ERp72 (PDIA4) (NM_004911) Human Tagged ORF Clone

CAT#: RC204041

PDIA4 (Myc-DDK-tagged)-Human protein disulfide isomerase family A, member 4 (PDIA4)

Product Images

Western blot validation of overexpression lysate (Cat# LY401531) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204041 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PDIA4 protein (Cat# TP304041). The protein was produced from HEK293T cells transfected with PDIA4 cDNA clone (Cat# RC204041) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDIA4
• Synonyms: ERp-72; ERP70; ERP72
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204041 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCCCCGGAAAGCCTTCCTGCTCCTGCTGCTCTTGGGGCTGGTGCAGCTGCTGGCCGTGGCGGGTG
  CCGAGGGCCCGGACGAGGATTCTTCTAACAGAGAAAATGCCATTGAGGATGAAGAGGAGGAGGAGGAGGA
  AGATGATGATGAGGAAGAAGACGACTTGGAAGTTAAGGAAGAAAATGGAGTCTTGGTCCTAAATGATGCA
  AACTTTGATAATTTTGTGGCTGACAAAGACACAGTGCTGCTGGAGTTTTATGCTCCATGGTGTGGACATT
  GCAAGCAGTTTGCTCCGGAATATGAAAAAATTGCCAACATATTAAAGGATAAAGATCCTCCCATTCCTGT
  TGCCAAGATCGATGCAACCTCAGCGTCTGTGCTGGCCAGCAGGTTTGATGTGAGTGGCTACCCCACCATC
  AAGATCCTTAAGAAGGGGCAGGCTGTAGACTACGAGGGCTCCAGAACCCAGGAAGAAATTGTTGCCAAGG
  TCAGAGAAGTCTCCCAGCCCGACTGGACGCCTCCACCAGAAGTCACGCTTGTGTTGACCAAAGAGAACTT
  TGATGAAGTTGTGAATGATGCAGATATCATTCTGGTGGAGTTTTATGCCCCATGGTGTGGACACTGCAAG
  AAACTTGCCCCCGAGTATGAGAAGGCCGCCAAGGAGCTCAGCAAGCGTTCTCCTCCAATTCCCCTGGCAA
  AGGTCGACGCCACCGCAGAAACAGACCTGGCCAAGAGGTTTGATGTCTCTGGCTATCCCACCCTGAAAAT
  TTTCCGCAAAGGAAGGCCTTATGACTACAACGGCCCACGAGAAAAATATGGAATCGTTGATTACATGATC
  GAGCAGTCCGGGCCTCCCTCCAAGGAGATTCTGACCCTGAAGCAGGTCCAGGAGTTCCTGAAGGATGGAG
  ACGATGTCATCATCATCGGGGTCTTTAAGGGGGAGAGTGACCCAGCCTACCAGCAATACCAGGATGCCGC
  TAACAACCTGAGAGAAGATTACAAATTTCACCACACTTTCAGCACAGAAATAGCAAAGTTCTTGAAAGTC
  TCCCAGGGGCAGTTGGTTGTAATGCAGCCTGAGAAATTCCAGTCCAAGTATGAGCCCCGGAGCCACATGA
  TGGACGTCCAGGGCTCCACCCAGGACTCGGCCATCAAGGACTTCGTGCTGAAGTACGCCCTGCCCCTGGT
  TGGCCACCGCAAGGTGTCAAACGATGCTAAGCGCTACACCAGGCGCCCCCTGGTGGTCGTCTACTACAGT
  GTGGACTTCAGCTTTGATTACAGAGCTGCAACTCAGTTTTGGCGGAGCAAAGTCCTAGAGGTGGCCAAGG
  ACTTCCCTGAGTACACCTTTGCCATTGCGGACGAAGAGGACTATGCTGGGGAGGTGAAGGACCTGGGGCT
  CAGCGAGAGTGGGGAGGATGTCAATGCCGCCATCCTGGACGAGAGTGGGAAGAAGTTCGCCATGGAGCCA
  GAGGAGTTTGACTCTGACACCCTCCGCGAGTTTGTCACTGCTTTCAAAAAAGGAAAACTGAAGCCAGTCA
  TCAAATCCCAGCCAGTGCCCAAGAACAACAAGGGACCCGTCAAGGTCGTGGTGGGAAAGACCTTTGACTC
  CATTGTGATGGACCCCAAGAAGGACGTCCTCATCGAGTTCTACGCACCATGGTGCGGGCACTGCAAGCAG
  CTAGAGCCCGTGTACAACAGCCTGGCCAAGAAGTACAAGGGCCAAAAGGGCCTGGTCATCGCCAAGATGG
  ACGCCACTGCCAACGACGTCCCCAGCGACCGCTATAAGGTGGAGGGCTTCCCCACCATCTACTTCGCCCC
  CAGTGGGGACAAAAAGAACCCAGTTAAATTTGAGGGTGGAGACAGAGATCTGGAGCATTTGAGCAAGTTT
  ATAGAAGAACATGCCACAAAACTGAGCAGGACCAAGGAAGAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204041 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKEENGVLVLNDA
  NFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTI
  KILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCK
  KLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMI
  EQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKV
  SQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYS
  VDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEP
  EEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQ
  LEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKF
  IEEHATKLSRTKEEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004911
• ORF Size: 1935 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004911.1, NM_004911.2, NM_004911.3, NM_004911.4, NP_004902.1
• RefSeq Size: 2970 bp
• RefSeq ORF: 1938 bp
• Locus ID: 9601
• Domains: thiored
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vibrio cholerae infection
• MW: 72.9 kDa
• Gene Summary: This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. [provided by RefSeq, Dec 2016]