVSAAVISAELASFLATKNLSLSQQLKAIYVEYGYHITKASYFICHDQETI
  KKLFENLRNYDGKNNYPKACGKFEISAIRDLTTGYDDSQPDKKAVLPTSKSSQMITFTFANGGVATMRTS
  GTEPKIKYYAELCAPPGNSDPEQLKKELNELVSAIEEHFFQPQKYNLQPKAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018290
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018290.1, NM_018290.2, NM_018290.3, NP_060760.2
• RefSeq Size: 3238 bp
• RefSeq ORF: 1839 bp
• Locus ID: 55276
• Domains: PGM_PMM_I, PGM_PMM_II
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism
• MW: 68.3 kDa