ABP1 (AOC1) (NM_001091) Human Tagged ORF Clone

CAT#: RC204163

ABP1 (Myc-DDK-tagged)-Human amiloride binding protein 1 (amine oxidase (copper-containing)) (ABP1)

Product Images

Western blot validation of overexpression lysate (Cat# LY400441) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204163 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AOC1 protein (Cat# TP304163). The protein was produced from HEK293T cells transfected with AOC1 cDNA clone (Cat# RC204163) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AOC1
• Synonyms: ABP; ABP1; DAO; DAO1; KAO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204163 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCCTGGGCTGGGCCGTGGCTGCCATCCTGATGCTGCAGACGGCCATGGCGGAGCCCTCCCCGG
  GGACTCTGCCCAGGAAGGCAGGGGTGTTTTCAGACCTAAGCAACCAAGAGCTGAAGGCAGTGCACAGCTT
  CCTCTGGTCCAAGAAGGAGCTGAGGCTGCAGCCCTCCAGTACCACCACCATGGCCAAGAACACCGTGTTT
  CTCATCGAGATGCTGCTGCCCAAGAAGTACCATGTGCTGAGGTTTCTGGATAAAGGTGAAAGGCATCCTG
  TGCGGGAAGCCCGTGCCGTCATCTTCTTTGGTGACCAGGAGCATCCCAATGTCACCGAGTTTGCTGTGGG
  GCCCCTGCCAGGGCCCTGCTACATGCGAGCACTGTCCCCCAGGCCTGGGTACCAGTCCTCCTGGGCATCG
  AGGCCCATCTCCACAGCAGAGTATGCCCTCCTCTACCACACCCTGCAGGAAGCCACCAAGCCCCTGCATC
  AGTTCTTCCTCAATACCACAGGCTTCTCATTCCAAGACTGCCATGACAGATGCCTGGCCTTCACCGATGT
  GGCCCCCCGGGGTGTGGCTTCTGGCCAGCGCCGCAGTTGGCTTATCATACAGCGCTATGTAGAAGGCTAC
  TTTCTGCACCCCACTGGGCTGGAGCTCCTCGTGGATCATGGGAGCACAGATGCTGGGCACTGGGCCGTGG
  AGCAGGTGTGGTACAACGGGAAGTTCTATGGGAGCCCAGAGGAACTGGCTCGGAAGTATGCAGATGGAGA
  GGTGGACGTGGTGGTCCTGGAGGACCCGCTGCCTGGGGGCAAGGGGCATGACAGCACAGAGGAGCCGCCC
  CTCTTCTCCTCCCACAAGCCCCGCGGGGACTTCCCCAGCCCCATCCATGTGAGCGGCCCCCGCTTGGTCC
  AGCCCCACGGCCCTCGCTTCAGGCTGGAGGGCAACGCTGTGCTCTACGGCGGCTGGAGCTTTGCCTTCCG
  GCTGCGCTCCTCCTCCGGGCTGCAGGTCCTGAACGTGCACTTCGGCGGAGAGCGCATTGCCTATGAGGTC
  AGCGTGCAAGAGGCAGTGGCGCTGTATGGAGGACACACACCTGCAGGCATGCAGACCAAGTACCTCGATG
  TCGGCTGGGGCCTGGGCAGCGTCACTCATGAGTTAGCCCCCGGCATCGACTGCCCGGAGACCGCCACCTT
  CCTGGACACTTTCCACTACTATGATGCCGATGACCCGGTCCATTATCCCCGAGCCCTCTGCCTCTTTGAA
  ATGCCCACAGGGGTGCCCCTTCGGCGGCACTTTAATTCCAACTTTAAAGGTGGCTTCAACTTCTATGCAG
  GGCTGAAGGGCCAGGTGCTGGTGCTGCGGACAACTTCAACTGTCTACAATTATGATTACATTTGGGACTT
  TATCTTCTACCCCAACGGGGTGATGGAGGCCAAGATGCATGCCACTGGCTACGTCCACGCCACCTTCTAC
