RNF31 (NM_017999) Human Tagged ORF Clone

CAT#: RC204117

RNF31 (Myc-DDK-tagged)-Human ring finger protein 31 (RNF31)

Product Images

Western blot validation of overexpression lysate (Cat# LY413390) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204117 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF31
• Synonyms: HOIP; Paul; ZIBRA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204117 representing NM_017999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGGGAGGAAGAGGAGCGGGCCTTCCTGGTGGCCCGCGAGGAGCTGGCGAGCGCCCTGAGGAGGG
  ATTCCGGGCAGGCGTTTTCCCTGGAGCAGCTCCGGCCGCTACTAGCCAGCTCTCTGCCGCTAGCCGCCCG
  CTACCTGCAGCTGGACGCCGCACGCCTTGTCCGCTGCAACGCTCATGGGGAGCCCCGAAACTACCTCAAC
  ACCCTGTCCACGGCTCTGAACATCCTGGAGAAATACGGCCGCAACCTTCTCAGCCCTCAGCGGCCTCGGT
  ACTGGCGTGGTGTCAAGTTTAATAACCCTGTCTTTCGCAGCACGGTGGATGCTGTGCAGGGGGGCCGAGA
  TGTGCTGCGATTATATGGCTACACAGAGGAGCAACCAGATGGGTTGAGCTTCCCCGAAGGGCAGGAGGAG
  CCAGATGAGCACCAGGTTGCTACAGTCACACTGGAAGTACTGCTGCTTCGGACAGAGCTCAGCCTGCTAT
  TGCAGAATACTCATCCAAGACAGCAGGCACTGGAGCAGCTGTTGGAAGACAAGGTTGAAGATGATATGCT
  GCAGCTTTCAGAATTTGACCCCCTATTGAGAGAGATTGCTCCTGGCCCCCTCACCACACCCTCTGTCCCA
  GGCTCCACTCCTGGTCCCTGCTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACTGCCCATCCTGTAAAC
  AGGCCCTGTGTCCAGCCTGTGACCACCTGTTCCATGGACACCCATCCCGTGCTCATCACCTCCGCCAGAC
  CCTGCCTGGGGTCCTGCAGGGTACCCACCTGAGCCCCAGTTTACCTGCCTCAGCCCAACCACGGCCCCAG
  TCGACCTCCCTGCTGGCCCTGGGAGACAGCTCTCTTTCTTCCCCTAATCCTGCAAGTGCTCATTTGCCCT
  GGCACTGTGCTGCCTGTGCCATGCTAAATGAGCCTTGGGCAGTGCTCTGTGTGGCCTGTGATCGGCCCCG
  AGGCTGTAAGGGGTTGGGGTTGGGAACTGAGGGTCCCCAAGGAACTGGAGGCCTAGAACCTGATCTTGCA
  CGGGGTCGGTGGGCCTGCCAGAGCTGTACCTTTGAGAATGAGGCAGCTGCTGTGCTATGTTCCATATGTG
  AGCGACCTCGGCTGGCCCAGCCTCCCAGCTTGGTGGTGGATTCCCGAGATGCTGGCATTTGCCTGCAACC
  CCTTCAGCAGGGGGATGCTTTGCTGGCCTCTGCCCAGAGTCAAGTCTGGTACTGTATTCACTGTACCTTC
  TGCAACTCGAGCCCTGGCTGGGTGTGTGTTATGTGCAACCGGACTAGTAGCCCCATTCCAGCACAACATG
  CCCCCCGGCCCTATGCCAGCTCTTTGGAAAAGGGACCCCCCAAGCCTGGGCCCCCACGACGCCTTAGTGC
  CCCCCTGCCCAGTTCCTGTGGAGATCCTGAGAAGCAGCGCCAAGACAAGATGCGGGAAGAAGGCCTCCAG
  CTAGTGAGCATGATCCGGGAAGGGGAAGCCGCAGGTGCCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGT
  ACTCGGGCACTGAGGTGCCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACGTCCTGGAGATGGTGGCTGA
  GCTGGCTGGACAGCAGGACCCTGGGCTGGGTGCCTTTTCCTGTCAGGAGGCCCGGAGAGCCTGGCTGGAT
  CGTCATGGCAACCTTGATGAAGCTGTGGAGGAGTGTGTGAGGACCAGGCGAAGGAAGGTGCAGGAGCTCC
  AGTCTCTAGGCTTTGGGCCTGAGGAGGGGTCTCTCCAGGCATTGTTCCAGCACGGAGGTGATGTGTCACG
  GGCCCTGACTGAGCTACAGCGCCAACGCCTAGAGCCCTTCCGCCAGCGCCTCTGGGACAGTGGCCCTGAG
  CCCACCCCTTCCTGGGATGGGCCAGACAAGCAGAGCCTGGTCAGGCGGCTTTTGGCAGTCTACGCACTCC
  CCAGCTGGGGCCGGGCAGAGCTGGCACTGTCACTGCTGCAGGAGACACCCAGGAACTATGAGTTGGGGGA
  TGTGGTAGAAGCTGTGAGGCACAGCCAGGACCGGGCCTTCCTGCGCCGCTTGCTTGCCCAGGAGTGTGCC
  GTGTGTGGCTGGGCCCTGCCCCACAACCGGATGCAGGCCCTGACTTCCTGTGAGTGCACCATCTGTCCTG
