SH3BP5 (NM_004844) Human Tagged ORF Clone

CAT#: RC203999

SH3BP5 (Myc-DDK-tagged)-Human SH3-domain binding protein 5 (BTK-associated) (SH3BP5), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417710) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203999 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SH3BP5 protein (Cat# TP303999). The protein was produced from HEK293T cells transfected with SH3BP5 cDNA clone (Cat# RC203999) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SH3BP5
• Synonyms: SAB; SH3BP-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203999 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGGCACTGAAGCGGAGCCGCTCGGAGGAGCCAGCCGAAATCCTGCCGCCTGCCCGGGACGAGG
  AGGAGGAGGAGGAAGAGGGGATGGAGCAGGGGCTGGAGGAGGAAGAAGAGGTGGATCCCCGGATCCAGGG
  AGAACTGGAGAAGTTAAATCAGTCCACGGATGATATCAACAGACGGGAGACTGAACTTGAGGATGCTCGT
  CAGAAGTTCCGCTCTGTTCTGGTTGAAGCAACGGTGAAACTGGATGAACTGGTGAAGAAAATTGGCAAAG
  CTGTGGAAGACTCCAAGCCCTACTGGGAGGCACGGAGGGTGGCGAGGCAGGCTCAGCTGGAAGCTCAGAA
  AGCCACGCAGGACTTCCAGAGGGCCACAGAGGTGCTCCGTGCCGCCAAGGAGACCATCTCCCTGGCCGAG
  CAGCGGCTGCTGGAGGATGACAAGCGGCAGTTCGACTCCGCCTGGCAGGAGATGCTGAATCACGCCACTC
  AGAGGGTCATGGAGGCGGAGCAGACCAAGACCAGGAGCGAGCTGGTGCATAAGGAGACGGCAGCCAGGTA
  CAATGCCGCCATGGGCCGCATGCGACAGCTGGAGAAGAAACTCAAGAGAGCCATCAACAAGTCCAAGCCT
  TATTTTGAACTCAAGGCAAAGTACTATGTGCAGCTCGAGCAACTGAAAAAGACTGTGGATGACCTGCAGG
  CCAAACTGACCCTGGCAAAAGGCGAGTACAAGATGGCCCTGAAGAACCTGGAGATGATCTCAGATGAGAT
  CCACGAGCGGCGGCGCTCCAGTGCCATGGGGCCTCGGGGATGCGGTGTTGGTGCTGAGGGCAGCAGCACA
  TCTGTGGAGGATCTGCCAGGGAGCAAACCTGAGCCTGATGCCATTTCTGTGGCCTCGGAGGCCTTTGAAG
  ATGACAGCTGTAGCAACTTTGTGTCTGAAGATGACTCGGAAACCCAGTCCGTGTCCAGCTTTAGTTCAGG
  ACCAACAAGCCCGTCTGAGATGCCTGACCAGTTCCCTGCGGTTGTGAGGCCTGGCAGCCTGGATCTGCCC
  AGCCCTGTGTCCCTGTCAGAGTTTGGGATGATGTTCCCAGTGTTGGGCCCTCGAAGTGAATGCAGCGGGG
  CCTCCTCCCCTGAATGTGAAGTAGAACGAGGAGACAGGGCAGAAGGGGCAGAGAATAAAACAAGTGACAA
  AGCCAACAACAACCGGGGCCTCAGCAGTAGCAGTGGCAGTGGTGGCAGCAGTAAGAGCCAAAGCAGCACC
  TCCCCTGAGGGCCAGGCCTTGGAGAACCGGATGAAGCAGCTCTCCCTACAGTGCTCAAAGGGAAGAGATG
  GAATTATTGCTGACATAAAAATGGTGCAGATTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203999 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDAALKRSRSEEPAEILPPARDEEEEEEEGMEQGLEEEEEVDPRIQGELEKLNQSTDDINRRETELEDAR
  QKFRSVLVEATVKLDELVKKIGKAVEDSKPYWEARRVARQAQLEAQKATQDFQRATEVLRAAKETISLAE
  QRLLEDDKRQFDSAWQEMLNHATQRVMEAEQTKTRSELVHKETAARYNAAMGRMRQLEKKLKRAINKSKP
  YFELKAKYYVQLEQLKKTVDDLQAKLTLAKGEYKMALKNLEMISDEIHERRRSSAMGPRGCGVGAEGSST
  SVEDLPGSKPEPDAISVASEAFEDDSCSNFVSEDDSETQSVSSFSSGPTSPSEMPDQFPAVVRPGSLDLP
  SPVSLSEFGMMFPVLGPRSECSGASSPECEVERGDRAEGAENKTSDKANNNRGLSSSSGSGGSSKSQSST
  SPEGQALENRMKQLSLQCSKGRDGIIADIKMVQIG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004844
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004844.3, NM_004844.4, NP_004835.2
• RefSeq Size: 3322 bp
• RefSeq ORF: 1368 bp
• Locus ID: 9467
• MW: 50.4 kDa