BUD23 (NM_017528) Human Tagged ORF Clone

CAT#: RC204043

WBSCR22 (Myc-DDK-tagged)-Human Williams Beuren syndrome chromosome region 22 (WBSCR22), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY413723) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204043 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BUD23
• Synonyms: HASJ4442; HUSSY-3; MERM1; PP3381; WBMT; WBSCR22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204043 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCCGCGGCCGGCGTCCGGAGCATGGCGGACCCCCAGAGCTGTTTTATGACGAGACAGAAGCCC
  GGAAATACGTTCGCAACTCACGGATGATTGATATCCAGACCAGGATGGCTGGGCGAGCATTGGAGCTTCT
  TTATCTGCCAGAGAATAAGCCCTGTTACCTGCTGGATATTGGCTGTGGCACTGGGCTGAGTGGAAGTTAT
  CTGTCAGATGAAGGGCACTATTGGGTGGGCCTGGATATCAGCCCTGCCATGCTGGATGAGGCTGTGGACC
  GAGAGATAGAGGGAGACCTGCTGCTGGGGGATATGGGCCAGGGCATCCCATTCAAGCCAGGCACATTTGA
  TGGTTGCATCAGCATTTCTGCTGTGCAGTGGCTCTGTAATGCTAACAAGAAGTCTGAAAACCCTGCCAAG
  CGCCTGTACTGCTTTTTTGCTTCTCTTTTTTCTGTTCTCGTCCGGGGATCCCGAGCTGTCCTGCAGCTGT
  ACCCTGAGAACTCAGAGCAGTTGGAGCTGATCACAACCCAGGCCACAAAGGCAGGCTTCTCCGGTGGCAT
  GGTGGTAGACTACCCTAACAGTGCCAAAGCAAAGAAATTCTACCTCTGCTTGTTTTCTGGGCCTTCGACC
  TTTATACCAGAGGGGCTGAGTGAAAATCAGGATGAAGTTGAACCCAGGGAGTCTGTGTTCACCAATGAGA
  GGTTCCCATTAAGGATGTCGAGGCGGGGAATGGTGAGGAAGAGTCGGGCATGGGTGCTGGAGAAGAAGGA
  GCGGCACAGGCGCCAGGGCAGGGAAGTCAGACCTGACACCCAGTACACCGGCCGCAAGCGCAAGCCCCGC
  TTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204043 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASRGRRPEHGGPPELFYDETEARKYVRNSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGLSGSY
  LSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQWLCNANKKSENPAK
  RLYCFFASLFSVLVRGSRAVLQLYPENSEQLELITTQATKAGFSGGMVVDYPNSAKAKKFYLCLFSGPST
  FIPEGLSENQDEVEPRESVFTNERFPLRMSRRGMVRKSRAWVLEKKERHRRQGREVRPDTQYTGRKRKPR
  F
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017528
• ORF Size: 843 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017528.2, NM_017528.3, NM_017528.4, NP_059998.2
• RefSeq Size: 1256 bp
• RefSeq ORF: 846 bp
• Locus ID: 114049
• Protein Families: Druggable Genome
• Protein Pathways: Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism
• MW: 31.9 kDa
• Gene Summary: This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternatively spliced transcript variants have been found. [provided by RefSeq, Feb 2011]