  ACTGCTTCCGCCAGCACTTCACCATCGCCTTGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTG
  TGGCCGCCCCGACCTCACCGATGACACACAGTTGCTCAGCTACTTCTCTACCCTTGACATCCAGCTTCGC
  GAGAGCCTAGAGCCAGATGCCTATGCGTTGTTCCATAAGAAGCTGACCGAGGGTGTGCTGATGCGGGACC
  CCAAGTTCTTGTGGTGTGCCCAGTGCTCCTTTGGCTTCATATATGAGCGTGAGCAGCTGGAGGCAACTTG
  TCCCCAGTGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCCAGTGGGAGGAGCAGCACCGAGGTCGGAGC
  TGTGAGGACTTCCAGAACTGGAAACGCATGAACGACCCAGAATACCAGGCCCAGGGCCTAGCAATGTATC
  TTCAGGAAAACGGCATTGACTGCCCCAAATGCAAGTTCTCGTACGCCCTGGCCCGAGGAGGCTGCATGCA
  CTTTCACTGTACCCAGTGCCGCCACCAGTTCTGCAGCGGCTGCTACAATGCCTTTTACGCCAAGAATAAA
  TGTCCAGAGCCTAACTGCAGGGTGAAAAAGTCCCTGCACGGCCACCACCCTCGAGACTGCCTCTTCTACC
  TGCGGGACTGGACTGCTCTCCGGCTTCAGAAGCTGCTACAGGACAATAACGTCATGTTTAATACAGAGCC
  TCCAGCTGGGGCCCGGGCAGTCCCTGGAGGCGGCTGCCGAGTGATAGAGCAGAAGGAGGTTCCCAATGGG
  CTCAGGGACGAAGCTTGTGGCAAGGAAACTCCAGCTGGCTATGCCGGCCTGTGCCAGGCACACTACAAAG
  AGTATCTTGTGAGCCTCATCAATGCCCACTCGCTGGACCCAGCCACCTTGTATGAGGTGGAAGAGCTGGA
  GACGGCCACTGAGCGCTACCTGCACGTACGCCCCCAGCCTTTGGCTGGAGAGGATCCCCCTGCTTACCAG
  GCCCGCTTGTTACAGAAGCTGACAGAAGAGGTACCCTTGGGACAGAGTATCCCCCGCAGGCGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204117 representing NM_017999
  Red=Cloning site Green=Tags(s)
  
  MPGEEEERAFLVAREELASALRRDSGQAFSLEQLRPLLASSLPLAARYLQLDAARLVRCNAHGEPRNYLN
  TLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEE
  PDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLLREIAPGPLTTPSVP
  GSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHGHPSRAHHLRQTLPGVLQGTHLSPSLPASAQPRPQ
  STSLLALGDSSLSSPNPASAHLPWHCAACAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLEPDLA
  RGRWACQSCTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQPLQQGDALLASAQSQVWYCIHCTF
  CNSSPGWVCVMCNRTSSPIPAQHAPRPYASSLEKGPPKPGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQ
  LVSMIREGEAAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAWLD
  RHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQALFQHGGDVSRALTELQRQRLEPFRQRLWDSGPE
  PTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECA
  VCGWALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLR
  ESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRS
  CEDFQNWKRMNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNK
  CPEPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNG
  LRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQ
  ARLLQKLTEEVPLGQSIPRRRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017999
• ORF Size: 3216 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017999.2, NM_017999.4, NP_060469.4
• RefSeq Size: 3571 bp
• RefSeq ORF: 3219 bp
• Locus ID: 55072
• Domains: IBR
• Protein Families: Druggable Genome
• MW: 119.5 kDa
• Gene Summary: The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]