NLE1 (NM_018096) Human Tagged ORF Clone

CAT#: RC204114

NLE1 (Myc-DDK-tagged)-Human notchless homolog 1 (Drosophila) (NLE1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413312) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204114 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NLE1 protein (Cat# TP304114). The protein was produced from HEK293T cells transfected with NLE1 cDNA clone (Cat# RC204114) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NLE1
• Synonyms: NLE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204114 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGCAGTGGCGGACGAGGCGGTGGCGCGCGATGTGCAGCGGTTGCTAGTGCAGTTCCAGGATG
  AGGGCGGGCAGCTGCTGGGTTCCCCGTTCGACGTGCCCGTGGACATCACCCCGGACAGGCTGCAGCTCGT
  GTGCAACGCGCTACTGGCCCAGGAGGATCCCCTGCCACTGGCTTTCTTTGTCCACGATGCTGAGATCGTC
  TCCTCACTGGGGAAGACGTTGGAGTCCCAGGCAGTGGAGACAGAGAAGGTCCTAGACATCATCTACCAGC
  CACAGGCTATCTTCAGAGTCCGGGCTGTGACTCGCTGCACCAGCTCCTTGGAGGGTCACAGTGAGGCAGT
  CATTTCTGTGGCCTTCAGCCCTACGGGAAAGTACCTGGCCAGTGGCTCTGGAGACACCACCGTGCGCTTC
  TGGGATCTCAGCACAGAGACACCACATTTCACATGCAAGGGACACAGACACTGGGTCCTTAGTATATCCT
  GGTCTCCAGATGGCAAGAAGCTGGCCTCAGGCTGCAAGAATGGCCAGATTCTCCTCTGGGACCCAAGCAC
  AGGGAAGCAGGTGGGCAGGACCCTCGCTGGCCACAGCAAGTGGATCACAGGCCTGAGCTGGGAGCCCCTC
  CATGCGAACCCTGAGTGCCGCTATGTGGCCAGCAGCTCCAAGGATGGCAGTGTGCGGATCTGGGACACAA
  CTGCAGGCCGCTGTGAGCGCATCCTCACCGGGCACACCCAGTCGGTCACCTGTCTCCGGTGGGGAGGGGA
  CGGGCTTCTCTACTCTGCCTCCCAGGACCGCACCATCAAAGTCTGGAGAGCTCATGACGGTGTGCTGTGC
  CGGACTCTGCAAGGCCACGGCCACTGGGTGAACACCATGGCCCTCAGCACTGACTATGCCCTGCGCACTG
  GGGCCTTTGAACCTGCTGAGGCCTCAGTTAATCCCCAAGACCTCCAAGGATCCTTGCAGGAGTTGAAGGA
  GAGGGCTCTGAGCCGATACAACCTCGTGCGGGGCCAGGGTCCAGAGAGGCTGGTGTCTGGCTCCGACGAC
  TTCACCTTATTCCTGTGGTCCCCAGCAGAGGACAAAAAGCCTCTCACTCGGATGACAGGACACCAAGCTC
  TCATCAACCAGGTGCTCTTCTCTCCTGACTCCCGCATCGTGGCTAGTGCCTCCTTTGACAAGTCCATCAA
  GCTGTGGGATGGCAGGACGGGCAAGTACCTGGCTTCCCTACGCGGCCACGTGGCTGCCGTGTACCAGATT
  GCGTGGTCAGCTGACAGTCGGCTCCTGGTCAGCGGCAGCAGTGACAGCACACTGAAGGTGTGGGATGTGA
  AGGCCCAGAAGCTGGCCATGGACCTGCCCGGCCACGCGGATGAGGTATATGCTGTTGACTGGAGTCCAGA
  TGGCCAGAGAGTGGCAAGTGGTGGGAAGGACAAATGCCTCCGGATATGGAGGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204114 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAVADEAVARDVQRLLVQFQDEGGQLLGSPFDVPVDITPDRLQLVCNALLAQEDPLPLAFFVHDAEIV
  SSLGKTLESQAVETEKVLDIIYQPQAIFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRF
  WDLSTETPHFTCKGHRHWVLSISWSPDGKKLASGCKNGQILLWDPSTGKQVGRTLAGHSKWITGLSWEPL
  HANPECRYVASSSKDGSVRIWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLC
  RTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDD
  FTLFLWSPAEDKKPLTRMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQI
  AWSADSRLLVSGSSDSTLKVWDVKAQKLAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018096
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018096.1, NM_018096.2, NM_018096.3, NM_018096.4, NP_060566.2
• RefSeq Size: 2627 bp
• RefSeq ORF: 1458 bp
• Locus ID: 54475
• Domains: WD40
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 53.3 kDa