  ACCCCCGAGGGGCTGCGCCACGGCACTCGCCTGCACACCCACCTGATTGGCAACATACACACTCACTTGG
  TGCACTACCGCGTAGACCTGGATGTGGCAGGCACCAAGAACAGCTTCCAGACACTGCAGATGAAGCTAGA
  AAACATCACCAACCCCTGGAGCCCGAGACACCGCGTGGTCCAGCCAACTCTGGAGCAGACGCAGTACTCC
  TGGGAGCGCCAGGCGGCCTTCCGCTTCAAAAGGAAGCTGCCCAAGTACCTGCTCTTTACCAGCCCCCAGG
  AGAACCCCTGGGGCCACAAGCGCAGCTACCGCCTGCAGATCCACTCCATGGCCGACCAGGTGCTGCCCCC
  AGGCTGGCAGGAGGAGCAGGCCATCACCTGGGCAAGGTACCCCCTGGCAGTGACCAAGTACCGGGAGTCA
  GAGCTGTGCAGCAGCAGCATCTACCACCAGAACGACCCCTGGGACCCGCCCGTGGTCTTTGAGCAGTTTC
  TTCACAACAACGAGAACATTGAAAATGAGGACCTGGTGGCCTGGGTGACGGTGGGCTTCCTGCACATCCC
  CCACTCAGAGGACATTCCCAACACAGCCACACCTGGGAACTCCGTGGGCTTCCTGCTCCGGCCATTCAAC
  TTCTTCCCAGAGGACCCCTCCCTGGCATCCAGAGACACTGTGATCGTGTGGCCTCGGGACAACGGCCCCA
  ACTACGTCCAGCGCTGGATCCCTGAGGACAGGGACTGCTCGATGCCTCCCCCTTTTAGCTACAATGGGAC
  CTATAGACCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204163 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPALGWAVAAILMLQTAMAEPSPGTLPRKAGVFSDLSNQELKAVHSFLWSKKELRLQPSSTTTMAKNTVF
  LIEMLLPKKYHVLRFLDKGERHPVREARAVIFFGDQEHPNVTEFAVGPLPGPCYMRALSPRPGYQSSWAS
  RPISTAEYALLYHTLQEATKPLHQFFLNTTGFSFQDCHDRCLAFTDVAPRGVASGQRRSWLIIQRYVEGY
  FLHPTGLELLVDHGSTDAGHWAVEQVWYNGKFYGSPEELARKYADGEVDVVVLEDPLPGGKGHDSTEEPP
  LFSSHKPRGDFPSPIHVSGPRLVQPHGPRFRLEGNAVLYGGWSFAFRLRSSSGLQVLNVHFGGERIAYEV
  SVQEAVALYGGHTPAGMQTKYLDVGWGLGSVTHELAPGIDCPETATFLDTFHYYDADDPVHYPRALCLFE
  MPTGVPLRRHFNSNFKGGFNFYAGLKGQVLVLRTTSTVYNYDYIWDFIFYPNGVMEAKMHATGYVHATFY
  TPEGLRHGTRLHTHLIGNIHTHLVHYRVDLDVAGTKNSFQTLQMKLENITNPWSPRHRVVQPTLEQTQYS
  WERQAAFRFKRKLPKYLLFTSPQENPWGHKRSYRLQIHSMADQVLPPGWQEEQAITWARYPLAVTKYRES
  ELCSSSIYHQNDPWDPPVVFEQFLHNNENIENEDLVAWVTVGFLHIPHSEDIPNTATPGNSVGFLLRPFN
  FFPEDPSLASRDTVIVWPRDNGPNYVQRWIPEDRDCSMPPPFSYNGTYRPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001091
• ORF Size: 2253 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001091.1, NM_001091.2, NM_001091.3, NP_001082.2
• RefSeq Size: 2446 bp
• RefSeq ORF: 2256 bp
• Locus ID: 26
• Domains: Cu_amine_oxid
• Protein Families: Secreted Protein
• Protein Pathways: Arginine and proline metabolism, Histidine metabolism, Tryptophan metabolism
• MW: 85.3 kDa
• Gene Summary: This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]